A5005075596- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- bioluminescence and chemiluminescence research
- Cell Image Analysis Techniques
- Protein Kinase Regulation and GTPase Signaling
- Photosynthetic Processes and Mechanisms
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photochromic and Fluorescence Chemistry
- Cellular transport and secretion
- Optical Coherence Tomography Applications
- 3D Printing in Biomedical Research
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Laser Material Processing Techniques
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Biotin and Related Studies
- Pluripotent Stem Cells Research
- Retinal Development and Disorders
- Advanced Biosensing Techniques and Applications
- Nonlinear Optical Materials Studies
- Microtubule and mitosis dynamics
- Nanofabrication and Lithography Techniques
- Advanced Electron Microscopy Techniques and Applications
- Neural dynamics and brain function
RIKEN Center for Brain Science
2016-2025
RIKEN
2010-2025
RIKEN Center for Advanced Photonics
2016-2025
Kyoto University
1992-2024
RIKEN Center for Biosystems Dynamics Research
2007-2023
The University of Tokyo
1992-2021
Japan Science and Technology Agency
2008-2020
Nitto RIKEN (Japan)
2007-2020
Olympus (Japan)
2018
Advanced Technology Group (Czechia)
2014
Fluorescence resonance energy transfer (FRET) technology has been used to develop genetically encoded fluorescent indicators for various cellular functions. Although most have cyan- and yellow-emitting proteins (CFP YFP) as FRET donor acceptor, their poor dynamic range often prevents detection of subtle but significant signals. Here, we optimized the relative orientation two chromophores in Ca 2+ indicator, yellow cameleon (YC), by fusing YFP at different angles. We generated circularly...
We have cloned a gene encoding fluorescent protein from stony coral, Trachyphyllia geoffroyi , which emits green, yellow, and red light. The protein, named Kaede, includes tripeptide, His-Tyr-Gly, that acts as green chromophore can be converted to red. fluorescence is comparable in intensity the stable under usual aerobic conditions. found green-red conversion highly sensitive irradiation with UV or violet light (350–400 nm), excites protonated form of chromophore. excitation lights used...
To visualize Ca 2+ -dependent protein–protein interactions in living cells by fluorescence readouts, we used a circularly permuted green fluorescent protein (cpGFP), which the amino and carboxyl portions had been interchanged reconnected short spacer between original termini. The cpGFP was fused to calmodulin its target peptide, M13. chimeric protein, have named “pericam,” spectral properties changed reversibly with amount of , probably because interaction M13 leading an alteration...
Cameleons are genetically-encoded fluorescent indicators for Ca 2+ based on green protein variants and calmodulin (CaM). Because cameleons can be targeted genetically imaged by one- or two-photon excitation microscopy, they offer great promise monitoring in whole organisms, tissues, organelles, submicroscopic environments which measurements were previously impossible. However, the original suffered from significant pH interference, their -buffering cross-reactivity with endogenous CaM...
The observation of the regulation fast protein dynamics in a cellular context requires development reliable technologies. Here, signal cascade reliant on stimulus-dependent acceleration bidirectional flow mitogen-activated kinase (extracellular signal-regulated kinase) across nuclear envelope was visualized by reversible highlighting. Light-induced conversion between bright and dark states monomeric fluorescent engineered from novel coral employed. Because its photochromic properties, could...
Mesenchymal stem cells (MSCs) are defined as that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue culture, the equivalent not identified vivo little is known about their physiological roles or even exact location. In this study, we used phenotypic, morphological, functional criteria identify prospectively isolate a subset of (PDGFRα+Sca-1+CD45−TER119−) adult mouse bone marrow. Individual generated colonies...
Autophagy is an essential process for physiological homeostasis, but its role in viral infection only beginning to be elucidated. We show here that the Atg5-Atg12 conjugate, a key regulator of autophagic process, plays important innate antiviral immune responses. Atg5-deficient mouse embryonic fibroblasts (MEFs) were resistant vesicular stomatitis virus replication, which was largely due hyperproduction type I interferons response immunostimulatory RNA (isRNA), such as virus-derived,...
Reversible photoswitching of individual molecules has been demonstrated for a number mutants the green fluorescent protein (GFP). To date, however, limited switching events with slow response to light have achieved at single-molecule level. Here, we report reversible characteristics observed in Dronpa, mutant GFP-like that was cloned from coral Pectiniidae. Ensemble spectroscopy shows intense irradiation 488 nm changes Dronpa dim protonated form, but even weak 405 restores it bright...
Improved spy tactics for single cells Bioluminescence imaging is a tremendous asset to medical research, providing way monitor living noninvasively within their natural environments. Advances in methods allow researchers measure tumor growth, visualize developmental processes, and track cell-cell interactions. Yet technical limitations exist, it difficult image deep tissues or detect low cell numbers vivo. Iwano et al. designed bioluminescence system that produces brighter emission by up...
Kaede is a photoconvertible fluorescence protein that changes from green to red upon exposure violet light. The photoconversion of intracellular has no effect on cellular function. Using transgenic mice expressing the protein, we demonstrated movement cells with photoconverted could be monitored lymphoid organs other tissues as well skin draining lymph node. Analysis kinetics revealed each subset in node, such CD4 + T, CD8 B, and dendritic cells, distinct migration pattern vivo . Thus, mouse...