A5055863647- Ruminant Nutrition and Digestive Physiology
- Renal Transplantation Outcomes and Treatments
- Effects of Environmental Stressors on Livestock
- Organ Transplantation Techniques and Outcomes
- Cardiac Valve Diseases and Treatments
- Renal Diseases and Glomerulopathies
- Agriculture Sustainability and Environmental Impact
- Cardiovascular Function and Risk Factors
- Genetic and phenotypic traits in livestock
- Organ Donation and Transplantation
- Pluripotent Stem Cells Research
- Fibroblast Growth Factor Research
- Neurological Complications and Syndromes
- Reproductive Physiology in Livestock
- Trace Elements in Health
- Animal Nutrition and Physiology
- Kruppel-like factors research
- Cancer, Hypoxia, and Metabolism
- Cardiac electrophysiology and arrhythmias
- Congenital Heart Disease Studies
- Neuroscience and Neural Engineering
- Cardiac pacing and defibrillation studies
- Liver Disease and Transplantation
- Rice Cultivation and Yield Improvement
- CRISPR and Genetic Engineering
Institute of Livestock and Grassland Science
2018-2024
Shimane Prefectural Institute of Public Health and Environmental Sciences
2015-2024
Kanetsu Hospital
2023
National Agriculture and Food Research Organization
2008-2023
Japan International Research Center for Agricultural Sciences
2008-2022
Tay Bac University
2022
Central Region Agricultural Research Center
2020
Tohoku University
2019
Fujita Animal Hospital
2019
Kyushu Okinawa Agricultural Research Center
2006-2018
Zinc is an essential trace element required for enzymatic activity and maintaining the conformation of many transcription factors; thus, zinc homeostasis tightly regulated. Although affects several signaling molecules may act as a neurotransmitter, it remains unknown whether acts intracellular second messenger capable transducing extracellular stimuli into events. In this study, we report that cross-linking high affinity immunoglobin E receptor (Fcε I [FcεRI]) induced release free from...
Heart disease remains a leading cause of death worldwide. Owing to the limited regenerative capacity heart tissue, cardiac therapy has emerged as an attractive approach. Direct reprogramming human fibroblasts (HCFs) into cardiomyocytes may hold great potential for this purpose. We reported previously that induced cardiomyocyte-like cells (iCMs) can be directly generated from mouse in vitro and vivo by transduction three transcription factors: Gata4, Mef2c, Tbx5, collectively termed GMT. In...
Long QT syndrome (LQTS) is an inheritable and life-threatening disease; however, it often difficult to determine disease characteristics in sporadic cases with novel mutations, more precise analysis necessary for the successful development of evidence-based clinical therapies. This study thus sought better characterize ion channel cardiac disorders using induced pluripotent stem cells (iPSCs). We reprogrammed somatic from a patient LQTS controls, differentiated them into cardiomyocytes...
Zinc is an essential component for the catalytic activity of numerous zinc-requiring enzymes. However, until recently little has been known about molecules involved in pathways required supplying zinc to these We showed (Suzuki, T., Ishihara, K., Migaki, H., Matsuura, W., Kohda, A., Okumura, Nagao, M., Yamaguchi-Iwai, Y., and Kambe, T. (2005) J. Biol. Chem. 280, 637-643) that transporters, ZnT5 ZnT7, are activation enzymes, alkaline phosphatases (ALPs), by transporting into lumens Golgi...
Numerous proteins are properly folded by binding with zinc during their itinerary in the biosynthetic-secretory pathway. Several transporters have been implicated entry into secretory compartments from cytosol, but precise roles poorly understood. We report here that two (ZnT5 and ZnT7) localized apparatus responsible for loading to alkaline phosphatases (ALPs) glycosylphosphatidylinositol-anchored membrane exposed extracellular site. Disruption of ZnT5 gene DT40 cells decreased ALP activity...
The majority of CDF/ZnT zinc transporters form homo-oligomers. However, ZnT5, ZnT6, and their orthologues hetero-oligomers in the early secretory pathway where they load onto zinc-requiring enzymes maintain functions. details this hetero-oligomerization remain to be elucidated, much more is known about homo-oligomerization that occurs other family proteins. Here, we addressed issue using co-immunoprecipitation experiments, mutagenesis, chimera studies hZnT5 hZnT6 chicken DT40 cells deficient...
Abstract Cardiac regenerative therapy with human pluripotent stem cells (hPSCs), such as embryonic and induced cells, has been hampered by the lack of efficient strategies for expanding functional cardiomyocytes (CMs) to clinically relevant numbers. The development massive suspension culture system (MSCS) shed light on this critical issue, although it remains unclear how hPSCs could differentiate into CMs using a MSCS. proliferative rate differentiating in MSCS was equivalent that cultures...
