A5064151398- Radiopharmaceutical Chemistry and Applications
- Medical Imaging Techniques and Applications
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Treatments and Mutations
- Cancer Research and Treatments
- HER2/EGFR in Cancer Research
- Cytomegalovirus and herpesvirus research
- CAR-T cell therapy research
- Herpesvirus Infections and Treatments
- Prostate Cancer Treatment and Research
- DNA and Nucleic Acid Chemistry
- Biochemical and Molecular Research
- Fluorine in Organic Chemistry
- Pancreatic and Hepatic Oncology Research
- Peptidase Inhibition and Analysis
- Glioma Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Cancer therapeutics and mechanisms
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Bioactive Compounds and Antitumor Agents
Shaukat Khanum Memorial Cancer Hospital and Research Center
2024
The University of Texas MD Anderson Cancer Center
2011-2023
University of Houston
2016
Imaging Center
2009-2011
St. Luke's Episcopal Hospital
2010
Baylor College of Medicine
2009
University of Southern California
1994-2007
The University of Texas at Austin
2007
Southern California University for Professional Studies
2002-2004
University of California, Irvine
1997
The management of CNS tumors is limited by the blood-brain barrier (BBB), a vascular interface that restricts passage most molecules from blood into brain. Here we show phage particles targeted with certain ligand motifs selected in vivo combinatorial peptide library can cross BBB under normal and pathological conditions. Specifically, demonstrated clones displaying an iron-mimic were able to target protein complex transferrin receptor (TfR) through non-canonical allosteric binding mechanism...
The importance of the EGF receptor (EGFR) signaling pathway in development and progression nonsmall cell lung carcinomas (NSCLC) is widely recognized. Gene sequencing studies revealed that a majority tumors responding to EGFR kinase inhibitors harbor activating mutations domain. This underscores need for novel biomarkers diagnostic imaging approaches identify patients who may benefit from particular therapeutic agents with improved efficacy safety profiles. To this goal, we developed...
A no-carrier-added synthesis of 9-[(3-[18F]-fluoro-1-hydroxy-2-propoxy)methyl]-guanine ([18F]-FHPG) is reported. The 9-[(1,3-dihydroxy-2-propoxy)methyl)guanine (DHPG) was converted to 9-[N2,O-bis(methoxytrityl)-3-(tosyl)-2-propoxy-methyl]guanine by treatment with methoxytrityl chloride followed tosylation. tosylate reacted [18F]-KF in the presence kryptofix 2.2.2. produce 3-fluoro-N2-O-bis-(methoxytrityl) derivative. Removal protecting groups acid hydrolysis produced [18F]-FHPG. labeled...
A human-derived intrinsically nonimmunogenic reporter gene was tested for PET imaging of different molecular-genetic processes potential clinical use.The human mitochondrial thymidine kinase type 2 (hTK2) truncated at the N terminus (DeltahTK2), alone or fused with green fluorescent protein (GFP), used preclinical evaluation in a mouse model. The levels enzymatic activity DeltahTK2 and GFP proteins were assessed using radiotracer accumulation prodrug activation assays vitro subcutaneous...
Abstract Hypoxia inducible factor-1 (HIF-1) promotes tumor cell adaptation to microenvironmental stress. HIF-1 is up-regulated in irradiated tumors and serves as a promising target for radiosensitization. We initially confirmed that the orally bioavailable inhibitor PX-478 reduces protein levels signaling vitro dose-dependent manner provides direct radiosensitization of hypoxic cancer cells clonogenic survival assays using C6 glioma, HN5 UMSCCa10 squamous cells, Panc-1 pancreatic...
Rationale : Human CD34 + cells have been used in clinical trials for treatment of myocardial infarction (MI). However, it is unknown how long the persist hearts, whether improvement cardiac function sustained, or what are underlying mechanisms. Objective We sought to track fate injected human hearts severe combined immune deficiency (SCID) mice after experimental MI and determine mechanisms action. Methods Results multimodality molecular imaging SCID MI, selective antibody blocking...
Abstract Synthesis of 2′‐deoxy‐2′‐[ 18 F]fluoro‐5‐methyl‐1‐ β ‐D‐arabinofuranosyluracil ([ F]‐FMAU) is reported. 2‐Deoxy‐2‐[ F]fluoro‐1,3,5‐tri‐O‐benzoyl‐ α ‐D‐arabinofuranose 2 was prepared by the reaction respective triflate 1 with tetrabutylammonium[ F]fluoride. The fluorosugar converted to its 1‐bromo‐derivative 3 and coupled protected thymine 4 . crude product mixture ( 5a 5b ) hydrolyzed in base purified HPLC obtain radiolabeled FMAU 6a radiochemical yield 20–30% decay corrected (d.c.)...
Early identification of esophageal cancer patients who are responding or resistant to combined chemoradiotherapy may lead individualized therapeutic approaches and improved clinical outcomes. We assessed the ability 3'-deoxy-3'-(18)F-fluorothymidine positron emission tomography (FLT-PET) detect early changes in tumor proliferation after experimental models carcinoma.The vitro ex vivo uptake [(3)H]FLT SEG-1 human adenocarcinoma cells were studied at various time points docetaxel plus...
Human sarcomas are rare but diverse malignant tumors derived from mesenchymal tissue. Clinical response to therapy is currently determined by the modified World Health Organization (WHO) criteria or Response Evaluation Criteria in Solid Tumors (RECIST), these standards correlate poorly with sarcoma patient outcome. We introduced ligand-directed particles elements of AAV and phage (AAVP) enable integration tumor targeting molecular imaging. report drug-response monitoring prediction a nude...
The long-term fate of stem cells after intramyocardial delivery is unknown. We used noninvasive, repetitive PET/CT imaging with [18F]FEAU to monitor the (up 5 months) spatial-temporal dynamics MSCs retrovirally transduced sr39HSV1-tk gene (sr39HSV1-tk-MSC) and implanted intramyocardially in pigs induced acute myocardial infarction. Repetitive revealed a biphasic pattern sr39HSV1-tk-MSC dynamics; cell proliferation peaked at 33–35 days injection, periinfarct regions major cardiac lymphatic...
Epigenetic modifications mediated by histone deacetylases (HDACs) play important roles in the mechanisms of different neurologic diseases and HDAC inhibitors (HDACIs) have shown promise therapy. However, pharmacodynamic profiles many HDACIs brain remain largely unknown due to lack validated methods for noninvasive imaging expression-activity. In this study, dynamic PET/CT was performed 4 rhesus macaques using [(18)F]FAHA, a novel substrate, [(18)F]fluoroacetate, major radio-metabolite fused...
We performed parallel investigations in cabozantinib-treated patients a phase II trial and simultaneously patient-derived xenograft (PDX) models to better understand the roles of MET VEGFR2 as targets for prostate cancer therapy.In clinical trial, radiographic imaging serum markers were examined, well molecular tumors from bone biopsies. In mice harboring PDX intrafemurally or subcutaneously, cabozantinib effects on tumor growth, MET, which was silenced, VEGFR2, turnover, angiogenesis,...
Aggressive variant prostate cancers (AVPC) are a clinically defined group of tumors heterogeneous morphologies, characterized by poor patient survival and for which limited diagnostic treatment options currently available. We show that the cell surface 78-kDa glucose-regulated protein (GRP78), receptor binds to phage-display-selected ligands, such as SNTRVAP motif, is candidate target in AVPC. report presence accessibility this clinical specimens from index patients. also demonstrate human...