A5017593738- Endometrial and Cervical Cancer Treatments
- Ovarian cancer diagnosis and treatment
- Reproductive System and Pregnancy
- Cytomegalovirus and herpesvirus research
- Cervical Cancer and HPV Research
- Ectopic Pregnancy Diagnosis and Management
- Parvovirus B19 Infection Studies
- Endometriosis Research and Treatment
- Metabolism, Diabetes, and Cancer
- Pregnancy and preeclampsia studies
- Blood Coagulation and Thrombosis Mechanisms
- Uterine Myomas and Treatments
- Pregnancy-related medical research
- Systemic Lupus Erythematosus Research
- Cardiovascular Issues in Pregnancy
- Management of metastatic bone disease
- Radiomics and Machine Learning in Medical Imaging
- Preterm Birth and Chorioamnionitis
- Protein Kinase Regulation and GTPase Signaling
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Diabetes and associated disorders
- Maternal and fetal healthcare
- Herpesvirus Infections and Treatments
- Bacterial Genetics and Biotechnology
Sapporo University
2022-2025
Hokkaido University
2006-2025
Health Sciences University of Hokkaido
2021-2024
Tokai University
2021
Kobe University
2011-2020
Sapporo City General Hospital
2020
Hyogo Prefectural Cancer Center
2013-2014
Kobe University Hospital
2014
Kanazawa Medical University
2013
Tokushima University
1990-2011
To test whether the tyrosine kinase activity of insulin receptor is crucial for action, we have constructed mutations human at Lys-1030, which in presumed ATP-binding region. By using oligonucleotide-directed mutagenesis, this lysine residue was replaced with either methionine, arginine, or alanine. Chinese hamster ovary cells were transfected by mutant cDNAs and expressed receptors characterized. We show here that none these mutants exhibited insulin-activated autophosphorylation vitro....
Metapyrocatechase which catalyzes the oxygenative ring cleavage of catechol to form a-hydroxymuconic Esemialdehyde is encoded by xylE gene on TOL plasmid Pseudomonas putida mt-2.We have cloned xytE region in Escherichia coli and determined nucleotide sequence DNA fragment 985 base pairs around gene.The included only one open translational frame sufficient length accommodate enzyme.The predicted amino acid consisted 307 residues, its NH2-and COOH-termind sequences were perfect agreement with...
The transformation of human cells was examined by transfection cloned oncogenic DNAs derived from the tumor virus simian 40 and bladder carcinoma cell line EJ into diploid fibroblasts foreskin (FS-2 cells). DNA found to induce a morphologically transformed phenotype, leading easily detectable focus formation. Tumor antigen produced, but were not tumorigenic in nude mouse. gene, mutant form cellular c-Ha-ras actively transforms NIH/3T3 mouse CHEF/18 hamster is inactive FS-2 cells....
Physical exercise induces translocation of GLUT4 from an intracellular pool to the cell surface in skeletal muscles and increases glucose uptake via insulin-independent pathway. However, molecular mechanism remains be identified. Some studies have suggested that bradykinin is locally released contracting may responsible for increase transport muscles. To determine whether directly triggers translocation, we established L6 myotubes, 3T3-L1 adipocytes, Chinese hamster ovary cells stably...
The type 1 glucose transporter (GLUT1) gene encodes an integral membrane glycoprotein responsible for facilitating transfer of across plasma and is rapidly activated by serum, growth factors, oncogenic transformation. To elucidate the molecular mechanisms regulation GLUT1 expression, we isolated characterized mouse gene. DNA elements regulating transcription were analyzed in transient expression assays after transfection NIH/3T3 cells with a low background chloramphenicol acetyltransferase...
We have determined the nucleotide sequence of structural gene for colicin E1, which consists 1,566 base pairs. The amino acid (522 residues) protein was derived from DNA sequence, and molecular weight calculated to be 57,279. From analysis predicted secondary structure, there appear three consecutive long alpha-helices in NH2-terminal half polypeptide, spanning 40, 100, 35 residues. In addition, is a polypeptide region near COOH terminus that shows homology signal portions outer membrane...
