A5021627864- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Optical Imaging and Spectroscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Image Processing Techniques and Applications
- Optical Coherence Tomography Applications
- Digital Holography and Microscopy
- Non-Invasive Vital Sign Monitoring
- Inflammatory Bowel Disease
- Molecular Biology Techniques and Applications
- Optical measurement and interference techniques
- Advanced X-ray Imaging Techniques
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Cellular Mechanics and Interactions
- Advanced Optical Sensing Technologies
- Immune Response and Inflammation
- AI in cancer detection
- Immune Cell Function and Interaction
- Orthopedic Infections and Treatments
- Spectroscopy and Chemometric Analyses
- 3D Printing in Biomedical Research
- Advanced MRI Techniques and Applications
- Helicobacter pylori-related gastroenterology studies
Duke University
2022-2025
Friedrich-Alexander-Universität Erlangen-Nürnberg
2019-2024
Universitätsklinikum Erlangen
2021-2023
Czech Academy of Sciences, Institute of Biotechnology
2021
Advanced Optical Technologies (United States)
2020
Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging living cells tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope label-free in vivo small animals via side-viewing needle objectives is presented. Minimal invasive at resolution performed colonoscopy mice surgical measures dyes as contrast agent. The colon mucosa imaged repeatedly same animal mouse model acute intestinal...
Objective Mucosal T cells play a major role in inflammatory bowel disease (IBD). However, their immunometabolism during intestinal inflammation is poorly understood. Due to its impact on cellular metabolism and proinflammatory immune cell function, we here focus the enzyme ATP citrate lyase (ACLY) mucosal relevance for IBD. Design ACLY expression immunometabolic colitogenic function were analysed from patients with IBD two experimental colitis models. Results was markedly expressed colon...
Immune cell activity is a major factor for disease progression in inflammatory bowel diseases (IBD). Classifying the type and functional state of immune cells therefore crucial clinical diagnostics IBD. Label-free optical technologies exploiting NADH FAD autofluorescence, such as multiphoton microscopy, have been used to describe tissue morphology healthy inflamed colon samples. Nevertheless, strategy identification single subtypes within yet be developed. This work aims initiate an...
We report tensorial tomographic Fourier ptychography (T2oFu), a nonscanning label-free microscopy method for simultaneous imaging of quantitative phase and anisotropic specimen information in 3D. Built upon ptychography, technique, T2oFu additionally highlights the vectorial nature light. The setup consists standard microscope equipped with an LED matrix, polarization generator, polarization-sensitive camera. Permittivity tensors samples are computationally recovered from polarized intensity...
This paper experimentally examines different configurations of a multi-camera array microscope (MCAM) imaging technology. The MCAM is based upon densely packed “micro-cameras” to jointly image across large field-of-view (FOV) at high resolution. Each micro-camera within the images unique area sample interest, and then all acquired data with 54 micro-cameras are digitally combined into composite frames, whose total pixel counts significantly exceed standard systems. We present results from...
Diffuse correlation spectroscopy (DCS) allows label-free, non-invasive investigation of microvascular dynamics deep within tissue, such as cerebral blood flow (CBF). However, the signal-to-noise ratio (SNR) in DCS limits its effective sensitivity adults, which depth to brain, through scalp and skull, is substantially larger than infants. Therefore, we aim increase SNR and, ultimately, CBF new techniques. We present an vivo demonstration parallelized (PDCS) measure muscular healthy adults....
Abstract Noninvasive optical imaging through dynamic scattering media has numerous important biomedical applications but still remains a challenging task. While standard diffuse methods measure absorption or fluorescent emission, it is also well‐established that the temporal correlation of scattered coherent light diffuses tissue much like intensity. Few works to date, however, have aimed experimentally and process such data demonstrate deep‐tissue video reconstruction decorrelation...
Abstract Group 3 innate lymphoid cells (ILC3s) are crucial mediators of immunity and epithelial barrier function during immune responses against extracellular bacteria. Here, we identify Interferon regulatory factor 1 (IRF-1), a transcription previously associated with type immunity, as an essential regulator intestinal ILC3 accumulation effector cytokine production. We demonstrate that IRF-1 is upregulated in the context infection enteropathogen Citrobacter rodentium its presence central...
