A5085386025- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Neurobiology and Insect Physiology Research
- Molecular Communication and Nanonetworks
- Insect and Arachnid Ecology and Behavior
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Plant and animal studies
- Optical measurement and interference techniques
- Advanced Vision and Imaging
- Molecular spectroscopy and chirality
- Retinal Development and Disorders
- Photosynthetic Processes and Mechanisms
- Photochromic and Fluorescence Chemistry
- Biofield Effects and Biophysics
- Advanced Fluorescence Microscopy Techniques
- Gene Regulatory Network Analysis
- Animal Behavior and Reproduction
- Image Processing Techniques and Applications
- Plant and Biological Electrophysiology Studies
- Advanced Memory and Neural Computing
- CCD and CMOS Imaging Sensors
Janelia Research Campus
2016-2025
Howard Hughes Medical Institute
2016-2025
Helix (United States)
2016-2022
McGovern Institute for Brain Research
2011-2019
Massachusetts Institute of Technology
2011-2019
Technologies for silencing the electrical activity of genetically targeted neurons in brain are important assessing contribution specific cell types and pathways toward behaviors pathologies. Recently we found that archaerhodopsin-3 from Halorubrum sodomense (Arch), a light-driven outward proton pump, when expressed neurons, enables them to be powerfully, transiently, repeatedly silenced response pulses light. Because impressive characteristics Arch, explored optogenetic utility opsins with...
Topographic maps, the systematic spatial ordering of neurons by response tuning, are common across species. In Drosophila, lobula columnar (LC) neuron types project from optic lobe to central brain, where each forms a glomerulus in distinct position. However, advantages this glomerular arrangement unclear. Here, we examine functional and relationships 10 glomeruli using single-neuron calcium imaging. We discover novel detectors for objects smaller than lens resolution (LC18) complex line...
Vision provides animals with detailed information about their surroundings, conveying diverse features such as color, form, and movement across the visual scene. Computing these parallel spatial requires a large network of neurons, that in distant flies humans, regions comprise half brain's volume. These brain often reveal remarkable structure-function relationships, neurons organized along maps shapes directly relate to roles processing. To unravel stunning diversity complex system, careful...
Optogenetic neuronal network manipulation promises to unravel a long-standing mystery in neuroscience: how does microcircuit activity relate causally behavioral and pathological states? The challenge evoke spikes with high spatial temporal complexity necessitates further joint development of light-delivery approaches custom opsins. Two-photon (2P) light-targeting strategies demonstrated in-depth generation action potentials photosensitive neurons both vitro vivo , but thus far lack the...
Abstract Animals are often bombarded with visual information and must prioritize specific features based on their current needs. The neuronal circuits that detect relay have been well-studied. Yet, much less is known about how an animal adjusts its attention as goals or environmental conditions change. During social behaviors, flies need to focus nearby flies. Here, we study the flow of altered when female Drosophila enter aggressive state. From connectome, identified three state-dependent...
Abstract Vision provides animals with detailed information about their surroundings and conveys diverse features such as colour, form movement across the visual scene. Computing these parallel spatial requires a large network of neurons. Consequently, from flies to humans, regions in brain constitute half its volume. These often have marked structure–function relationships, neurons organized along maps shapes that directly relate roles processing. More than century anatomical studies...
Abstract Optogenetics provides a means to dissect the organization and function of neural circuits. also offers translational promise restoring sensation, enabling movement or supplanting abnormal activity patterns in pathological brain However, inherent sluggishness evoked photocurrents conventional channelrhodopsins has hampered development optoprostheses that adequately mimic rate timing natural spike patterning. Here, we explore feasibility limitations central auditory optoprosthesis by...
Channelrhodopsins (ChRs) are light-gated ion channels in widespread use neuroscience for mediating the genetically targetable optical control of neurons (optogenetics). ChRs pass multiple kinds ions, and although nonspecific ChR-mediated conductance is not an issue many studies, calcium protons, which can mediate diverse cellular signals, may be undesirable some instances. Here, we turned our attention to creation that have high cation photocurrent but fewer ions protons. We developed...
Abstract Animals are often bombarded with visual information and must prioritize specific features based on their current needs. The neuronal circuits that detect relay have been well studied 1–8 . Much less is known about how an animal adjusts its attention as goals or environmental conditions change. During social behaviours, flies need to focus nearby 9–11 Here we study the flow of altered when female Drosophila enter aggressive state. From connectome, identify three state-dependent...
ABSTRACT Optogenetic neuronal network manipulation promises to at last unravel a long-standing mystery in neuroscience: how does microcircuit activity causally relate behavioral and pathological states? The challenge evoke spikes with high spatial temporal complexity necessitates further joint development of light-delivery approaches custom opsins. Two-photon scanning parallel illumination strategies applied ChR2- C1V1-expressing neurons demonstrated reliable, in-depth generation action...