A5059427033- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neuroscience and Neural Engineering
- Virus-based gene therapy research
- Motor Control and Adaptation
- EEG and Brain-Computer Interfaces
- Photoreceptor and optogenetics research
- Transcranial Magnetic Stimulation Studies
- Neurological disorders and treatments
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Muscle activation and electromyography studies
- Rabies epidemiology and control
- Retinal Development and Disorders
- Peripheral Nerve Disorders
- AI in cancer detection
- Nerve injury and regeneration
- Neurotransmitter Receptor Influence on Behavior
- Digital Radiography and Breast Imaging
- Primate Behavior and Ecology
- Hepatitis C virus research
- Visual perception and processing mechanisms
- Neural and Behavioral Psychology Studies
- Botulinum Toxin and Related Neurological Disorders
Kyoto University
2016-2025
Osaka Hospital
2024
RIKEN Center for Biosystems Dynamics Research
2024
Japan Science and Technology Agency
2010-2023
Inuyama Chuo Hospital
2023
Japan Agency for Medical Research and Development
2022
Institute of Primate Research
2021
Shonan Kamakura General Hospital
2015-2019
Medical & Biological Laboratories (Japan)
2017-2019
National Center of Neurology and Psychiatry
2017
The differentiation of mesenchymal cells into chondrocytes and chondrocyte proliferation maturation are fundamental steps in skeletal development. Runx2 is essential for osteoblast involved maturation. Although delayed Runx2-deficient (Runx2(-/-)) mice, terminal does occur, indicating that additional factors We investigated the involvement Runx3 by generating Runx2-and-Runx3-deficient (Runx2(-/-)3(-/-)) mice. found was inhibited depending on dosages Runx3, Runx2(-/-)3(-/-) mice showed a...
A series of conditional reporter mouse lines were established in which specific organelles labeled with fluorescent proteins. Subcellular localization and intensity 28 fusion-protein constructs surveyed cell lines, 16 then selected to generate lines. The fusion cDNAs inserted into the ROSA26 genomic locus next stop sequences flanked loxP so that proteins expressed under ubiquitous transcriptional machinery when recombined Cre. subcellular product each line examined by ubiquitously expressing...
We found an important piece of evidence for magnetic reconnection inflow in a flare on 1999 March 18. The occurred the northeast limb, displaying nice cusp-shaped soft X-ray loop and plasmoid ejection typical long-duration events. EUV observation same shows us bubble-like void ejection. core this corresponds to plasmoid. Moreover, as is ejected, occurs at disconnecting point. A clear ongoing pattern toward X-point seen. velocity apparent motion about 5 km s-1, which upper limit speed. Based...
Genes involved in the transforming growth factor beta (TGF-beta) signaling pathway are frequently altered several types of cancers, and a gastric tumor suppressor RUNX3 appears to be an integral component this pathway. We reported previously that apoptosis is notably reduced Runx3-/- epithelial cells. In present study, we show proapoptotic gene Bim was transcriptionally activated by cancer cell lines SNU16 SNU719 treated with TGF-beta. The human promoter contains RUNX sites, which required...
Fucci technology makes possible the distinction between live cells in G(1) and S/G(2)/M phases by dual-color imaging. This relies upon ubiquitylation-mediated proteolysis, transgenic mice expressing provide a powerful model system with which to study coordination of cell cycle development. The were initially generated using CAG promoter; lines phase probes that emitted orange (mKO2) green (mAG) fluorescence, respectively, separately constructed. Owing type-biased strength promoter as well...
The lentiviral vector system based on human immunodeficiency virus type 1 (HIV-1) is used extensively in gene therapy trials of neurological and neurodegenerative diseases. Retrograde axonal transport viral vectors offers a great advantage to the delivery genes into neuronal cell bodies that are situated regions distant from injection site. Pseudotyping HIV-1-based with selective variants rabies glycoprotein (RV-G) increases transfer via retrograde central nervous system. Because large-scale...
