A5021482868- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Neural and Behavioral Psychology Studies
- Neurotransmitter Receptor Influence on Behavior
- Memory and Neural Mechanisms
- Functional Brain Connectivity Studies
- Receptor Mechanisms and Signaling
- Cancer, Stress, Anesthesia, and Immune Response
- Obsessive-Compulsive Spectrum Disorders
- Retinal Development and Disorders
- Neurological disorders and treatments
- EEG and Brain-Computer Interfaces
- Neuroscience and Neural Engineering
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Visual perception and processing mechanisms
- Stress Responses and Cortisol
- Immune Cell Function and Interaction
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Alzheimer's disease research and treatments
- CRISPR and Genetic Engineering
- Parkinson's Disease Mechanisms and Treatments
- Regulation of Appetite and Obesity
- Primate Behavior and Ecology
- Immunotherapy and Immune Responses
National Institutes for Quantum Science and Technology
2016-2025
The University of Tokyo
2020-2023
The Open University of Japan
2021
Kyoto Prefectural University of Medicine
1990-2020
NTL Institute for Applied Behavioral Science
2010
Imaging Center
2010
Oak Ridge National Laboratory
1973-1975
University of Tennessee at Knoxville
1975
Osaka University
1967
Abstract The rostromedial caudate (rmCD) of primates is thought to contribute reward value processing, but a causal relationship has not been established. Here we use an inhibitory DREADD (Designer Receptor Exclusively Activated by Designer Drug) repeatedly and non-invasively inactivate rmCD macaque monkeys. We inject adeno-associated viral vector expressing the DREADD, hM4Di, into bilaterally. To visualize expression in vivo , develop non-invasive imaging method using positron emission...
The ability to infer others' mental states is essential social interactions. This ability, critically evaluated by testing whether one attributes false beliefs (FBs) others, has been considered be uniquely hominid and accompany the activation of a distributed brain network. We challenge taxon specificity this identify causal locus introducing an anticipatory-looking FB paradigm combined with chemogenetic neuronal manipulation in macaque monkeys. find spontaneous gaze bias macaques implicitly...
Abstract Epilepsy is a disorder in which abnormal neuronal hyperexcitation causes several types of seizures. Because pharmacological and surgical treatments occasionally interfere with normal brain function, more focused on-demand approach desirable. Here we examined the efficacy chemogenetic tool—designer receptors exclusively activated by designer drugs (DREADDs)—for treating focal seizure nonhuman primate model. Acute infusion GABA A receptor antagonist bicuculline into forelimb region...
Recent emphasis has been placed on gene transduction mediated through recombinant adeno-associated virus (AAV) vector to manipulate activity of neurons and their circuitry in the primate brain. In present study, we created a novel which capsid was composed proteins derived from both AAV serotypes 1 2 (AAV1 AAV2). Following injection into frontal cortex macaque monkeys, this mosaic vector, termed AAV2.1 found exhibit excellence transgene expression (for AAV1 vector) neuron specificity AAV2...
Activity in several areas of the human brain and monkey increases when a subject anticipates events associated with reward, implicating role for bias decision action. However, real life, do not always appear as expected, we must choose an undesirable More than half neurons centromedian (CM) thalamus were selectively activated small-reward action was required but large-reward option anticipated. Electrical stimulation CM after request substituted brisk performance sluggish performance. These...
Discussion and SummaryThe present data show a dramatic age-related decrease in cell-mediated immunity as measured by vitro3H-dThd incorporation PHA-stimulated mouse spleen cells. Thus, not only is there marked decline humoral immune responsiveness during senescence (5), but it now appears that significant also occurs. It has been recently reported from this laboratory Peterson et al. (18), cells aged animals are less effective producing acute mortality the classical graft-versus-host...
Processing incentive and drive is essential for control of goal-directed behavior. The limbic part the basal ganglia has been emphasized in these processes, yet exact neuronal mechanism remained elusive. In this study, we examined activity ventral pallidum (VP) its upstream area, rostromedial caudate (rmCD), while two male macaque monkeys performed an instrumental lever release task which a visual cue indicated forthcoming reward size. We found that some neurons VP rmCD reflected expected...
Imaging-guided selective silencing of the prefronto-caudate/thalamus pathways reveals their distinct roles in cognitive functions.