Abstract Background Closing mucosal defects to reach healing is an important goal of therapy in inflammatory bowel disease (IBD). Among other cells, monocyte‐derived macrophages are centrally involved such intestinal wound healing. We had previously demonstrated that the anti‐α4β7 integrin antibody vedolizumab blocks recruitment non‐classical monocytes as biased progenitors gut and delays However, although for interpretation disappointing results recent phase III trials IBD, effects anti‐β7...
We present a multi-modal fiber array snapshot technique (M-FAST) based on an of 96 compact cameras placed behind primary objective lens and bundle array. Our is capable large-area, high-resolution, multi-channel video acquisition. The proposed design provides two key improvements to prior cascaded imaging system approaches: novel optical arrangement that accommodates the use planar camera arrays, new ability acquire image data M-FAST multi-modal, scalable can dual-channel fluorescence images...
Rationale: Structural remodeling or damage as a result of disease injury is often not evenly distributed throughout tissue but strongly depends on localization and extent damaging stimuli. Skeletal muscle mechanically active organ can express signs local even systemic myopathic damage, necrosis, repair. Conventionally, biopsies (patients) whole muscles (animal models) are sliced stained to assess structural alterations histologically. Three-dimensional information be obtained by applying...
Cold atmospheric plasma (CAP) is partially ionized gas near room temperature with previously reported antitumor effects. Despite extensive research and growing interest in this technology, active components molecular mechanisms of CAP are not fully understood to date. We used Raman spectroscopy colorimetric assays determine elevated nitrite nitrate levels after treatment a MiniFlatPlaster device. Previously, we demonstrated CAP-induced acidification. Cellular effects strong extracellular...
Cancer cells facilitate tumor growth by creating favorable micro-environments (TME), altering homeostasis and immune response in the extracellular matrix (ECM) of surrounding tissue. A potential factor that contributes to TME generation ECM remodeling is cytoskeleton-associated human death-associated protein kinase 1 (DAPK1). Increased cell motility de-adhesion (thus, promoting metastasis), as well upregulated plasminogen-signaling, are shown when functionally analyzing DAPK1 ko-related...
An analysis of the microscopic tissue morphology is essential for a diagnosis inflammatory bowel disease (IBD).1Bryant R.V. Burger D.C. Delo J. et al.Beyond endoscopic mucosal healing in UC: histological remission better predicts corticosteroid use and hospitalisation over 6 years follow-up.Gut. 2016; 65: 408-414Crossref PubMed Scopus (236) Google Scholar Multiphoton microscopy (MPM) an advanced optical technology label-free imaging native tissues2Zipfel W.R. Williams R.M. Webb W.W....
Deep learning (DL) shows notable success in biomedical studies. However, most DL algorithms work as black boxes, exclude experts, and need extensive data. This is especially problematic for fundamental research the laboratory, where often only small sparse data are available objective knowledge discovery rather than automation. Furthermore, basic usually hypothesis-driven prior (priors) exists. To address this, Self-Enhancing Multi-Photon Artificial Intelligence (SEMPAI) that designed...
Mechanical stresses are an environmental challenge virtually all tissues in the body exposed to and thus, of fundamental interest study cell reactions mechanobiology. Yet, unlike acute short-term mechanical stimulations, long-term or cyclic mechano-stimulation as experienced is difficult reproduce. Bioreactors designed control culture conditions, but still, there yet no technical solutions available merge bioreactor opto-biomechatronics technologies for stretch-applications simultaneous live...
Diffuse Correlation Spectroscopy (DCS) allows the label-free investigation of microvascular dynamics deep within living tissue. However, common implementations DCS are currently limited to measurement depths $\sim 1-1.5cm$, which can limit accuracy cerebral hemodynamics measurement. Here we present massively parallelized (pDCS) using novel single photon avalanche detector (SPAD) arrays with up 500x500 individual channels. The new SPAD array technology boost signal-to-noise ratio by a factor...
Diffuse Correlation Spectroscopy (DCS) allows the optical and label-free investigation of microvascular dynamics. Commonly, DCS is implemented with highly sensitive ultra fast single-photon avalanche diodes (SPAD) for blood flow measurements from around 1-1.5cm deep inside tissue (source detector separation 2.5-3 cm). In parallelized (pDCS), we use arrays multiple SPADs to boost signal-to-noise ratio by averaging many independent measurements. this study, explored capabilities an innovative,...