A5062513710- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Genetics and Neurodevelopmental Disorders
- Neuroendocrine regulation and behavior
- Autism Spectrum Disorder Research
- Receptor Mechanisms and Signaling
- Asthma and respiratory diseases
- Stress Responses and Cortisol
- Pain Mechanisms and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Tryptophan and brain disorders
- Helicobacter pylori-related gastroenterology studies
- Bacterial Genetics and Biotechnology
- Advanced Radiotherapy Techniques
- Pluripotent Stem Cells Research
- Pesticide and Herbicide Environmental Studies
- Mitochondrial Function and Pathology
- Treatment of Major Depression
- Ion channel regulation and function
- Memory and Neural Mechanisms
- Neurotransmitter Receptor Influence on Behavior
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Allergic Rhinitis and Sensitization
- MicroRNA in disease regulation
Tokyo University of Agriculture
2021-2025
Bioscience (China)
2025
Niigata University
2025
Osaka University
2015-2024
National Center of Neurology and Psychiatry
2021-2023
Nagoya University
2022
Nagoya University Hospital
2022
National Institute of Mental Health
2021
Pharmac
2019-2020
Graco (United States)
2019
The N-methyl-d-aspartate (NMDA) receptors play critical roles in synaptic plasticity, neuronal development, and excitotoxicity. Tyrosine phosphorylation of NMDA by Src-family tyrosine kinases such as Fyn is implicated plasticity. To precisely address the receptor phosphorylation, we identified Fyn-mediated sites on GluR epsilon 2 (NR2B) subunit receptors. Seven out 25 residues C-terminal cytoplasmic region were phosphorylated vitro. Of these 7 residues, Tyr-1252, Tyr-1336, Tyr-1472 human...
Abstract The therapeutic mechanisms of lithium for treating bipolar mood disorder remain poorly understood. Recent studies demonstrate that has neuroprotective actions against a variety insults. Here, we studied effects excitotoxicity in cultured cerebral cortical neurons. Glutamate‐induced neurons was exclusively mediated by NMDA receptors. Pre‐treatment with LiCl time‐dependently suppressed maximal protection after 6 days pre‐treatment. Significant observed at the and subtherapeutic...
The urease of Helicobacter pylori has been proposed to be one its pathogenic factors. A kanamycin resistance determinant was inserted in a cloned gene, and transformation-mediated allelic exchange mutagenesis carried out introduce the disrupted gene into corresponding wild-type chromosomal region clinical isolate H. pylori, CPY3401. resulting mutant, HPT73, had null activity urease. Nude mouse stomachs were challenged with these two isogenic strains examine role pathogenesis. Gastritis found...
Metapyrocatechase which catalyzes the oxygenative ring cleavage of catechol to form a-hydroxymuconic Esemialdehyde is encoded by xylE gene on TOL plasmid Pseudomonas putida mt-2.We have cloned xytE region in Escherichia coli and determined nucleotide sequence DNA fragment 985 base pairs around gene.The included only one open translational frame sufficient length accommodate enzyme.The predicted amino acid consisted 307 residues, its NH2-and COOH-termind sequences were perfect agreement with...
Neural circuits are shaped by elimination of early-formed redundant synapses during postnatal development. Retrograde signaling from postsynaptic cells regulates synapse elimination. In this work, we identified semaphorins, a family versatile cell recognition molecules, as retrograde signals for climbing fiber to Purkinje in developing mouse cerebellum. Knockdown Sema3A, secreted semaphorin, or its receptor fibers accelerated day 8 (P8) P18. Conversely, knockdown Sema7A, membrane-anchored...
Three times crystallized metapyrocatechase has a specific activity of about 110 pmoles per mln mg protein at 24" and contains 1 g atom iron mole enzyme, based on molecular weight 140,000.The amino acid composition the enzyme is presented.Metapyrocatechase easily inactivated by various oxidizing agents, such as air or HzOz.This inactivation appears to be due oxidation ferrous ion ferric form, which then released from protein.The thus can fully reactivated incubation with reducing agent under...
Elimination of early-formed redundant synapses during postnatal development is essential for functional neural circuit formation. Purkinje cells (PCs) in the neonatal cerebellum are innervated by multiple climbing fibers (CFs). A single CF strengthened whereas other CFs eliminated each PC dependent on postsynaptic activity PC, but underlying mechanisms largely unknown. Here, we report that brain-derived neurotrophic factor (BDNF) from facilitates synapse elimination. By PC-specific deletion...
Autism spectrum disorder (ASD) is a complex group of clinically heterogeneous neurodevelopmental disorders with unclear etiology and pathogenesis. Genetic studies have identified numerous candidate genetic variants, including de novo mutated ASD-associated genes; however, the function these genes remains despite extensive bioinformatics resources. Accordingly, it not easy to assign priorities for further biological analysis. Here we developed convenient system identifying an experimental...
