A5027414510- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Optical Coherence Tomography Applications
- Optical measurement and interference techniques
- bioluminescence and chemiluminescence research
- Photoacoustic and Ultrasonic Imaging
- Cell Image Analysis Techniques
- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
- Click Chemistry and Applications
- 3D Printing in Biomedical Research
- Photochromic and Fluorescence Chemistry
- Circadian rhythm and melatonin
- Protein Kinase Regulation and GTPase Signaling
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience and Neural Engineering
- Light effects on plants
- Caveolin-1 and cellular processes
- Gene Regulatory Network Analysis
- Advanced MEMS and NEMS Technologies
- Near-Field Optical Microscopy
- Force Microscopy Techniques and Applications
- RNA Research and Splicing
- Marine Ecology and Invasive Species
- Neurobiology and Insect Physiology Research
RIKEN Center for Brain Science
2004-2018
RIKEN
1998-2008
Saitama University
1998-2000
Optica
1998
Tokyo University of Science
1996
Improved spy tactics for single cells Bioluminescence imaging is a tremendous asset to medical research, providing way monitor living noninvasively within their natural environments. Advances in methods allow researchers measure tumor growth, visualize developmental processes, and track cell-cell interactions. Yet technical limitations exist, it difficult image deep tissues or detect low cell numbers vivo. Iwano et al. designed bioluminescence system that produces brighter emission by up...
The structural basis for the photochromism in fluorescent protein Dronpa is poorly understood, because crystal structures of bright state did not provide an answer to mechanism photochromism, and determination dark has been elusive. We performed NMR analyses solution at ambient temperatures find flexibility state. Light-induced changes interactions between chromophore β-barrel are responsible switching two states. In state, apex tethers barrel by a hydrogen bond, imidazole ring protruding...
Protein-protein interactions (PPIs) are essential components of cellular function. Current fluorescence-based technologies to measure PPIs have limited dynamic range and quantitative reproducibility. Here, we describe a genetically-encoded PPI visualization system that harnesses the dynamics condensed liquid-phase transitions analyze protein in living cells. The fluorescent Azami-Green p62-PB1 domain when fused partners triggered rapid concatenation/oligomerization process drove condensation...
We propose a novel technique for simultaneous measurement of layer thicknesses and refractive indices multiple layers. It is based on combination confocal microscope low-coherence interferometry. derived an expression the geometrical thickness index each from both tracing marginal ray accepted by objective optical path matching conditions. Experimental verification this method illustrated several samples that have maximum 13 The thus agreed well with those measured micrometer or cited literature.
The current utility of bioluminescence imaging is constrained by a low photon yield that limits temporal sensitivity. Here, we describe an method uses chemiluminescent/fluorescent protein, ffLuc-cp156, which consists yellow variant Aequorea GFP and firefly luciferase. We report improvement in over three orders magnitude bioluminescent systems. imaged cellular movement at high resolution including neuronal growth cones microglial cell protrusions. Transgenic ffLuc-cp156 mice enabled...
An improved system for the separate measurement of refractive index and geometrical thickness that constitutes a hybrid configuration confocal microscope wavelength-scanning heterodyne interferometer with laser diode is presented. The optical path difference can be measured in less than 1 s, which 10 times quicker low-coherence interferometry previously used, resolution µm fixed reference mirror. Separate glass plates was demonstrated by use arrangement place used previously.
Highly efficient fluorescence resonance energy transfer between cyan(CFP) and yellow fluorescent proteins (YFP), the cyan- yellow-emitting variants of Aequorea green protein, respectively, was achieved by tightly concatenating two proteins. After C-terminus CFP N-terminus YFP were truncated 11 5 amino acids, fused through a leucine-glutamate dipeptide. The resulting chimeric which we called Cy11.5, exhibited simple emission spectrum that peaked at 527 nm when protein excited 436 nm....
We have developed a whole-field fluorescence microscope equipped with Digital Micromirror Device to acquire optically sectioned images by using the fringe-projection technique and phase-shift method. This system allows free control of optical sectioning strength through computer-controlled alteration fringe period projected onto sample. employed this image viable cells expressing fluorescent proteins discussed its biological applications.
We describe the development of first cell-membrane impermeable coelenterazine derivative (CoelPhos). CoelPhos was constructed by alkylation with a linker containing terminal anionic phosphonate moiety. The bioluminescence activity Gaussia luciferase (GLuc) showed significantly higher in comparison Renilla luciferase. In imaging studies living cells, outer membrane bound GLuc clearly imaged CoelPhos. On other hand no signal could be detected intracellularly localized GLuc, demonstrating...
Although the consequences of Ras activation have been studied extensively in context oncogenesis, its regulation physiological modes signal transduction is not well understood. A fluorescent indicator, Raichu-Ras, was fused to C-terminal hypervariable regions H-Ras and K-Ras create indicators for within caveolae/rafts (Raichu-tH) non-raft domains (Raichu-tK) plasma membrane, respectively. Raichu-tH also found abundantly endomembranes. To monitor with high spatial resolution, it imperative...
Dual-excitation ratiometric dyes are excited alternately at two different wavelengths, but the emission is collected a single fixed wavelength. Therefore, pair of intensity measurements must be sequentially. Ratiometric-pericam fluorescent Ca(2+) indicator based on chimeric fusion protein circularly permuted green and calmodulin. Upon binding to calcium, its excitation peak shifts from 415 nm 494 nm. imaging using ratiometric-pericam was thought inadequate follow very fast dynamics or...
Dual-excitation ratiometric dyes are excited alternately at two different wavelengths, but the emission is collected a single fixed wavelength. Therefore, pair of intensity measurements must be sequentially. Ratiometric-pericam fluorescent Ca2+ indicator based on chimeric fusion protein circularly permuted green and calmodulin. Upon binding to calcium, its excitation peak shifts from 415 nm 494 nm. imaging using ratiometric-pericam was thought inadequate follow very fast dynamics or changes...
We developed a heterodyne interference confocal microscope, using wavelength modulation of laser diode to realize quick separate measurement the refractive indices and geometrical thicknesses multiple layers. This microscope requires only single axial movement specimen. can display cross sections interfaces three-layered object.
We previously proposed a system based on combination of confocal microscope and wavelength scanning heterodyne interferometer, for separate measurement refractive index geometrical thickness lens plates. However, we used two optical systems successively although they share the same system. In this paper, propose new scheme which enable us to measure both profile path difference interferometer by single an object. describe herE principle, apparatus, signal processing means, some experimental results.