A5062313222- Neural dynamics and brain function
- Visual perception and processing mechanisms
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Advanced Chemical Sensor Technologies
- Antimicrobial agents and applications
- SARS-CoV-2 detection and testing
- Photoreceptor and optogenetics research
- Functional Brain Connectivity Studies
- EEG and Brain-Computer Interfaces
- Retinal Development and Disorders
- Face Recognition and Perception
- Dental Research and COVID-19
- Fluid Dynamics and Thin Films
- Nanomaterials and Printing Technologies
- Cognitive Science and Education Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Image Enhancement Techniques
- CCD and CMOS Imaging Sensors
- Epilepsy research and treatment
- Photochromic and Fluorescence Chemistry
- Sleep and Wakefulness Research
- Neuroscience, Education and Cognitive Function
- Nanofabrication and Lithography Techniques
- Optical Imaging and Spectroscopy Techniques
Monash University
2019-2024
Australian Regenerative Medicine Institute
2019-2024
Laboratoire Printemps
2023
Discovery Institute
2023
University College London
2015-2019
Institute of Ophthalmology
2016-2019
RIKEN Center for Brain Science
2010-2017
The University of Tokyo
2010-2013
Kansai University
2012
Tokyo University of the Arts
2011
Genetically encoded voltage indicators (GEVIs) promise to reveal the membrane potential of genetically targeted neuronal populations through noninvasive, chronic imaging large portions cortical space. Here we test a promising GEVI in mouse cortex during wakefulness, challenging condition due hemodynamic activity, and introduce straightforward projection method separate signal dominated by from activity. We expressed VSFP-Butterfly 1.2 plasmid layer 2/3 pyramidal cells visual electroporation...
Changes in arousal modulate the activity of mouse sensory cortex, but studies different mice and areas disagree on whether this modulation enhances or suppresses activity. We measured simultaneously multiple cortical by imaging expressing voltage-sensitive fluorescent proteins (VSFP). VSFP estimates local membrane potential across large portions cortex. used temporal filters to predict from running speed pupil dilation, two measures arousal. The provided good fits revealed that effects...
In the absence of external stimuli or overt behavior, activity left and right cortical hemispheres shows fluctuations that are largely bilateral. Here, we show these responsible for variability observed in responses to sensory stimuli. Using widefield imaging voltage calcium signals, measured cortex mice performing a visual detection task. Bilateral invested all areas, particularly those closest midline. Activity was less bilateral monocular region primary and, especially during task...
We prepared silica–poly(vinylpyrrolidone) (PVP) hybrid films via a temperature-controlled dip-coating process and investigated the influence of coating temperature (i.e., substrates, solutions atmosphere) on spontaneous pattern formation based "coffee-ring effect". Micron-scale stripe patterns were spontaneously formed surface at high temperatures over 40 °C during dip-coating. The arranged perpendicular to withdrawal direction, height width increased with increasing temperature. Such is...
Slow cortical waves that propagate across the cerebral cortex forming large-scale spatiotemporal propagation patterns are a hallmark of non-REM sleep and anaesthesia, but also occur during resting wakefulness. To investigate how spatial temporal properties slow change with depth anaesthetic, we optically imaged population voltage transients generated by mouse layer 2/3 pyramidal neurons one or two hemispheres dorsally genetically encoded indicator. From deep barbiturate anaesthesia to light...
It has been discussed that neural phase-synchrony across distant cortical areas (or global phase-synchrony) was correlated with various aspects of consciousness. The generating process the synchrony, however, remains largely unknown. As a first step, we investigate transient phase-synchrony, focusing on phase-synchronized clusters. We hypothesize clusters are dynamically organized before synchrony and clustering patterns depend perceptual conditions.In an EEG study, Kitajo reported...
Accurate definition of the borders cortical visual areas is essential for study neuronal processes leading to perception. However, data used areal boundaries have suffered from issues related resolution, uniform coverage, or suitability objective analysis, ambiguity. Here, we present a novel approach that combines widefield optical imaging, presentation naturalistic movies, and encoding model objectively define in primate extrastriate cortex. We applied this method test conflicting...
Despite the widespread application of general anesthesia for surgical procedures since 19th century, a fundamental understanding its operating principle and reliable metric Loss Consciousness (LoC) remain elusive, leaving unmitigated risks such as overdose or intraoperative awareness. Given that LoC is inducible by same anesthetics propofol in diverse animal species, here we propose there are corresponding shared neuronal mechanisms underlie LoC, which manifest common signatures neural...
The novel sudden acute respiratory syndrome coronavirus 2 is an enveloped virus currently causing severe illness and death worldwide. Common antiseptics such as alcohol have some efficacy in disinfecting everyday surroundings, but development of more effective disinfectants imperative. A series studies focusing on cationic antimicrobials resulted the a safe disinfectant, DEA-171, which provides ≥99.98 % inhibition all variants within 1 min.
Abstract Accurate definition of the borders cortical visual areas is essential for study neuronal processes leading to perception. However, data used areal boundaries has suffered from issues related resolution, uniform coverage, or suitability objective analysis, ambiguity. Here, we present a novel approach that combines widefield optical imaging, presentation naturalistic movies, and encoding model objectively define in primate extrastriate cortex. We applied this method test conflicting...
Abstract The ongoing activity of the left and right cortical hemispheres shows strong bilateral fluctuations. Here we show that these fluctuations are largely responsible for variability observed in responses to sensory stimuli. Using widefield imaging voltage calcium signals, measured cortex mice performing a visual detection task. Bilateral invested all areas, particularly those closest midline. Activity was less monocular region primary and, especially during task engagement, secondary...
We propose an extended framework of two dimensional neural field with network between distant cortical areas as a model global brain dynamics, and the models whose geometry changes depending on dynamics for growing brains. As characteristic pattern non-local interactions in field, pulser memory are constructed. Possible applications to quantitative measurements activities mouse human development briefly discussed.
The novel sudden acute respiratory syndrome coronavirus 2 is an enveloped virus currently causing severe illness and death worldwide. Common antiseptics such as alcohol have some efficacy in disinfecting everyday surroundings, but development of more effective disinfectants imperative. A series studies focusing on cationic antimicrobials resulted the a safe disinfectant, DEA-171, which provides ≥99.98% inhibition all variants within 1 min.
The novel sudden acute respiratory syndrome coronavirus 2 is an enveloped virus currently causing severe illness and death worldwide. Common antiseptics such as alcohol have some efficacy in disinfecting everyday surroundings, but development of more effective disinfectants imperative. A series studies focusing on cationic antimicrobials resulted the a safe disinfectant, DEA-171, which provides ≥99.98% inhibition all variants within 1 min.
The novel sudden acute respiratory syndrome coronavirus 2 is an enveloped virus currently causing severe illness and death worldwide. Common antiseptics such as alcohol have some efficacy in disinfecting everyday surroundings, but development of more effective disinfectants imperative. A series studies focusing on cationic antimicrobials resulted the a safe disinfectant, DEA-171, which provides ≥99.98% inhibition all variants within 1 min.
The novel sudden acute respiratory syndrome coronavirus 2 is an enveloped virus currently causing severe illness and death worldwide. Common antiseptics such as alcohol have some efficacy in disinfecting everyday surroundings, but development of more effective disinfectants imperative. A series studies focusing on cationic antimicrobials resulted the a safe disinfectant, DEA-171, which provides ≥99.98% inhibition all variants within 1 min.