Abstract The rostromedial caudate (rmCD) of primates is thought to contribute reward value processing, but a causal relationship has not been established. Here we use an inhibitory DREADD (Designer Receptor Exclusively Activated by Designer Drug) repeatedly and non-invasively inactivate rmCD macaque monkeys. We inject adeno-associated viral vector expressing the DREADD, hM4Di, into bilaterally. To visualize expression in vivo , develop non-invasive imaging method using positron emission...
Video-based markerless motion capture permits quantification of an animal's pose and motion, with a high spatiotemporal resolution in naturalistic context, is powerful tool for analyzing the relationship between behaviors its brain functions. Macaque monkeys are excellent non-human primate models, especially studying neuroscience. Due to lack dataset allowing training deep neural network macaque's it has been challenging apply this technology macaques-based studies. In study, we created...
The processing of stress responses involves brain-wide communication among cortical and subcortical regions; however, the underlying mechanisms remain elusive. Here, we show that claustrum (CLA) is crucial for control stress-induced anxiety-related behaviors. A combined approach using brain activation mapping machine learning showed CLA serves as a reliable marker exposure to acute stressors. In TRAP2 mice, which allow activity-dependent genetic labeling, chemogenetic neuronal ensemble...
Intracerebral vector delivery in nonhuman primates has been a major challenge. We report successful blood-brain barrier opening and focal of adeno-associated virus serotype 9 vectors into brain regions involved Parkinson’s disease using low-intensity focus ultrasound adult macaque monkeys. Openings were well tolerated with generally no associated abnormal magnetic resonance imaging signals. Neuronal green fluorescent protein expression was observed specifically confirmed opening. Similar...
Abstract Epilepsy is a disorder in which abnormal neuronal hyperexcitation causes several types of seizures. Because pharmacological and surgical treatments occasionally interfere with normal brain function, more focused on-demand approach desirable. Here we examined the efficacy chemogenetic tool—designer receptors exclusively activated by designer drugs (DREADDs)—for treating focal seizure nonhuman primate model. Acute infusion GABA A receptor antagonist bicuculline into forelimb region...
Recent emphasis has been placed on gene transduction mediated through recombinant adeno-associated virus (AAV) vector to manipulate activity of neurons and their circuitry in the primate brain. In present study, we created a novel which capsid was composed proteins derived from both AAV serotypes 1 2 (AAV1 AAV2). Following injection into frontal cortex macaque monkeys, this mosaic vector, termed AAV2.1 found exhibit excellence transgene expression (for AAV1 vector) neuron specificity AAV2...
Brain injuries such as stroke damage neural circuitry and lead to functional deficits. Spared motor pathways are often reorganized contribute recovery; however, the connectivity molecular mechanisms that drive reorganization largely unknown. Here, we demonstrate structural reformed by spared corticospinal axons after determine a key secretory protein drives reorganization. We first found innervate specific areas of denervated cervical cord stroke. Anatomical photometric analyses reveal...
Dual transneuronal tracing is a novel viral methodology which employs two recombinant viruses, each expressing different reporter protein. Peripheral injection of pseudorabies viruses has been used as powerful method to define neurons that coordinate outputs various peripheral targets motor and autonomic systems. Here, we assessed the feasibility recombinants rabies virus (RV) vector for dual in central nervous system. First, examined whether RV-vectors can double label cells vitro, showed...
Abstract Optogenetics enables temporally and spatially precise control of neuronal activity in vivo . One the key advantages optogenetics is that it can be used to targeted neural pathways connect specific brain regions. While such pathway-selective optogenetic a popular tool rodents, attempts at modulating behaviour using have not yet been successful primates. Here we develop methodology for macaque monkeys, focusing on pathway from frontal eye field (FEF) superior colliculus (SC), part...
The bottom-up processing of visual information is strongly influenced by top-down signals, at least part which thought to be conveyed from the frontal cortex through eye field (FEF) and lateral intraparietal area (LIP). Here we investigated architecture multisynaptic pathways middle temporal (MT) dorsal stream 4 (V4) ventral in macaques. In first series experiments, retrograde trans-synaptic tracer, rabies virus, was injected into MT or V4. Three days after injections, second-order...