A5040667714- Neuroscience and Neuropharmacology Research
- Neurological disorders and treatments
- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Neurotransmitter Receptor Influence on Behavior
- Transcranial Magnetic Stimulation Studies
- Kidney Stones and Urolithiasis Treatments
- Neuroscience of respiration and sleep
- Neuropeptides and Animal Physiology
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Drug Transport and Resistance Mechanisms
- Bone health and osteoporosis research
- Nerve injury and regeneration
- Motor Control and Adaptation
- Receptor Mechanisms and Signaling
- Retinal Development and Disorders
- Botulinum Toxin and Related Neurological Disorders
- Virus-based gene therapy research
- Functional Brain Connectivity Studies
- Genetic Neurodegenerative Diseases
- Parathyroid Disorders and Treatments
- Drug Solubulity and Delivery Systems
- Advanced Drug Delivery Systems
Kyoto University
2016-2025
National Institutes for Quantum Science and Technology
2024-2025
Kansai Medical University
2024
Osaka University
2023-2024
Japan Science and Technology Agency
2003-2023
Inuyama Chuo Hospital
2011-2023
Japan Agency for Medical Research and Development
2016-2022
Institute of Primate Research
2010-2021
University College London
2018
National Hospital for Neurology and Neurosurgery
2018
Neurotransmission in the hippocampus is modulated variously through presynaptic metabotropic glutamate receptors (mGluRs). To establish precise localization of mGluRs rat hippocampus, we used subtype-specific antibodies for eight (mGluR1-mGluR8) immunohistochemistry combined with lesioning three major hippocampal pathways: perforant path, mossy fiber, and Schaffer collateral. Immunoreactivity group II (mGluR2) III (mGluR4a, mGluR7a, mGluR7b, mGluR8) was predominantly localized to elements,...
The subthalamic nucleus (STN) is a key structure for somatic motor control via the basal ganglia. In present study, we demonstrate that STN of macaque monkey has dual sets body part representations. Each two separate portions characterized with somatotopically arranged direct cortical inputs are derived from primary cortex (MI) and supplementary area (SMA). first set representations transformed MI to lateral STN, whereas second SMA medial STN. Intracortical microstimulation mapping was...
Abstract The purpose of this study was to examine the commonly available methods noninvasively assessing bone mineral status across three defined female populations their interrelationships, compare respective abilities reflect age- and menopause-related loss, discriminate osteoporotic fractures, classify patients diagnostically. A total 47 healthy premenopausal (age 33 ± 7 years), 41 postmenopausal 64 9 36 70 6 years) women were examined with following techniques: (1) quantitative computed...
Abstract Parkinson’s disease is characterized by degeneration of nigral dopaminergic neurons, leading to a wide variety psychomotor dysfunctions. Accumulated evidence suggests that abnormally synchronized oscillations in the basal ganglia contribute expression parkinsonian motor symptoms. However, mechanism generates abnormal dopamine‐depleted state remains poorly understood. We addressed this question examining neuronal activity two 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine‐treated...
It is widely accepted that dorsal striatum neurons participate in either the direct pathway (expressing dopamine D1 receptors) or indirect D2 receptors), controlling voluntary movements an antagonistically balancing manner. The D1- and D2-expressing are activated inactivated, respectively, by released from substantia nigra encoding reward expectation. However, little known about functional representation of motor information its modulation individual striatal constituting two pathways. In...
Abstract Premotor neurons sending their axons to the trigeminal motor nucleus were observed in cat by light and electron microscopy after labeling retrogradely or anterogradely with horseradish peroxidase (HRP). After HRP injection into nucleus, labeled seen most frequently parvocellular reticular formation bilaterally. Many also contralaterally intermediate zone at rostralmost levels of cervical cord its rostral extension caudalmost medulla oblongata. Additionally, some ipsilaterally...
The lentiviral vector system based on human immunodeficiency virus type 1 (HIV-1) is used extensively in gene therapy trials of neurological and neurodegenerative diseases. Retrograde axonal transport viral vectors offers a great advantage to the delivery genes into neuronal cell bodies that are situated regions distant from injection site. Pseudotyping HIV-1-based with selective variants rabies glycoprotein (RV-G) increases transfer via retrograde central nervous system. Because large-scale...
Abstract The rostromedial caudate (rmCD) of primates is thought to contribute reward value processing, but a causal relationship has not been established. Here we use an inhibitory DREADD (Designer Receptor Exclusively Activated by Designer Drug) repeatedly and non-invasively inactivate rmCD macaque monkeys. We inject adeno-associated viral vector expressing the DREADD, hM4Di, into bilaterally. To visualize expression in vivo , develop non-invasive imaging method using positron emission...
Video-based markerless motion capture permits quantification of an animal's pose and motion, with a high spatiotemporal resolution in naturalistic context, is powerful tool for analyzing the relationship between behaviors its brain functions. Macaque monkeys are excellent non-human primate models, especially studying neuroscience. Due to lack dataset allowing training deep neural network macaque's it has been challenging apply this technology macaques-based studies. In study, we created...
The processing of stress responses involves brain-wide communication among cortical and subcortical regions; however, the underlying mechanisms remain elusive. Here, we show that claustrum (CLA) is crucial for control stress-induced anxiety-related behaviors. A combined approach using brain activation mapping machine learning showed CLA serves as a reliable marker exposure to acute stressors. In TRAP2 mice, which allow activity-dependent genetic labeling, chemogenetic neuronal ensemble...
Intracerebral vector delivery in nonhuman primates has been a major challenge. We report successful blood-brain barrier opening and focal of adeno-associated virus serotype 9 vectors into brain regions involved Parkinson’s disease using low-intensity focus ultrasound adult macaque monkeys. Openings were well tolerated with generally no associated abnormal magnetic resonance imaging signals. Neuronal green fluorescent protein expression was observed specifically confirmed opening. Similar...
Abstract Epilepsy is a disorder in which abnormal neuronal hyperexcitation causes several types of seizures. Because pharmacological and surgical treatments occasionally interfere with normal brain function, more focused on-demand approach desirable. Here we examined the efficacy chemogenetic tool—designer receptors exclusively activated by designer drugs (DREADDs)—for treating focal seizure nonhuman primate model. Acute infusion GABA A receptor antagonist bicuculline into forelimb region...
Recent emphasis has been placed on gene transduction mediated through recombinant adeno-associated virus (AAV) vector to manipulate activity of neurons and their circuitry in the primate brain. In present study, we created a novel which capsid was composed proteins derived from both AAV serotypes 1 2 (AAV1 AAV2). Following injection into frontal cortex macaque monkeys, this mosaic vector, termed AAV2.1 found exhibit excellence transgene expression (for AAV1 vector) neuron specificity AAV2...
Brain injuries such as stroke damage neural circuitry and lead to functional deficits. Spared motor pathways are often reorganized contribute recovery; however, the connectivity molecular mechanisms that drive reorganization largely unknown. Here, we demonstrate structural reformed by spared corticospinal axons after determine a key secretory protein drives reorganization. We first found innervate specific areas of denervated cervical cord stroke. Anatomical photometric analyses reveal...