A5083504396- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Spaceflight effects on biology
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Neuroendocrine regulation and behavior
- Atmospheric chemistry and aerosols
- CRISPR and Genetic Engineering
- Light effects on plants
- Mitochondrial Function and Pathology
- Olfactory and Sensory Function Studies
- Tryptophan and brain disorders
- Alzheimer's disease research and treatments
- Gut microbiota and health
- Neuroscience and Neuropharmacology Research
- Atmospheric aerosols and clouds
- Physiological and biochemical adaptations
- Retinal Development and Disorders
- Chromosomal and Genetic Variations
- Gas Sensing Nanomaterials and Sensors
- Pharmacological Effects of Natural Compounds
- Dietary Effects on Health
- Photosynthetic Processes and Mechanisms
- Evolution and Genetic Dynamics
- Atmospheric Ozone and Climate
Nagoya University
2015-2024
National Institute of Advanced Industrial Science and Technology
2023-2024
Chinese Institute for Brain Research
2024
Neuroscience Institute
2018-2023
Japan Science and Technology Agency
2005-2015
Laboratory of Molecular Genetics
2012
Graduate School USA
2012
Centre de Recherche en Économie et Statistique
2012
Centre for Research in Engineering Surface Technology
2011-2012
Center for Responsible Travel
2012
Temperature is an unavoidable environmental cue that affects the metabolism and behavior of any creature on Earth, yet how animals perceive temperature poorly understood. The nematode Caenorhabditis elegans "memorizes" temperatures, this stored information modifies its subsequent migration along a gradient. We show olfactory neuron designated AWC senses temperature. Calcium imaging revealed responds to changes response thresholds differ depending which animal was previously exposed. In...
Recent developments of imaging techniques have enabled fluorescence microscopy to investigate the localization and dynamics intracellular substances interest even at single-molecule level. However, such sensitive detection is often hampered by autofluorescence arising from endogenous molecules. Those unwanted signals are generally reduced utilizing differences in either wavelength or lifetime; nevertheless, extraction signal insufficient, particularly for vivo imaging. Here, we describe a...
Abstract The nematode Caenorhabditis elegans senses temperature primarily via the AFD thermosensory neurons in head. response to can be observed as a behavior called thermotaxis on thermal gradients. It has been shown that cyclic nucleotide-gated ion channel (CNG channel) plays critical role thermosensation AFD. To further identify mechanisms AFD, we attempted components function upstream of CNG by reverse genetic approach. Genetic and behavioral analyses showed three members subfamily gcy...
Mutations in the unc-51 gene of nematode Caenorhabditis elegans result various abnormalities axonal elongation and structures. We cloned by tagging with transposon Tc1. The wild-type gene, which rescued mutant phenotypes, encodes a novel serine/threonine kinase 856 amino acids. Mutation sites were identified six mutants. A Lys-->Met mutation created vitro domain led to loss rescuing activity was dominant negative, indicating that Unc-51 is essential for function. Expression an unc-51/lacZ...
Animals modify behavioral outputs in response to environmental changes. C. elegans exhibits thermotaxis, where well-fed animals show attraction their cultivation temperature on a thermal gradient without food. We here that feeding-state-dependent modulation of thermotaxis is powerful paradigm for elucidating the mechanism underlying neural plasticity, learning, and memory higher animals. Starved experience alone could induce aversive temperature. Changing both feeding state simultaneously...
Caenorhabditis elegans exhibits a food-associated behavior that is modulated by the past cultivation temperature. Mutations in INS-1, homolog of human insulin, caused defect this integrative behavior. DAF-2/insulin receptor and AGE-1/phosphatidylinositol 3 (PI-3)-kinase partially suppressed ins-1 mutants, mutation DAF-16, forkhead-type transcriptional factor, weak defect. In addition, mutations secretory protein HEN-1 showed synergistic effects with INS-1. Expression AGE-1 any three...
ABSTRACT Restriction endonuclease analysis of mtDNA was used to examine the genetic relatedness several geographically separated isolines Drosophila mercatorum subgroup. In addition, we examined temporal and spatial distribution two restriction site polymorphisms produced by enzymes BstEII BstNI at a single locality—Kamuela, Hawaii. Due small sample sizes some collections undesirable dependance estimation polymorphism frequency on its variance, an arcsin square root transformation data used....
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neurons. We previously showed that expression dynactin 1, an axon protein regulating retrograde transport, markedly reduced in spinal neurons sporadic ALS patients, although mechanisms which decreased 1 levels cause neurodegeneration have yet to be elucidated. The accumulation autophagosomes degenerated another key pathological feature ALS. Since are cargo dynein/dynactin...
Unveiling the neural codes for intricate behaviours is a major challenge in neuroscience. The circuit temperature-seeking behaviour of Caenorhabditis elegans an ideal system to dissect how neurons encode sensory information execution behavioural output. Here we show that temperature-sensing neuron AFD transmits both stimulatory and inhibitory signals single interneuron AIY. In this circuit, calcium concentration threshold acts as switch opposing direct opposite behaviours. Remote control...
Highlights•A single thermosensory neuron is capable of memorizing a temperature•This single-cell memory shows variability between individuals•The CaMKI/IV and Raf pathway modulates in memory•The the underlies premotor activitySummaryUnveiling molecular cellular mechanisms underlying has been challenge for past few decades. Although synaptic plasticity proven to be essential formation, significance "single-cell memory" still remains elusive. Here, we exploited primary culture system analysis...