A5015074593- Spine and Intervertebral Disc Pathology
- Spinal Fractures and Fixation Techniques
- Cervical and Thoracic Myelopathy
- Scoliosis diagnosis and treatment
- Spinal Cord Injury Research
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Spinal Hematomas and Complications
- Musculoskeletal pain and rehabilitation
- Spinal Dysraphism and Malformations
- Pelvic and Acetabular Injuries
- Nerve Injury and Rehabilitation
- Neurofibromatosis and Schwannoma Cases
- Medical Imaging and Analysis
- Management of metastatic bone disease
- Spondyloarthritis Studies and Treatments
- Vascular Malformations Diagnosis and Treatment
- Tissue Engineering and Regenerative Medicine
- Neurogenetic and Muscular Disorders Research
- Stroke Rehabilitation and Recovery
- Cerebral Palsy and Movement Disorders
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
Keio University
2016-2025
University Health Network
2015-2025
Institute for Conflict Research
2024
Keio University Hospital
2012-2024
Niigata University
2023
Kitasato University
2023
Research Organization of Information and Systems
2018-2023
Toronto Western Hospital
2015-2021
Kudanzaka Hospital
2021
Chiba University
2021
Mesenchymal stem cells (MSCs) are defined as that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue culture, the equivalent not identified vivo little is known about their physiological roles or even exact location. In this study, we used phenotypic, morphological, functional criteria identify prospectively isolate a subset of (PDGFRα+Sca-1+CD45−TER119−) adult mouse bone marrow. Individual generated colonies...
Various types of induced pluripotent stem (iPS) cells have been established by different methods, and each type exhibits biological properties. Before iPS cell-based clinical applications can be initiated, detailed evaluations the cells, including their differentiation potentials tumorigenic activities in contexts, should investigated to establish safety effectiveness for cell transplantation therapies. Here we show directed neural murine examine therapeutic potential a mouse spinal cord...
Guideline development.The objective of this study is to develop guidelines that outline how best manage (1) patients with mild, moderate, and severe myelopathy (2) nonmyelopathic evidence cord compression or without clinical symptoms radiculopathy.Five systematic reviews the literature were conducted synthesize on disease natural history; risk factors progression; efficacy, effectiveness, safety nonoperative surgical management; impact preoperative duration severity treatment outcomes;...
In chronic kidney disease, fibroblast dysfunction causes renal fibrosis and anemia. Renal is mediated by the accumulation of myofibroblasts, whereas anemia reduced production fibroblast-derived erythropoietin, a hormone that stimulates erythropoiesis. Despite their importance in origin regulatory mechanism fibroblasts remain unclear. Here, we have demonstrated majority erythropoietin-producing healthy originate from myelin protein zero–Cre (P0-Cre) lineage-labeled extrarenal cells, which...
To develop recommendations on the timing of surgical decompression in patients with traumatic spinal cord injury (SCI) and central syndrome.A systematic review literature was conducted to address key relevant questions. A multidisciplinary guideline development group used this information, along their clinical expertise, for SCI syndrome. Based GRADE, a strong recommendation is worded as "we recommend," whereas weak presented suggest."Conclusions from included (1) isolated studies reported...
The objective of this guideline is to outline the appropriate use methylprednisolone sodium succinate (MPSS) in patients with acute spinal cord injury (SCI).A systematic review literature was conducted address key questions related MPSS SCI. A multidisciplinary Guideline Development Group used information, combination their clinical expertise, develop recommendations for MPSS. Based on GRADE (Grading Recommendation, Assessment, and Evaluation), a strong recommendation worded as "we...
Our group has conducted extensive basic and preclinical studies of the use human induced pluripotent cell (iPSC)-derived neural stem/progenitor (hiPSC-NS/PC) grafts in models spinal cord injury (SCI). Evidence from animal experiments suggests this approach is safe effective. We are preparing to initiate a first-in-human clinical study hiPSC-NS/PC transplantation subacute SCI.NS/PCs were prepared at Good Manufacturing Practice-grade processing facility Osaka National Hospital using...
Study Design Clinical practice guideline development. Objectives Acute spinal cord injury (SCI) can result in devastating motor, sensory, and autonomic impairment; loss of independence; reduced quality life. Preclinical evidence suggests that early decompression the may help to limit secondary injury, reduce damage neural tissue, improve functional outcomes. Emerging indicates “early” surgical completed within 24 hours also improves neurological recovery patients with acute SCI. The...
We report the presence of neural crest-derived corneal precursors (COPs) that initiate spheres by clonal expansion from a single cell. COPs expressed stem cell markers nestin, Notch1, Musashi-1, and ABCG2 showed side population phenotype. were multipotent with ability to differentiate into adipocytes, chondrocytes, as well cells, shown expression beta-III-tubulin, glial fibrillary acidic protein, neurofilament-M. COP prepared E/nestin-enhanced green fluorescent protein (EGFP) mice induction...
