A5073830095- Vestibular and auditory disorders
- Photoreceptor and optogenetics research
- Virus-based gene therapy research
- Ophthalmology and Eye Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Visual perception and processing mechanisms
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Single-cell and spatial transcriptomics
- Retinal Development and Disorders
- Advanced Fluorescence Microscopy Techniques
- Olfactory and Sensory Function Studies
- Molecular Communication and Nanonetworks
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- CCD and CMOS Imaging Sensors
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Muscle metabolism and nutrition
- Ion Transport and Channel Regulation
- Genetic Mapping and Diversity in Plants and Animals
- Herpesvirus Infections and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Adrenal and Paraganglionic Tumors
- Optical Coherence Tomography Applications
Duke University
2019-2025
University of Mississippi Medical Center
2012-2017
Jackson Memorial Hospital
2015
State Street (United States)
2014-2015
University of North Carolina at Greensboro
2008-2014
Cataloging the diverse cellular architecture of primate brain is crucial for understanding cognition, behavior, and disease in humans. Here, we generated a brain-wide single-cell multimodal molecular atlas rhesus macaque brain. Together, profiled 2.58 M transcriptomes 1.59 epigenomes from single nuclei sampled 30 regions across adult Cell composition differed extensively brain, revealing signatures region-specific functions. We also identified 1.19 candidate regulatory elements, many...
This paper experimentally examines different configurations of a multi-camera array microscope (MCAM) imaging technology. The MCAM is based upon densely packed “micro-cameras” to jointly image across large field-of-view (FOV) at high resolution. Each micro-camera within the images unique area sample interest, and then all acquired data with 54 micro-cameras are digitally combined into composite frames, whose total pixel counts significantly exceed standard systems. We present results from...
Understanding the neurobiological mechanisms underlying HIV-associated neurocognitive decline in people living with HIV is frequently complicated by an inability to analyze changes across course of infection and frequent presence comorbid psychiatric substance use disorders. Preclinical non-human primate simian immunodeficiency virus (SIV) models help address these shortcomings. However, SIV studies target protracted endpoints, limiting our understanding neuromolecular alterations during...
We recently demonstrated a bilateral projection to the supraoculomotor area from central mesencephalic reticular formation (cMRF), region implicated in horizontal gaze changes. C-group motoneurons, which supply multiply innervated fibers medial rectus muscle, are located within primate area, but their inputs and function poorly understood. Here, we tested whether motoneurons Macaca fascicularis monkeys receive direct cMRF input by injecting this portion of with anterograde tracers...
Recently, we established an adeno-associated virus (AAV9) capsid-promoter interaction that directly determined cell-specific gene expression across two synthetic promoters, Cbh and CBA, in the rat striatum. These studies not only expand this to include another promoter striatum but also establish AAV interactions nonhuman primate brain. When serotype 9 vectors were injected into striatum, minimal JetI drove green fluorescent protein (GFP) predominantly oligodendrocytes. However, similar our...
It is known that four common inbred mouse strains show defects of the forebrain commissures. The BALB/cJ strain has a low frequency abnormally small corpus callosum, whereas 129 have many animals with deficient callosum. I/LnJ and BTBR T+ tf/J never half almost all severely reduced size hippocampal commissure. Certain F1 hybrid crosses among these are to be less abnormal than parents, suggesting parent different genetic causes commissure defects. In this study, were investigated. × I/Ln...
ABSTRACT This study explores two points related to the pattern of innervation extraocular muscles. First, species differences exist in location motoneurons supplying multiply innervated fibers (MIFs) and singly (SIFs) eye MIF are located outside nuclei primates, but intermixed with SIF within rat nuclei. To test whether this difference is visual capacity frontal placement eyes, we injected retrograde tracers into medial rectus muscle cat, a highly nonprimate frontally placed eyes. Distal...
The organization of extraocular muscles (EOMs) and their motor nuclei was investigated in the mouse due to increased importance this model for oculomotor research. Mice showed a standard EOM pattern, although eyes are set at side head. They do have more prominent oblique muscles, whose insertion points differ from those frontal-eyed species. Retrograde tracers revealed that motoneuron layout aligns with general vertebrate plan respect laterality. departed some significant respects previously...
Reliable viral vector-mediated transgene expression in primate motoneurons would improve our ability to anatomically and physiologically interrogate motor systems. We therefore investigated the efficacy of replication defective, early region 1-deleted canine adenovirus type-2 (CAV-2) vectors for mediating fluorescent proteins into brainstem following craniofacial intramuscular injections four rhesus monkeys (Macaca mulatta). Vector were placed surgically identified isolated muscles. After a...
Abstract Since most gaze shifts are to targets that lie at a different distance from the viewer than current target, changes commonly require change in angle between eyes. As part of this response, lens curvature must also be adjusted with respect target by ciliary muscle. It has been suggested projections cerebellar fastigial and posterior interposed nuclei supraoculomotor area (SOA), which lies immediately dorsal oculomotor nucleus contains near response neurons, support behavior. However,...
A projection by the superior colliculus to supraoculomotor area (SOA) located dorsal oculomotor complex was first described in 1978. This projection's targets have yet be identified, although initial study suggested that vertical gaze motoneuron dendrites might receive this input. Defining tectal is complicated fact SOA contains a number of different cell populations. In present study, we used anterograde tracers characterize collicular axonal arbors and retrograde label prospective target...
Decisions often involve the consideration of multiple cues, each which may inform selection on basis learned probabilities. Our ability to use probabilistic inference for decisions is bounded by uncertainty and constraints such as time pressure. Previous work showed that when humans choose between visual objects in a multiple-cue, task, they cope with pressure discounting least informative an example satisficing or "good enough" decision-making. We tested two rhesus macaques (Macaca mulatta)...