A5068425017- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Biomedical Ethics and Regulation
- Neurogenesis and neuroplasticity mechanisms
- Spinal Cord Injury Research
- Nerve injury and regeneration
- Stroke Rehabilitation and Recovery
- Cerebral Palsy and Movement Disorders
- Musculoskeletal pain and rehabilitation
- Scoliosis diagnosis and treatment
- Health and Wellbeing Research
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Complementary and Alternative Medicine Studies
- Health and Well-being Studies
- Occupational Health and Performance
- Knee injuries and reconstruction techniques
- Nerve Injury and Rehabilitation
- Cytokine Signaling Pathways and Interactions
- RNA regulation and disease
- Neuroethics, Human Enhancement, Biomedical Innovations
- Musculoskeletal Disorders and Rehabilitation
- Healthcare Education and Workforce Issues
- Health Systems, Economic Evaluations, Quality of Life
Keio University
2015-2025
Keio University Hospital
2015-2020
StemCells (United States)
2018
Sapporo Science Center
2017
Our group has conducted extensive basic and preclinical studies of the use human induced pluripotent cell (iPSC)-derived neural stem/progenitor (hiPSC-NS/PC) grafts in models spinal cord injury (SCI). Evidence from animal experiments suggests this approach is safe effective. We are preparing to initiate a first-in-human clinical study hiPSC-NS/PC transplantation subacute SCI.NS/PCs were prepared at Good Manufacturing Practice-grade processing facility Osaka National Hospital using...
Murine- and human-induced pluripotent stem cell-derived neural stem/progenitor cells (iPSC-NS/PCs) promote functional recovery following transplantation into the injured spinal cord in rodents primates. Although remyelination of spared demyelinated axons is a critical mechanism regeneration cord, human iPSC-NS/PCs predominantly differentiate neurons both vitro vivo. We therefore took advantage our recently developed protocol to obtain oligodendrocyte precursor cell-enriched report benefits...
Highlights•The iCaspase9 system induced apoptosis of iPSCs and all iPSC derivatives in vitro•Grafted iPSC-NS/PCs formed teratomas non-teratomatous neural tumors•The prevented tumor progression adverse effects with tumor•CID was demonstrated to be an effective inducer even the CNS protected by BBBSummaryHuman pluripotent stem cells (iPSCs) are promising regenerative medicine. However, risks teratoma formation overgrowth transplanted continue major hurdles that must overcome. Here, we examined...
Abstract Most studies targeting chronic spinal cord injury (SCI) have concluded that neural stem/progenitor cell (NS/PC) transplantation exerts only a subclinical recovery; this in contrast to its remarkable effect on acute and subacute SCI. To determine whether the addition of rehabilitative intervention enhances NS/PC for SCI, we used thoracic SCI mouse models compare manifestations secondary both treadmill training two therapies combined, with control group. Significant locomotor recovery...
Neural stem/progenitor cells (NS/PCs) derived from human induced pluripotent stem (hiPSCs) are considered to be a promising cell source for cell-based interventions that target CNS disorders. We previously reported transplanting certain hiPSC-NS/PCs in the spinal cord results tumor-like overgrowth of and subsequent deterioration motor function. Remnant immature should removed or into more mature types avoid adverse effects hiPSC-NS/PC transplantation. Because Notch signaling plays role...
Understanding how the transcription factor signal transducer and activator of transcription–3 (STAT3) controls glial scar formation may have important clinical implications. We show that astrocytic STAT3 is associated with greater amounts secreted MMP2, a crucial protease in formation. Moreover, we report inhibits small GTPase RhoA thereby actomyosin tonus, adhesion turnover, migration reactive astrocytes, as well corralling leukocytes vitro. The inhibition by involves ezrin, phosphorylation...
Abstract Tumorigenesis is an important problem that needs to be addressed in the field of human stem/progenitor cell transplantation for treatment subacute spinal cord injury (SCI). When certain “tumorigenic” lines are transplanted into SCI mice model, there initial improvement motor function, followed by abrupt deterioration secondary effect tumor growth. A significant proportion cells remains undifferentiated after and thought increase risk tumorigenesis. In this study, using lentiviral...
The risk of tumorigenicity is a hurdle for regenerative medicine using induced pluripotent stem cells (iPSCs). Although teratoma formation readily distinguishable, the malignant transformation iPSC derivatives has not been clearly defined due to insufficient analysis histology and phenotype. In present study, we evaluated neural stem/progenitor (NSPCs) generated from integration-free human peripheral blood mononuclear cell (PBMC)-derived iPSCs (iPSC-NSPCs) following transplantation into...
Previous studies have demonstrated that transplantation of neural stem/progenitor cells (NS/PCs) into the lesioned spinal cord can promote functional recovery following incomplete injury (SCI) in animal models. However, this strategy is insufficient complete SCI because gap at lesion epicenter. To obtain a mouse model SCI, study uses novel collagen-based microfiber as scaffold for engrafted NS/PCs. We hypothesized NS/PC-microfiber combination would facilitate closure well transplant survival...