Riluzole is a sodium channel-blocking agent used in treating amyotrophic lateral sclerosis. It has been approved by the U.S. Food and Drug Administration, Canadian Australian authorities, many other countries. A phase I trial of riluzole for acute spinal cord injury (SCI) provided safety pharmacokinetic data suggested neuroprotective benefits. IIB/III double-blinded randomized controlled (RCT) started January 2014 ( https://clinicaltrials.gov , NCT01597518). This article describes...
Highlights•The iCaspase9 system induced apoptosis of iPSCs and all iPSC derivatives in vitro•Grafted iPSC-NS/PCs formed teratomas non-teratomatous neural tumors•The prevented tumor progression adverse effects with tumor•CID was demonstrated to be an effective inducer even the CNS protected by BBBSummaryHuman pluripotent stem cells (iPSCs) are promising regenerative medicine. However, risks teratoma formation overgrowth transplanted continue major hurdles that must overcome. Here, we examined...
Study Design. A retrospective review of 281 consecutive cases adult spine deformity (ASD) surgery (age 55 ± 19 yrs, 91% female, follow-up 4.3 1.9 yrs) from a multicenter database. Objective. To compare the value and predictive ability 5-item modified frailty index (mFI-5) to conventional 11-item (mFI-11) for severe adverse events (SAEs). Summary Background Data. Several recent studies have described associations between surgical complications. However, power usefulness mFI-5 not been proven....
Abstract Cell transplantation therapy utilizing neural precursor cells (NPCs) is a conceptually attractive strategy for traumatic spinal cord injury (SCI) to replace lost cells, remyelinate denuded host axons and promote tissue sparing. However, the number of mature oligodendrocytes that differentiate from typical NPCs remains limited. Herein, we describe novel approach bias differentiation directly reprogrammed human (drNPCs) toward more oligodendrogenic fate (oNPCs) while preserving their...
Traumatic spinal cord injuries (SCIs) affect millions of people worldwide; the majority whom are in chronic phase their injury. Unfortunately, most current treatments target acute/subacute injury as microenvironment chronically injured consists a well-established glial scar with inhibitory chondroitin sulfate proteoglycans (CSPGs) which acts potent barrier to regeneration. It has been shown that CSPGs can be degraded vivo by intrathecal Chondroitinase ABC (ChABC) produce more permissive...
Study Design. A propensity-matched comparison of risk factors for proximal junctional failure (PJF), which is a symptomatic kyphosis developing after corrective surgery adult spinal deformity (ASD). Objective. To elucidate the role bone strength PJF. Summary Background Data. PJF, devastating complication ASD, often recurs even revision surgery. Most studies PJF are retrospective and have selection bias in surgical strategy, making it difficult to identify modifiable factors. Methods. We...
Neural progenitor cell (NPC) transplantation is a promising strategy for the treatment of spinal cord injury (SCI). In this study, we show that injury-induced Notch activation in microenvironment biases fate transplanted NPCs toward astrocytes rodents. screen potential clinically relevant factors to modulate signaling, identified glial cell-derived neurotrophic factor (GDNF). GDNF attenuates signaling by mediating delta-like 1 homolog (DLK1) expression, which independent GDNF's effect on...
While rapid advancements in regenerative medicine strategies for spinal cord injury (SCI) have been made, most research this field has focused on the early stages of incomplete injury. However, majority patients experience chronic severe injury; therefore, treatments these situations are fundamentally important. Here, we hypothesized that environmental modulation via a clinically relevant hepatocyte growth factor (HGF)-releasing scaffold and human iPS cell-derived neural stem/progenitor...
Abstract Chitosan has various tissue regeneration effects. This study was designed to investigate the nerve effect of Schwann cell (SC)-encapsulated chitosan-collagen hydrogel conduit (CCN) transplanted into a rat model sciatic defect. We prepared CCN consisting an outer layer chitosan and inner collagen encapsulate intended cells. Rats with 10-mm defect were treated SCs encapsulated in (CCN+), without (CCN−), SC-encapsulated silicone tube (silicone+), autologous transplanting (auto)....
Spinal cord injury (SCI) often results in various long-term sequelae, and chronically injured spinal cords exhibit a refractory feature, showing limited response to cell transplantation therapies. To our knowledge, no preclinical studies have reported treatment approach with surpassing those of comprising rehabilitation alone. In this study rats SCI, we propose novel combined therapy involving semaphorin 3A inhibitor (Sema3Ai), which enhances axonal regeneration, as the third element...
Transplantation of neural stem/progenitor cells (NS/PCs) following the sub-acute phase spinal cord injury (SCI) has been shown to promote functional recovery in rodent models. However, types most effective for treating SCI have not clarified. Taking advantage our recently established neurosphere-based culture system ES cell-derived NS/PCs, which primary neurospheres (PNS) and passaged secondary (SNS) exhibit neurogenic gliogenic potentials, respectively, here we examined distinct effects...