A5074161462- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Microbial metabolism and enzyme function
- Fermentation and Sensory Analysis
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- earthquake and tectonic studies
- Seismology and Earthquake Studies
- Amino Acid Enzymes and Metabolism
- Advanced ceramic materials synthesis
- Earthquake Detection and Analysis
- Plant Physiology and Cultivation Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Photosynthetic Processes and Mechanisms
- Catalysis for Biomass Conversion
- Genomics and Phylogenetic Studies
- Microbial Metabolites in Food Biotechnology
- Botany and Plant Ecology Studies
- Biochemical and Molecular Research
- Horticultural and Viticultural Research
- Microbial Fuel Cells and Bioremediation
- Metallurgical Processes and Thermodynamics
Yamaguchi University
2016-2025
Waseda University
2005-2022
Alzahra University
2022
Science Council of Japan
2017-2022
Kyoto University
2011
Gifu University
2006-2009
Khon Kaen University
2007
Osaka University
2005
Kansai University
2005
Obayashi (Japan)
2000-2003
Kombucha, one of the ordinary fermented beverages consumed worldwide, is produced by fermenting tea and sugar with a symbiotic culture bacteria yeasts or so-called SCOBY. Kombucha can be made from different types tea, such as black, green, white, red, oolong teas, yielding various health benefits properties. Several species are involved in fermentation process, which generates many beneficial compounds, polyphenols, organic acids, amino vitamins, minerals, nitrogens, hydrolytic enzymes, have...
We demonstrate herein the ability of Kluyveromyces marxianus to be an efficient ethanol producer and host for expressing heterologous proteins as alternative Saccharomyces cerevisiae. Growth production by strains K. S. cerevisiae were compared under same conditions. DMKU3-1042 was found most suitable strain high-temperature growth at 45 degrees C. This strain, but not cerevisiae, utilized cellobiose, xylose, xylitol, arabinose, glycerol, lactose. To develop a derivative genetic engineering,...
High-temperature fermentation technology with thermotolerant microbes has been expected to reduce the cost of bioconversion cellulosic biomass fuels or chemicals. Thermotolerant Kluyveromyces marxianus possesses intrinsic abilities ferment and assimilate a wide variety substrates including xylose efficiently produce proteins. These capabilities have found exceed those traditional ethanol producer Saccharomyces cerevisiae lignocellulose-bioconvertible ethanologenic Scheffersomyces stipitis....
Abstract Several raw materials have been used as partial supplements or entire replacements for the main ingredients of kombucha to improve biological properties resulting beverage. This study pineapple peels and cores (PPC), byproducts processing, alternative instead sugar production. Kombuchas were produced from fusions black tea PPC at different ratios, their chemical profiles properties, including antioxidant antimicrobial activities, determined compared with control without...
The mechanism of the enhanced bactericidal action to Escherichia coli lysozyme having a hydrophobic pentapeptide (Phe-Phe-Val-Ala-Pro) at its C terminus was investigated. modified lysozyme, pentapeptide-fused (HLz), secreted in culture medium from yeast harboring expression plasmid, which synthetic DNA fragment encoding introduced 3'-end coding region cDNA. Although CD analysis showed that HLZ considerably different wild-type (WLz) secondary and tertiary structures, it retained 76% lytic...
We have determined the nucleotide sequence of structural gene for colicin E1, which consists 1,566 base pairs. The amino acid (522 residues) protein was derived from DNA sequence, and molecular weight calculated to be 57,279. From analysis predicted secondary structure, there appear three consecutive long alpha-helices in NH2-terminal half polypeptide, spanning 40, 100, 35 residues. In addition, is a polypeptide region near COOH terminus that shows homology signal portions outer membrane...
The molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli was investigated. Genome-wide screening with single-gene knockout library provided list of genes indispensable for growth 47°C, called thermotolerant genes. Genes which expression affected by exposure to CHT were identified DNA chip analysis. Unexpectedly, the former contents did not overlap latter except dnaJ and dnaK, indicating that specific set non-heat shock is required organism survive...
The complete nucleotide sequence of the glucitol (gut) operon in Escherichia coli has been determined.The glucitol-specific Enzyme I1 and I11 phos-phoeno1pyruvate:sugar phosphotransferase system as well glucitol-6-phosphate dehydrogenase which are encoded by gutA, gutB, gutD genes gut operon, respectively, predicted to consist 506 (M, = 54,018), 123 (Mr 13,306), 259 27,866) amino acyl residues, respectively.The hydropathic profile I F revealed 7 or 8 long hydrophobic segments may traverse...
The cloning of two novel genes that encode cytokinin oxidase/dehydrogenase (CKX) in barley is described this work. Transformation both into Arabidopsis and tobacco showed at least one the codes for a functional enzyme, as its expression caused cytokinin-deficient phenotype heterologous host plants. Additional gene fragments, an silico search public expressed sequence tag clone databases, revealed presence 13 more members CKX family wheat. three selected was analyzed by RT-PCR found to be...
Adenylate kinase (AE) is known to play an important role in homeostasis of adenine nucleotide metabolism. We isolated cDNAs for rat AK isozymes (AKl, AK2, and AK3), determined their mRNAs tissues by Northern blot analysis, measured the isozyme activities. Tissue-dependent activities AKl AK2 paralleled contents mRNAs. Tissues with high AE1 levels showed low rice versa, suggesting that tissue-specific expressions genes are inversely regulated. AK3 mRNA was detected most examined, gene...
Ethanol production at elevated temperatures requires high potential thermotolerant ethanol-producing yeast. In this study, nine isolates of yeasts capable growth and ethanol were successfully isolated. Among these isolates, the newly isolated yeast strain, which was designated as Saccharomyces cerevisiae DBKKU Y-53, exhibited great for from sweet sorghum juice (SSJ) temperatures. The maximum concentrations produced by 37 °C 40 under optimum cultural condition 106.82 g·L−1 85.01 g·L−1,...
Efficient bioconversion of lignocellulosic biomass to bioethanol is one key challenges in the situation increasing demand. The ethanologenic microbes for such conversion are required possess abilities utilization various sugars including xylose and arabinose biomass. As additional characteristics, there a weak or no glucose repression that allows cells simultaneously utilize together with thermotolerance fermentation at high temperatures, which has several advantages reduction cooling cost....
Abstract High-temperature ethanol fermentation by thermotolerant yeast is considered a promising technology for production, especially in tropical and subtropical regions. In this study, optimization conditions high-temperature of pineapple waste hydrolysate (PWH) using newly isolated yeast, Saccharomyces cerevisiae HG1.1, the expression genes during at 40 °C were carried out. Three independent variables, including cell concentration, pH, extract, positively affected production from PWH °C....
Abstract Byproducts from the sugarcane manufacturing process, specifically molasses (SM) and bagasse (SB), can be used as alternative raw materials for sorbitol production via biological fermentation process. This study investigated of SM hydrolysate (SBH) using a thermally adapted Zymomonas mobilis ZM AD41. Various combinations SBH on batch process were tested. The results revealed that alone (FM1) or mixture at ratio 3:1 (FM2) based sugar mass in material proved to best condition by AD41...
Abstract Background Spathaspora passalidarum is a yeast with the highly effective capability of fermenting several monosaccharides in lignocellulosic hydrolysates, especially xylose. However, this was shown to be sensitive furfural released during pretreatment and hydrolysis processes lignocellulose biomass. We aimed improve tolerance previously isolated S. CMUWF1−2, which presented thermotolerance no detectable glucose repression, via adaptive laboratory evolution (ALE). Results An adapted...