Stem cells represent a potential cellular resource in the development of regenerative medicine approaches to treatment pathologies which specific are degenerated or damaged by genetic abnormality, disease, injury. Securing sufficient supplies suited demands cell transplantation, however, remains challenging, and establishment safe efficient banking procedures is an important goal. Cryopreservation allows storage stem for prolonged time periods while maintaining them adequate condition use...
To achieve the goal of a first-in-human trial for human induced pluripotent stem cell (hiPSC)-based transplantation treatment various diseases, allogeneic leukocyte antigen (HLA)-matched hiPSC banks represent realistic tool from perspective quality control and cost performance. Furthermore, considering limited therapeutic time-window acute injuries, including neurotraumatic an iPS bank is potential interest. However, due to relatively immunoprivileged environment central nervous system, it...
Objectives To examine the association between knee pain and function depressive symptoms in older Japanese adults. Design Community‐based prospective cohort study. Setting Kurabuchi Town, Gumma Prefecture, Japan. Participants Individuals aged 65 (N = 573; n 260 men, 313 women) without participated baseline examinations 2005 2006; 95.6% follow‐up interviews (2007–08). Measurements Degree of functional impairment was assessed at using a self‐administered questionnaire based on an English...
Resolving the immunogenicity of cells derived from induced pluripotent stem (iPSCs) remains an important challenge for cell transplant strategies that use banked allogeneic cells. Thus, we evaluated mouse fetal neural stem/progenitor (fetus-NSPCs) and iPSC-derived (iPSC-NSPCs) both in vitro vivo. Flow cytometry revealed low expression immunological surface antigens, these survived all mice when transplanted syngeneically into subcutaneous tissue spinal cord. In contrast, transplantation was...
Body weight-supported treadmill training with the voluntary driven exoskeleton (VDE-BWSTT) has been shown to improve gait function of patients chronic spinal cord injury. However, little is known whether VDE-BWSTT can effectively trunk In this open-label, single-arm study, nine injury at cervical or thoracic level (six males and three females, aged 37.8 ± 15.6 years, time since 51.1 31.8 months) who underwent outpatient program Keio University Hospital, Japan from September 2017 March 2019...
Previous epidemiological surveys conducted in Japan highlighted problems with conventional approaches to treating chronic musculoskeletal pain. On the basis of prior studies, we initiated "longitudinal investigation pain" 2010. In our first two reports, revealed a high prevalence pain, low satisfaction treatment, and reduced quality life. Those severe consistent back pain had highest risk persisting The factors for developing also included working professional, managerial, or...
The progression of hyperkyphosis is a significant factor in declining general health. purpose this study was to investigate whether muscle strength and/or mobility associated with the elderly people.As part cohort community-dwelling people, handgrip and (evaluated Timed Up Go Test: TUG) were assessed at baseline (2005-2006). Kyphosis also evaluated follow-up examinations 4 years later, block method. To exclude measurement error, kyphosis defined as increase 2 or more blocks. association each...
This cohort study conducted in Japan showed that severe age-related kyphosis was visually detected. The visual assessment of associated with declines ADL, suggesting we can easily identify people at high risk to develop future ADL reduction the community setting. Age-related is related activities daily living (ADL). Its conventional diagnosis has been made by orthopedic surgeons and trained examiners using specialized equipment such as X-rays. We investigated whether classification...
Spinal cord injury (SCI) is a devastating that causes permanent neurological dysfunction. To develop new treatment strategy for SCI, clinical trial of transplantation human-induced pluripotent stem cell-derived neural precursor cells (NPCs) in patients the subacute phase SCI was recently initiated. The formation synaptic connections with host tissues one therapeutic mechanisms cell transplantation, and this beneficial efficacy has been directly demonstrated using chemogenetic tool. This...
The aim of this study was to investigate whether body weight-supported treadmill training with voluntary-driven exoskeleton (VDE-BWSTT) improves the quality life (QOL) persons chronic spinal cord injury (SCI). Nineteen individuals SCI walking limitation underwent a total 20 sessions VDE-BWSTT using hybrid assistant limb. QOL measured Short Form-36v2 (SF-36v2) questionnaire at preintervention and postintervention. Walking Index for SCI-II (WISCI-II), Functional Independence Measure (FIM)...
The effect of knee osteoarthritis, which causes pain, on higher-level functional competence (HLFC) is not clear.To clarify the pain HLFC in older people.Community-based prospective cohort study.Kurabuchi town, Gumma prefecture, Japan.Community-dwelling individuals aged 65 and older.A total 808 residents participated to baseline examinations. frequency degree impairment resulting from were asked at (2005-2006) via a self-administered questionnaire Japanese based an English version Western...