A5069453818- Pulmonary Hypertension Research and Treatments
- Pluripotent Stem Cells Research
- Cardiovascular Function and Risk Factors
- Tissue Engineering and Regenerative Medicine
- Cardiac Valve Diseases and Treatments
- Atrial Fibrillation Management and Outcomes
- Cardiac Arrhythmias and Treatments
- Cardiac electrophysiology and arrhythmias
- Heart Failure Treatment and Management
- Congenital heart defects research
- Acute Myocardial Infarction Research
- Cardiac Imaging and Diagnostics
- Cardiac Fibrosis and Remodeling
- CRISPR and Genetic Engineering
- Coronary Interventions and Diagnostics
- Cardiac pacing and defibrillation studies
- Infective Endocarditis Diagnosis and Management
- Congenital Heart Disease Studies
- Cardiomyopathy and Myosin Studies
- Ion channel regulation and function
- Antiplatelet Therapy and Cardiovascular Diseases
- Venous Thromboembolism Diagnosis and Management
- Mesenchymal stem cell research
- Adipose Tissue and Metabolism
- Cardiac Ischemia and Reperfusion
Keio University Hospital
2016-2025
Keio University
2015-2024
Fukuda Denshi (Japan)
2015-2024
Japan Pediatric Society
2023-2024
UCLA Health
2023
ORCID
2021
Shimizu (Japan)
2006-2021
Edwards Lifesciences (Switzerland)
2021
Creative Commons
2021
Mount Sinai Beth Israel
2020
We have isolated a cardiomyogenic cell line (CMG) from murine bone marrow stromal cells. Stromal cells were immortalized, treated with 5-azacytidine, and spontaneously beating repeatedly screened. The showed fibroblast-like morphology, but the morphology changed after 5-azacytidine treatment in ∼30% of cells; they connected adjoining one week, formed myotube-like structures, began two weeks, beat synchronously three weeks. They expressed atrial natriuretic peptide brain stained anti-myosin,...
Acute myocardial infarction (AMI) is a common and lethal heart disease, the recruitment of fibroblastic cells to infarct region essential for cardiac healing process. Although stiffness extracellular matrix in myocardium associated with healing, molecular mechanism not fully understood. We show that periostin, which matricellular protein, important process after AMI. The expression periostin protein was abundant border human mouse hearts generated periostin−/− mice found no morphologically...
Background— Chronic thromboembolic pulmonary hypertension leads to and right-sided heart failure. The purpose of this study was investigate the efficacy percutaneous transluminal angioplasty (PTPA) for treatment chronic hypertension. Methods Results— Twenty-nine patients with underwent PTPA. One patient had a wiring perforation as complication PTPA died 2 days after procedure. In remaining 28 patients, did not produce immediate hemodynamic improvement at time However, follow-up (6.0 ± 6.9...
Heart disease remains a leading cause of death worldwide. Owing to the limited regenerative capacity heart tissue, cardiac therapy has emerged as an attractive approach. Direct reprogramming human fibroblasts (HCFs) into cardiomyocytes may hold great potential for this purpose. We reported previously that induced cardiomyocyte-like cells (iCMs) can be directly generated from mouse in vitro and vivo by transduction three transcription factors: Gata4, Mef2c, Tbx5, collectively termed GMT. In...
Rationale : The (pro)renin receptor [(P)RR], encoded in ATP6AP2 , plays a key role the activation of local renin-angiotensin system (RAS). A truncated form (P)RR, termed M8.9, was also found to be associated with vacuolar H + -ATPase (V-ATPase), implicating non–RAS-related function ATP6AP2. Objective We investigated (P)RR/ATP6AP2 murine cardiomyocytes. Methods and Results Cardiomyocyte-specific ablation Atp6ap2 resulted lethal heart failure; cardiomyocytes contained RAB7-...
Background— Inflammation and immune responses are integral components in the healing process after myocardial infarction. We previously reported dendritic cell (DC) infiltration infarcted heart; however, precise contribution of DC postinfarction is unclear. Methods Results— Bone marrow cells from CD11c-diphtheria toxin receptor/green fluorescent protein transgenic mice were transplanted into lethally irradiated wild-type recipient mice. After reconstitution bone marrow–derived cells, treated...
After myocardial infarction (MI), massive cell death in the myocardium initiates fibrosis and scar formation, leading to heart failure. We recently found that a combination of 3 cardiac transcription factors, Gata4, Mef2c, Tbx5 (GMT), reprograms fibroblasts directly into functional cardiomyocytes vitro.To investigate whether viral gene transfer GMT infarcted hearts induces cardiomyocyte generation.Coronary artery ligation was used generate MI mouse. In vitro transduction retrovirus converted...
The semiquantitative Clinical Frailty Scale (CFS) is a simple tool to assess patients' frailty and has been shown correlate with mortality in elderly patients even when evaluated by nongeriatricians. aim of the current study was determine prognostic value CFS who underwent transcatheter aortic valve replacement.We utilized OCEAN (Optimized Catheter Valvular Intervention) Japanese multicenter registry review data 1215 replacement. Patients were categorized into 5 groups based on stages: 1-3,...
Chronic inflammation in visceral adipose tissue (VAT) precipitates the development of cardiometabolic disorders. Although changes T cell function associated with obesity are thought to affect chronic VAT inflammation, specific features these remain elusive. Here, we have determined that a high-fat diet (HFD) caused preferential increase and accumulation CD44hiCD62LloCD4+ cells constitutively express PD-1 CD153 B cell-dependent manner VAT. These possessed characteristics cellular senescence...
Both osteopontin (OPN) and galectin-3 have been implicated in phagocytic clearance of dead cells reparative fibrosis during wound healing. CD206+ macrophages are involved tissue repair through phagocytosis after myocardial infarction (MI). However, the relationship among OPN, galectin-3, macrophage polarization context MI remains unclear.The time course Spp1 (encoding OPN) expression heart showed a strong activation on day 3 MI. To identify where body which transcriptional activity increased...
Rationale: In-hospital outcomes are generally acceptable in patients with type B dissection; however, some present undesirable complications, such as aortic expansion and rupture. Excessive inflammation is an independent predictor of adverse clinical outcomes. Objective: We have investigated the underlying mechanisms catastrophic complications after acute dissection (AAD) mice. Methods Results: When angiotensin II was administered lysyl oxidase inhibitor–preconditioned mice, AAD emerged...