A5036006060- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Olfactory and Sensory Function Studies
- Medical Imaging Techniques and Applications
- Receptor Mechanisms and Signaling
- Sleep and Wakefulness Research
- Visual perception and processing mechanisms
- Memory and Neural Mechanisms
- Neurotransmitter Receptor Influence on Behavior
- Functional Brain Connectivity Studies
- Inflammatory mediators and NSAID effects
- Estrogen and related hormone effects
- Retinal Development and Disorders
- Eicosanoids and Hypertension Pharmacology
- Circadian rhythm and melatonin
- Radiopharmaceutical Chemistry and Applications
- Transcranial Magnetic Stimulation Studies
- Neuroendocrine regulation and behavior
- Neuropeptides and Animal Physiology
- Stress Responses and Cortisol
- Neural and Behavioral Psychology Studies
- Advanced Neuroimaging Techniques and Applications
- Pluripotent Stem Cells Research
- Chemical Synthesis and Analysis
- EEG and Brain-Computer Interfaces
Kobe Gakuin University
2025
Kyoto University
2017-2024
RIKEN Center for Biosystems Dynamics Research
2015-2024
Hiroshima University
2024
Osaka Metropolitan University
2024
National Institute of Advanced Industrial Science and Technology
2024
Fukushima Medical University
2024
Osaka City University
2004-2024
Osaka University
1986-2023
RIKEN Center for Computational Science
2006-2021
Abstract Whole-organ/body three-dimensional (3D) staining and imaging have been enduring challenges in histology. By dissecting the complex physicochemical environment of system, we developed a highly optimized 3D pipeline based on CUBIC. Based our precise characterization biological tissues as an electrolyte gel, experimentally evaluated broad conditions by using artificial tissue-mimicking material. The combination allows bottom-up design superior protocol that can uniformly label whole...
Induced pluripotent stem cells (iPSCs) provide the potential for autologous transplantation using derived from a patient's own cells. However, immunogenicity of iPSCs or their derivatives has been matter controversy, and up to now there no direct comparison allogeneic in brains humans nonhuman primates. Here, primates, we found that iPSC-derived neurons elicited only minimal immune response brain. In contrast, allografts caused an acquired with activation microglia (IBA-1(+)/MHC class II(+))...
The banking of human leukocyte antigen (HLA)-homozygous-induced pluripotent stem cells (iPSCs) is considered a future clinical strategy for HLA-matched cell transplantation to reduce immunological graft rejection. Here we show the efficacy major histocompatibility complex (MHC)-matched allogeneic neural grafting in brain, which less immune-responsive tissue, using iPSCs derived from an MHC homozygous cynomolgus macaque. Positron emission tomography imaging reveals neuroinflammation...
Transection of the direct cortico-motoneuronal pathway at mid-cervical segment spinal cord in macaque monkey results a transient impairment finger movements. Finger dexterity recovers within few months. Combined brain imaging and reversible pharmacological inactivation motor cortical regions suggest that recovery involves bilateral primary cortex during early stage more extensive contralesional premotor late stage. These changes activation pattern frontal motor-related areas represent an...
The sleep-inducing effect of prostaglandin D2 (PGD2) was studied in five conscious male rhesus monkeys (Macaca mulatta) maintained a 12-hr light/dark cycle. PGD2 infused into the lateral or third ventricle cerebrum slowly and continuously for 6 hr light period. Infusion at 15-2250 pmol/min induced natural sleep as identified by electroencephalogram, electromyogram, electrooculogram, body temperature, heart rate, animal behavior. Although sensitivity to slightly different among individual...
For the safe clinical application of embryonic stem cells (ESCs) for neurological diseases, it is critical to evaluate tumorigenicity and function human ESC (hESC)‐derived neural in primates. We have herein, first time, compared growth hESC‐derived with different stages differentiation implanted brains primate models Parkinson's disease. herein show that residual undifferentiated expressing markers present cell preparation can induce tumor formation monkey brain. In contrast, a matured by...
