A5087333755- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- GaN-based semiconductor devices and materials
- Erythrocyte Function and Pathophysiology
- Neurogenesis and neuroplasticity mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Neuroscience and Neural Engineering
- Radio Frequency Integrated Circuit Design
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Cellular transport and secretion
- Crystallization and Solubility Studies
- Dyeing and Modifying Textile Fibers
- Silicon Carbide Semiconductor Technologies
- X-ray Diffraction in Crystallography
- Lipid Membrane Structure and Behavior
- Spine and Intervertebral Disc Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Pancreatic function and diabetes
- Ion Channels and Receptors
- Hair Growth and Disorders
- Advanced Photocatalysis Techniques
- Microbial Metabolic Engineering and Bioproduction
- S100 Proteins and Annexins
Waseda University
2016-2025
Toranomon Hospital
2022
Oita Prefectural Hospital
2021
Takao Hospital
2021
The University of Tokyo
2003-2020
Kumamoto University
2004-2020
Kyushu University
2006-2020
Railway Technical Research Institute
1990-2019
Murata (Japan)
2013-2019
Tsukuba Memorial Hospital
2017-2019
AlGaN-GaN heterojunction field-effect transistors (HFETs) with a field modulating plate (FP) were fabricated on an SiC substrate. The gate-drain breakdown voltage (BV/sub gd/) was significantly improved by employing FP electrode, and the highest BV/sub gd/ of 160 V obtained length (L/sub FP/) 1 μm. maximum drain current achieved 750 mA/mm, together negligibly small collapse. A 1-mm-wide FP-FET FP/=1 μm) biased at 65 demonstrated continuous wave saturated output power 10.3 W linear gain 18.0...
The inositol 1,4,5-trisphosphate receptor (InsP3R) is an intracellular Ca2+ channel that releases from internal stores in response to InsP3. Although InsP3R highly expressed various regions of the mammalian brain, functional role this has not been clarified. We show here cerebellar slices prepared mice with a disrupted type 1 gene, which predominantly Purkinje cells, completely lack long-term depression (LTD), model synaptic plasticity cerebellum. Moreover, specific antibody against InsP3R1,...
Several phospholipase C (PLC) isoforms have been found in male and female mammalian gametes, splicing of PLCdelta4 are predominantly expressed testis. Here we report that mice which the gene had disrupted either produced few small litters or were sterile. In vitro fertilization studies showed insemination with PLCdelta4-/- sperm resulted significantly fewer eggs becoming activated calcium transients associated absent delayed. unable to initiate acrosome reaction, an exocytotic event required...
Various hormonal stimuli and growth factors activate the mammalian canonical transient receptor potential (TRPC) channel through phospholipase C (PLC) activation. However, precise mechanism of regulation TRPC activity remains unknown. Here, we provide first evidence that direct tyrosine phosphorylation by Src family protein-tyrosine kinases (PTKs) is a novel for modulating TRPC6 activity. We found tyrosine-phosphorylated in COS-7 cells when coexpressed with Fyn, member PTKs. also Fyn...
Mitochondrial thermodynamics is the key to understand cellular activities related homeostasis and energy balance.
Cyclic AMP is a ubiquitous second messenger, which mediates many cellular responses mainly initiated by activation of cell surface receptors. Various Förster resonance energy transfer-based ratiometric cAMP indicators have been created for monitoring the spatial and temporal dynamics at single-cell level. However, single fluorescent protein-based poorly developed, with improvement required dynamic range brightness. Based on our previous yellow indicator, Flamindo, we developed an improved...
Abstract The astrocyte, one of the glial cells, plays many functional roles. These include provision nutrients from blood vessels to neurons, supply neurotransmitters and support blood–brain barrier (BBB) integrity. Astrocytes are known integrity BBB through maintenance tight junction between endothelial cells vessels. However, evidence its direct contribution is lacking owing technical limitations. In this study, astrocytic endfeet covering were removed by laser ablation method with two...
Estradiol increases spine density and NMDA-dependent Ca 2+ transients in spines of CA1 pyramidal neurons from hippocampal slices. To investigate the physiological consequences increase induced by estradiol hippocampus, we performed simultaneous whole cell recordings imaging neuron dendrites Four- to eight-days vitro slice cultures were exposed 17β-estradiol (EST) for an additional 4- 8-day period, was assessed confocal microscopy DiI-labeled neurons. Spine doubled both apical basal region...
The inositol 1,4,5-trisphosphate (IP 3 ) receptor R) acts as a Ca 2+ release channel on internal stores. Type 1 IP R R1) is enriched in growth cones of neurons chick dorsal root ganglia. Depletion stores and inhibition signaling with drugs inhibited neurite extension. Microinjection heparin, competitive blocker, induced retraction. Acute localized loss function R1 the cone by chromophore-assisted laser inactivation resulted arrest -induced appears to have crucial role control nerve growth.