Abstract Pogo transposable element derived with ZNF domain ( POGZ ) has been identified as one of the most recurrently de novo mutated genes in patients neurodevelopmental disorders (NDDs), including autism spectrum disorder (ASD), intellectual disability and White-Sutton syndrome; however, neurobiological basis behind these remains unknown. Here, we show that regulates neuronal development ASD-related mutations impair developing mouse brain induced pluripotent cell lines from an ASD...
The processing of stress responses involves brain-wide communication among cortical and subcortical regions; however, the underlying mechanisms remain elusive. Here, we show that claustrum (CLA) is crucial for control stress-induced anxiety-related behaviors. A combined approach using brain activation mapping machine learning showed CLA serves as a reliable marker exposure to acute stressors. In TRAP2 mice, which allow activity-dependent genetic labeling, chemogenetic neuronal ensemble...
Abstract Chronic social isolation increases the risk of mental health problems, including cognitive impairments and depression. While subanesthetic ketamine is considered effective for in patients with depression, neural mechanisms underlying its effects are not well understood. Here we identified unique activation anterior insular cortex (aIC) as a characteristic feature brain-wide regions mice reared treated ( R )-ketamine, enantiomer. Using fiber photometry recording on freely moving...
Abstract Despite abundant evidence implicating the importance of N ‐methyl‐ d ‐aspartate (NMDA) receptors in spinal cord for pain transmission, signal transduction coupled to NMDA receptor activation is largely unknown neuropathic state that lasts over periods weeks. To address this, we prepared mice with by transection nerve L5. Wild‐type, NR2A‐deficient, and NR2D‐deficient developed pain; addition, phosphorylation NR2B subunits at Tyr1472 was observed superficial dorsal horn 1 week after...
We have determined the nucleotide sequence of structural gene for colicin E1, which consists 1,566 base pairs. The amino acid (522 residues) protein was derived from DNA sequence, and molecular weight calculated to be 57,279. From analysis predicted secondary structure, there appear three consecutive long alpha-helices in NH2-terminal half polypeptide, spanning 40, 100, 35 residues. In addition, is a polypeptide region near COOH terminus that shows homology signal portions outer membrane...
The regulatory gene xylS on the TOL plasmid of Pseudomonas putida activates transcription xylDLEGF operon for m-toluate-degrading pathway in presence m-toluate. also same m-xylene or m-methylbenzyl alcohol, but this activation another gene, xylR, is required. In study we examined expression by determining mRNA reverse transcriptase mapping and monitoring enzyme activity xylE product, which was expressed under control promoter. results above experiments provide evidence that xylR positively...
The regulatory gene xylR of the TOL plasmid, which functions positively on both xylABC and xylDEGF operons in presence m-xylene or m-methylbenzyl alcohol, was cloned onto an Escherichia coli vector, pACYC177. A fused operon consisting operator-promoter region xylE pBR322. product, catechol 2,3-dioxygenase, induced by alcohol cells containing when a 2.8-kilobase segment plasmid provided trans. Therefore, appeared to contain xylR. mapped very close other gene, xylS, determined previously. not...
The xylDEGF operon and the regulatory gene xylS of TOL plasmid found in Pseudomonas putida mt-2 were cloned onto Escherichia coli vector plasmids. A 9.5-kilobase fragment, derived from segment pTN2 deoxyribonucleic acid, carried xyl genes D, E, G, F, which encode toluate oxygenase, catechol 2,3-oxygenase, 2-hydroxymuconic semialdehyde dehydrogenase, hydrolase, respectively. enzymes noninducible unless a 3-kilobase PstI also segment, was provided either cis or trans. fragment appeared to...
Cleavage sites for the restriction endonucleases XhoI, BamHI, HindIII, and EcoRI were mapped on pTN2 plasmid, a recombinant of TOL RP4, which specifies toluene-degrading enzymes in same way as wild-type plasmid. The purified from strain Escherichia coli, contained entire length RP4 plasmid (about 54 kilobase pairs [kb]) segment 56 kb). is inserted at about 12 5 kb away BamHI cleavage respectively. also an insertion mutant, pTN1, two deletion mutants, pTN81 pTN9. Analysis pTN9 allowed...
Leptin acts in the hippocampus to enhance cognition and reduce depression anxiety. Cognitive emotional disorders are associated with abnormal hippocampal dendritic spine formation synaptogenesis. Although leptin has been shown induce synaptogenesis hypothalamus, its effects on mechanism(s) involved not well understood. Here we show that receptors (LepRs) critical for vivo because db/db mice lacking long form of receptor (LepRb) have reduced density CA1 CA3 neurons. promotes mature spines...