Abstract Metal complex catalysis within biological systems is largely limited to cell and bacterial systems. In this work, a glycoalbumin–Au III was designed developed that enables organ‐specific, localized propargyl ester amidation with nearby proteins live mice. The targeted reactivity can be imaged through the use of Cy7.5‐ TAMRA‐linked based fluorescent probes. This targeting system could enable exploitation other metal strategies for biomedical clinical applications.
Before induced pluripotent stem cells (iPSCs) can be used to treat neurologic diseases, human iPSC-derived neural must analyzed in the primate brain. In fact, although mouse and iPSCs have been generate dopaminergic (DA) neuron
We have previously reported that xeno-transplanted human ESC-derived retinas are able to mature in the immunodeficient retinal degeneration rodent models, similar allo-transplantations using mouse iPSC-derived retina. The photoreceptors latter developed outer segments and formed synapses with host bipolar cells, driving light responses of ganglion cells. In view clinical application, here we further confirmed competency retina (hiPSC-retina) degenerated rat monkey models.Human iPSC-retinas...
In patients with damage to the primary visual cortex (V1), residual vision can guide goal-directed movements targets in blind field without awareness. This phenomenon has been termed blindsight, and its neural mechanisms are controversial. There should be pathways higher cortices bypassing V1, however some literature propose that signal is mediated by superior colliculus (SC) pulvinar, while others claim dorsal lateral geniculate nucleus (dLGN) transmits signal. Here, we directly test role...
Motivation helps reverse neuronal damage plays a fundamental role in mediating recovery after neurological injuries. Sawada et al. evaluated the potential contribution of nucleus accumbens, brain motivation center, to movement control spinal cord injury monkeys. The activity accumbens during early phase was important for motor performance. Science , this issue p. 98
The question of how intensive motor training restores function after brain damage or stroke remains unresolved. Here we show that the ipsilesional ventral premotor cortex (PMv) and perilesional primary (M1) rhesus macaque monkeys are involved in recovery manual dexterity a lesion M1. A focal hand digit area M1 was made by means ibotenic acid injection. This initially caused flaccid paralysis contralateral but followed functional movements, including precision grip, during course daily...
Decision-making is always coupled with some level of risk, more pathological forms risk-taking decisions manifesting as gambling disorders. In macaque monkeys trained in a high risk–high return (HH) versus low risk–low (LL) choice task, we found that the reversible pharmacological inactivation ventral Brodmann area 6 (area 6V) impaired risk dependency decision-making. Selective optogenetic activation mesofrontal pathway from tegmental (VTA) to aspect 6V resulted stronger preference for HH,...
Prostaglandin D 2 (PGD ) is an extensively studied sleep-promoting substance, but the neuroanatomical basis of PGD -induced sleep only partially understood. To determine potential regions involved in this response, we used Fos immunohistochemistry to identify neurons activated by infusion into subarachnoid space below rostral basal forebrain. increased nonrapid eye movement and induced striking expression ventrolateral preoptic area (VLPO), a cluster that may promote inhibiting...
To assess the involvement of serotonin in symptoms chronic fatigue syndrome, we investigated serotonergic neurotransmitter system syndrome patients by positron emission tomography (PET). Here show that density transporters (5-HTTs) brain, as determined using a radiotracer, [C](+)McN5652, was significantly reduced rostral subdivision anterior cingulate compared with normal volunteers. This is different from dorsal which binding potential values individual patient showed weak negative...
Cyclooxygenase (COX)-1 and -2 are prostanoid-synthesizing enzymes that play important roles in the regulation of neuroinflammation development neurodegenerative disorders. However, specific functions these isoforms still unclear. We recently developed <sup>11</sup>C-labeled ketoprofen methyl ester as a PET probe targets COXs for imaging neuroinflammation, though its responsible isoform is yet to be determined. In present study, we performed ex vivo studies with <sup>11</sup>C-ketoprofen...