Zona pellucida (ZP)–induced acrosome reaction in sperm is a required step for mammalian fertilization. However, the precise mechanism of remains unclear. We previously reported that PLCδ4 involved ZP-induced mouse sperm. Here we have monitored Ca2+ responses single sperm, and report [Ca2+]i increase response to ZP, which essential driving vivo, absent PLCδ4−/− Progesterone, another physiological inducer reaction, failed induce sustained increases consequently was partially inhibited. In...
Various receptors coupled to the heterotrimeric guanine nucleotide-binding protein Gq/11 stimulate formation of inositol-1,4,5-trisphosphate (IP 3 ). Activation these also induces tyrosine phosphorylation. Formation IP in response stimulated that couple was blocked by kinase inhibitors. These inhibitors appeared act before activation Gq/11. Moreover, stimulation induced phosphorylation on a residue (Tyr 356 ) Gα q/11 subunit, and this event essential for activation. Tyrosine changes its...
Undoped β-Ga2O3 single crystals were grown by the floating zone technique under different conditions, and subsequently annealed in O2. Excitation luminescence measurements correlated with optical absorption, Hall- electron-paramagnetic resonance measurements. It is shown, that UV does not have an intrinsic nature, as stated other authors. blue are related to concentration of oxygen vacancies, thus electrical conductivity. They assigned donor-acceptor transitions. On contrary, a green was...
Three subtypes of inositol 1,4,5-trisphosphate receptor (IP3R1, IP3R2, and IP3R3) Ca2+ release channel share basic properties but differ in terms regulation. To what extent they contribute to complex signaling, such as oscillations, remains largely unknown. Here we show that HeLa cells express comparable amounts IP3R1 IP3R3, knockdown by RNA interference each subtype results dramatically distinct signaling patterns. Knockdown significantly decreases total signals terminates oscillations....
Ca(2+) is known to have important roles in sperm chemotaxis, although the relationship between intracellular concentration ([Ca(2+)](i)) and modulation of swimming chemotactic behavior spermatozoa has not been elucidated. Using a high-speed imaging system, we examined [Ca(2+)](i) individual ascidian cells exhibiting responses toward activating attracting factor (SAAF), chemoattractant released by eggs. In this study, found that transient increased flagellum (Ca(2+) bursts) concomitantly with...
Cilia are structurally and functionally diverse organelles, whose malfunction leads to ciliopathies. While recent studies have uncovered common ciliary transport mechanisms, limited information is available on the proteome of cilia, particularly that sensory subtypes, which could provide insight into their functional developmental diversities. In present study, we performed proteomic analysis unique, multiple 9+0 cilia in choroid plexus epithelial cells (CPECs). The juvenile swine CPEC...
Efficiency of synaptic transmission within the neocortex is regulated throughout life by experience and activity. Periods correlated or uncorrelated presynaptic postsynaptic activity lead to enduring changes in efficiency [long-term potentiation (LTP) long-term depression (LTD), respectively]. The initial plasticity triggering event thought be a precipitous rise intracellular calcium, with higher levels inducing LTP more moderate LTD. We used pairing protocol visual cortical brain slices...
The <i>N</i>-methyl-d-aspartate (NMDA) receptor plays important roles in synaptic plasticity and brain development. NMDA subunits have large intracellular domains the COOH-terminal region that may interact with signal-transducing proteins. By using yeast two-hybrid system, we found calmodulin interacts COOH terminus of NR1 subunit inactivates channels a Ca<sup>2+</sup>-dependent manner. Here show protein kinase C (PKC)-mediated phosphorylation on serine residues decreases its affinity for...
We developed genetically encoded fluorescent inositol 1,4,5-trisphosphate (IP3) sensors that do not severely interfere with intracellular Ca2+ dynamics and used them to monitor the spatiotemporal of both cytosolic IP3 in single HeLa cells after stimulation exogenously expressed metabotropic glutamate receptor 5a or endogenous histamine receptors. started increase at a relatively constant rate before pacemaker rise, subsequent abrupt rise was accompanied by any acceleration IP3. Cytosolic...
A recessed-gate AlGaN-GaN field-modulating plate (FP) field-effect transistor (FET) was successfully fabricated on an SiC substrate. By employing a structure FP FET, the transconductance increased from 150 to 270 mS/mm, leading improvement in gain characteristics, and current collapse minimized. At 2 GHz, 48-mm-wide recessed FET exhibited record output power of 230 W (4.8 W/mm) with 67% power-added efficiency 9.5-dB linear drain bias 53 V.
mRNA transport and local translation in the neuronal dendrite is implicated induction of synaptic plasticity. Recently, we cloned an RNA-interacting protein, SYNCRIP (heterogeneous nuclear ribonuclear protein Q1/NSAP1), that suggested to be important for stabilization mRNA. We report here a component granules rat hippocampal neurons. was mainly found at cell bodies, but punctate expression patterns proximal were also seen. Time-lapse analysis living neurons revealed labeled with fluorescent...