The chemogenetic technology referred to as designer receptors exclusively activated by drugs (DREADDs) offers reversible means control neuronal activity for investigating its functional correlation with behavioral action. Deschloroclozapine (DCZ), a recently developed highly potent and selective DREADD actuator, displays capacity expand the utility of DREADDs chronic manipulation without side effects in nonhuman primates, which has not yet been validated. Here we investigated...
Serotonin (5-HT) deficiency is a core biological pathology underlying depression and other psychiatric disorders whose key symptoms include decreased motivation. However, the exact role of 5-HT in motivation remains controversial elusive. Here, we pharmacologically manipulated system macaque monkeys quantified effects on for goal-directed actions terms incentives costs. Reversible inhibition synthesis increased errors reaction times tasks, indicating reduced Analysis found...
Primates must adapt to changing environments by optimizing their behavior make beneficial choices. At the core of adaptive is orbitofrontal cortex (OFC) brain, which updates choice value through direct experience or knowledge-based inference. Here, we identify distinct neural circuitry underlying these two separate abilities. We designed behavioral tasks in male macaque monkeys updated values certain items, either directly experiencing changes stimulus-reward associations, inferring...
Abstract Visual object memory is a fundamental element of various cognitive abilities, and the underlying neural mechanisms have been extensively examined especially in anterior temporal cortex primates. However, both macroscopic large-scale functional network which this region embedded microscopic neuron-level dynamics top-down regulation it receives for remains elusive. Here, we identified orbitofrontal node as critical partner by combining whole-brain imaging during rest short-term task...
Decision making and action selection are influenced by the values of benefit, reward, cost, punishment. Mapping positive negative external events actions occurs mainly via discharge rates neurons in cerebral cortex, amygdala, basal ganglia. However, it remains unclear how reward encoded ganglia integrated into value-based control limb-movement through corticobasal loops. To address this issue, we investigated activities presumed projection putamen macaque monkeys performing a visually...
To interrogate particular neuronal pathways in nonhuman primates under natural and stress-free conditions, we applied designer receptors exclusively activated by drugs (DREADDs) technology to common marmosets. We injected adeno-associated virus vectors expressing the excitatory DREADD hM3Dq into unilateral substantia nigra (SN) four Using multi-tracer positron emission tomography imaging, detected expression vivo, which was confirmed nigrostriatal dopamine neurons immunohistochemistry, as...
Inhibitory interneurons are the fundamental constituents of neural circuits that organize network outputs. The striatum as part basal ganglia is involved in reward-directed behaviors. However, role inhibitory this process remains unclear, especially behaving monkeys. We recorded striatal single neuron activity while monkeys performed hand or eye movements. Presumed parvalbumin-containing GABAergic (fast-spiking neurons, FSNs) were identified based on narrow spike shapes three independent...
The orbitofrontal cortex (OFC) and its major downstream target within the basal ganglia—the rostromedial caudate nucleus (rmCD)—are involved in reward-value processing goal-directed behavior. However, a causal contribution of pathway linking these two structures to behavior has not been established. Using chemogenetic technology designer receptors exclusively activated by drugs with crossed inactivation design, we functionally reversibly disrupted interactions between OFC rmCD male macaque...
The ventral striatum (VS) is a key brain region for reward processing and motivation, its dysfunctions have been implicated in psychiatric disorders such as apathy obsessive-compulsive disorder. Although functional heterogeneity within the VS has well established rodents, relevance mechanisms primates remain unclear. To address this issue, we performed bilateral pharmacological inactivation of two male macaque monkeys using muscimol, GABA A receptor agonist. Precise targeting was achieved...
Most of our daily actions are selected and executed involuntarily under familiar situations by the guidance internal drives, such as motivation. The behavioral tendency or biasing towards one over others reflects action-selection process in advance action execution (i.e., pre-action bias). Facing unexpected situations, however, bias should be withdrawn replaced an alternative that is suitable for situation counteracting To understand neural mechanism process, we studied activity thalamic...
The term ‘temporal discounting’ describes both choice preferences and motivation for delayed rewards. Here we show that neuronal activity in the dorsal part of primate caudate head (dCDh) signals temporally discounted value needed to compute Macaque monkeys performed an instrumental task, which visual cues indicated forthcoming size delay duration before reward. Single dCDh neurons represented without reflecting changes animal’s physiological state. Bilateral pharmacological or chemogenetic...