LexA protein is a represssor of several chromosomal genes involved in the SOS response Escherichia coli.In previous experiments, we found that may also be repressor colicin E l gene.We now present evidence purified strongly repressed vitro transcription gene.As determined DNase I protection bound with high affinity to approximately 40-base pair long sequence between Pribnow box and start codon gene.The binding site was composed two overlapped "SOS boxes" which cooperative manner. ~~...
Abstract Recombinant clones containing the promoter region of human insulin receptor gene were isolated from genomic libraries derived nondiabetic persons. A 1.5-kilobase pair fragment 5'-flanking was sequenced. One transcriptional start site, located at 203 bases upstream translation identified by nuclease S1 mapping and primer extension experiment using mRNA. The bacterial chloramphenicol acetyltransferase assay revealed that a 573-base immediately preceding ATG has activity transcript...
The xylABC operon on the TOL plasmid directs synthesis of enzymes for conversion toluene to benzoate and is positively controlled by regulatory gene xylR. In study here nucleotide sequence was determined region this operon. in vivo transcription initiation site S1 nuclease reverse transcriptase mapping. RNA prepared from m-methylbenzyl alcohol-induced cells Pseudomonas putida Escherichia coli carrying pTN2, a derivative containing structural genes entire toluene-degrading pathway. amount E....
To examine the incidence and prognostic effects of intraoperative capsule rupture to assess effectiveness postoperative chemotherapy for tumor in apparent stage I epithelial ovarian cancer.This is a society-based retrospective observational study Japan that examined 15,163 women with IA-IC1 cancer who underwent primary surgical treatment between 2002 2015. Associations cause-specific survival, survival among intraoperatively ruptured cases were by histology type (clear cell n=6,107,...
We have analyzed the initiation and termination sites of transcription in vivo colicin E l gene Escherichia coli cells by S1-mapping assay RNA blot hybridization.According to assay, was initiated at about 75 base pairs upstream from NHS-terminal codon gene.The site corresponded with one two promoters which were previously determined vitro experiments (Ebina, Y., Kishi, F., Miki, T., Kagamiyama, H., Nakazawa, A. (1981) Gene 15, 119-126).Transcription stimulated cyclic AMP adenylate...
We obtained genetic and molecular evidence of non-thermosensitive recombinants RP4 (Kmr Tcr Cbr/Apr) the thermosensitive TOL plasmid. As first isolated in Pseudomonas aeruginosa PAO, recombinant plasmid pTN1 specified noninducible synthesis enzymes was transmissible to Escherichia coli on selection for transfer kanamycin resistance. The phenotypic expression genes E. low also noninducible. A spontaneous segregant, pTN2, appearing from pTN1, conferred inducible enzymes. These plasmids carry...
The aim of this prospective cohort study was to evaluate the efficacy maternal screening for congenital cytomegalovirus infection (CCI) using (CMV) immunoglobulin G (IgG) and IgG avidity index (AI).Pregnant women underwent CMV AI measurements. IgG-negative remeasurement after educational intervention. Women with an ≤45% received further examinations, including measurement IgM. All newborns polymerase chain reaction analyses urine, CCI diagnosed by detection CMV-DNA in urine. Primary defined...
Abstract Aim To examine occupational stress levels and associated factors among pregnant nurses through a comparison with non‐nurses. Methods This cross‐sectional study included 1060 working women. Participants completed questionnaires including the Brief Job Stress Questionnaire, work conditions, obstetric Sense of Coherence Scale, self‐management behaviors questionnaire. The participants were categorized into four groups: nurses, other healthcare workers, physical desk workers. Comparisons...
Fragments of 5'-flanking sequences the human insulin receptor gene were analyzed in transient expression assays after transfection cell lines with an improved low background chloramphenicol acetyl-transferase vector system pSVOOCAT (Araki, E., Shimada, F., Shichiri, M., Mori, and Ebina, Y. (1988) Nucleic Acids Res. 16, 1627). Transfection chimeric acetyltransferase plasmids containing various deletions insertions promoter HIR into CHO COS cells indicated that region between -629 -1...