A5089490587- Spinal Cord Injury Research
- Cervical and Thoracic Myelopathy
- Spinal Dysraphism and Malformations
- Nerve Injury and Rehabilitation
- Neuroscience of respiration and sleep
- Nerve injury and regeneration
- Spine and Intervertebral Disc Pathology
- Aortic Disease and Treatment Approaches
- Spinal Hematomas and Complications
- Spinal Fractures and Fixation Techniques
- Antimicrobial Peptides and Activities
- Neurogenetic and Muscular Disorders Research
- Stroke Rehabilitation and Recovery
- Cytokine Signaling Pathways and Interactions
- Neuropeptides and Animal Physiology
- Neurogenesis and neuroplasticity mechanisms
- Adenosine and Purinergic Signaling
- Nitric Oxide and Endothelin Effects
- Neural dynamics and brain function
- Supramolecular Self-Assembly in Materials
- Congenital Diaphragmatic Hernia Studies
- Diabetic Foot Ulcer Assessment and Management
- Fatty Acid Research and Health
- Family and Disability Support Research
- Cardiac Ischemia and Reperfusion
Medical College of Wisconsin
2020-2025
Milwaukee VA Medical Center
2020-2023
University Health Network
2011-2021
Krembil Research Institute
2016-2021
Krembil Foundation
2016-2017
University of Toronto
2013-2014
Toronto Western Hospital
2012-2013
Western Research Institute
2012
Wayne State University
2008-2009
Traumatic spinal cord injuries (SCIs) affect millions of people worldwide; the majority whom are in chronic phase their injury. Unfortunately, most current treatments target acute/subacute injury as microenvironment chronically injured consists a well-established glial scar with inhibitory chondroitin sulfate proteoglycans (CSPGs) which acts potent barrier to regeneration. It has been shown that CSPGs can be degraded vivo by intrathecal Chondroitinase ABC (ChABC) produce more permissive...
The pathophysiology of spinal cord injury (SCI) involves post-traumatic inflammation and glial scarring which interfere with repair recovery. Self-assembling peptides (SAPs) are molecules designed for tissue engineering. Here, we tested the performance K2(QL)6K2 (QL6), a SAP that attenuates scarring, facilitates functional We injected QL6 into rats 24 h after clip compression SCI. led to significant reduction in apoptosis, astrogliosis. It also resulted preservation as determined by...
Riluzole, a sodium/glutamate antagonist has shown promise as neuroprotective agent. It is licensed for amyotrophic lateral sclerosis and in clinical trial development spinal cord injury (SCI). This study investigated the therapeutic time-window pharmacokinetics of riluzole rodent model cervical SCI. Rats were treated with (8 mg/kg) at 1 hour (P1) 3 hours (P3) after or vehicle. Afterward, P1 P3 groups received (6 (mg/kg) every 12 7 days. Both animals had significant improvements locomotor...
Abstract Background Evidence suggests that the inflammatory events in acute phase of spinal cord injury (SCI) exacerbate initial trauma to leading poor functional recovery. As a result, minimizing detrimental aspects response after SCI is promising treatment strategy. In this regard, immunoglobulin G (IgG) from pooled human serum candidate. Due its putative, though poorly characterized immuno-modulatory effects, IgG has been used clinically treat neuroinflammatory disorders such as...
Abstract Spinal cord injury has a significant societal and personal impact. Although the majority of injuries involve cervical spinal cord, few studies cell transplantation have used clinically relevant models injury, limiting translation into clinical trials. Given this knowledge gap, we sought to examine effects neural stem/precursor (NPC) transplants in rodent model bilateral contusion-compression injury. Bilateral C6-level clip were performed rats, which then blindly randomized at 2...
Cervical spinal cord injury (SCI) can result in devastating functional deficits that involve the respiratory and hand function. The mammalian has limited ability to regenerate restore meaningful recovery following SCI. Riluzole, 2-amino-6-trifluoromethoxybenzothiazole, an anti-glutamatergic drug been shown reduce excitotoxicity confer neuroprotection at site of experimental Based on promising preclinical studies, riluzole is currently under Phase III clinical trial for treatment SCI...
Despite the increasing incidence and prevalence of cervical spinal cord injury (cSCI), we lack clinically relevant animal models that can be used to study pathomechanisms this test new therapies. Here, characterize a moderate contusion-compression model in rats is similar incomplete traumatic cSCI humans. We characterized effects 18-g clip-compression at level C6 over an 8-week recovery period. Using Luxol fast blue/hematoxylin-eosin staining combination with quantitative stereology,...
Abstract Degenerative cervical myelopathy is a common condition resulting from chronic compression of the spinal cord by degenerating structures spine. present wide range outcomes, and biological factors underlying this variability are poorly understood. Previous studies have found elevated MIR21-5p in sub-acute neuroinflammatory environment after injury. As neuroinflammation major feature degenerative myelopathy, we hypothesized that may be particularly relevant to disease pathobiology,...
Cervical spondylotic myelopathy (CSM) is the most common cause of spinal cord impairment worldwide and a risk factor for traumatic central syndrome. Despite advances in surgery, there are no effective neuroprotective treatments CSM, which reflects limited understanding its pathophysiology. In order to develop therapeutic strategies, we have developed novel rat model chronic progressive cervical compression that mimics CSM. A titanium-screw-based device (CCD) was designed achieve at C6 level....
Traumatic injury to the lumbar spinal cord results in complex central and peripheral nervous tissue damage causing significant neurobehavioral deficits personal/social adversity. Although injuries are common humans, there few clinically relevant models of (SCI). This article describes a novel SCI model rat. The effects moderate (20 g), moderate-to-severe (26 g) severe (35 g, 56 clip impact-compression at level L1-L2 (vertebral T11-T12) were assessed using several neurobehavioral,...
Following spinal cord injury (SCI) there are drastic changes that occur in the microvasculature, including ischemia, hemorrhage, endothelial cell death and blood-spinal barrier disruption. Vascular growth factor-A (VEGF-A) is a pleiotropic factor recognized for its pro-angiogenic properties; however, VEGF has recently been shown to provide neuroprotection. We hypothesized delivery of AdV-ZFP-VEGF – an adenovirally delivered bio-engineered zinc-finger transcription promotes endogenous VEGF-A...
Abstract Background Spinal cord injury (SCI) is a devastating condition with substantial functional and social morbidity. Previous research has established that the neuroinflammatory response plays significant role in damage post-SCI. However, global immunosuppressive therapies have demonstrated mixed results. As result, more specific modulating inflammation after are needed. In this regard, into cytokine signaling cytokines of gp130 family including IL-6 leukemia inhibitory factor (LIF)...
Background/Objective: High cervical spinal cord hemisection interrupts descending respiratory drive from the rostral ventral group in medulla to ipsilateral phrenic motoneurons. Hemisection results paralysis of hemidiaphragm. Chronic administration rolipram, a specific phosphodiesterase-IV inhibitor, promotes synaptic plasticity and restores nerve function after high lesion. Here, we test hypothesis that an acute rolipram will increase medullary levels 3′,5′-cyclic adenosine monophosphate...