Mitochondrial diseases are heterogeneous disorders, caused by mitochondrial dysfunction. Mitochondria not regulated solely nuclear genomic DNA but DNA. It is difficult to develop effective therapies for disease because of the lack models. myopathy, encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) one major diseases. The aim this study was generate MELAS-specific induced pluripotent stem cells (iPSCs) demonstrate that MELAS-iPSCs can be models disease. We successfully...
Because of lack deceased donors in Japan, there has been a need to expand the eligibility criteria for live kidney donation. To assess indications donation glucose intolerance (GI), we analyzed perioperative complications associated with donor nephrectomies performed at our institution and followed up long-term consequences.The 444 were divided into two groups based on results 75-g oral tolerance test: GI group (n=71) who showed diabetic (n=27) or impaired (n=44) pattern, non-GI (n=373)...
This study was conducted to assess the effects of replacing rice straw with different proportions cassava pulp on growth performance, feed intake, digestibility, rumen microbial population, energy partitioning and efficiency metabolizable utilization in beef cattle.Eighteen yearling Thai native cattle (Bos indicus) an average initial body weight (BW) 98.3±12.8 kg were allocated one three dietary treatments fed ad libitum for 149 days a randomized complete block design. Three using (100, 300,...
A new animal calorimeter using a ventilated hood was constructed in Thailand. The recovery rates of our head cage were 96.5%, 97.0%, 95.7%, and 101.8%. (95.7-101.8%) are reliable compared with Japanese whole-body chambers. 99% CO2 time an average 14 min. It shorter than chambers because smaller effective volume higher flow rate. Our system excels at dynamic measurement heat production. We started accumulating data concerning the energy value Pangola grass (Digitaria eriantha) 4 Brahman...
We investigated the changes in plasma concentrations of oxidative stress markers periparturient dairy cows hot conditions. This study was performed on 10 from day -5 to after parturition. The experimental duration divided into three periods: period 1 (5 days before parturition); 2 and 3 (from 6 parturition). average rectal temperature during more than 39 °C. Plasma aspartate aminotransferase activities periods were higher that (P<0.05). Blood urea nitrogen concentration lower those total...
Proliferation of pancreatic β-cells is an important mechanism underlying β-cell mass adaptation to metabolic demands. Increasing by regeneration may ameliorate or correct both type 1 and 2 diabetes, which result from inadequate production insulin the islet. Transforming growth factor β (TGF-β) signaling essential for fetal development islets. In this study, we exposed HIT-T15, a clonal line, TGF-β signaling. We found that inhibition promotes proliferation cells significantly, while...
Objective The main objective of this study was to evaluate the effect different feeding levels a total mixed ration silage-based diet on feed intake, tract digestion, enteric methane emissions, and energy partitioning in two beef cattle genotypes. Methods Six mature bulls (three Thai natives, three natives - Charolais crossbreeds) were assigned replicated 3×3 Latin square design, with breed genotype separate squares, periods 21 days, above maintenance (1.1, 1.5, 2.0 MEm, where MEm is...
To assess temporal changes to the risk of death in COVID-19 cases caused by Omicron variant, we calculated age-standardized case fatality rates (CFR) patients aged ≥40 years over nine diagnostic periods (3 January 28 August 2022) ten Japanese prefectures (14.8 million residents). Among 552,581 study subjects, found that there were 1836 fatalities during isolation period (up days from date onset). The highest CFR (0.85%, 95% confidence interval (CI):0.78-0.92) was observed diagnosed second...
Abstract This study proposed a method for measuring the methane (CH 4 )/carbon dioxide (CO 2 ) ratio from individual beef cattle under on‐farm conditions and estimated variance components of CH /CO ratio. Gas measurements were conducted using 166 Japanese Black group‐housed in pens equipped with feed bins. The gas containing animal's breath was measured individually after concentrate feeding by covering bin sheet sampling inlets. Measurements performed six times (three consecutive days,...
Abstract: From January 2007, we started to perform the tonsillectomy for every patient with recurrent IgA nephropathy (IgAN) after kidney transplantation. Up September 2008, four recipients primary IgAN had biopsy‐proven IgAN. They also progressive hematuria or proteinuria from on average 14.3 months Then their specimens diagnosed as were collected and they underwent tonsillectomies 52.3 Abnormal urinary findings of all patients favorably improved tonsillectomy. All cases but one mild renal...
Induced pluripotent stem cells (iPSCs) have been proposed as novel cell sources for genetic disease models and revolutionary clinical therapies. Accordingly, human iPSC-derived cardiomyocytes are potential cardiomyocyte transplantation therapy. We previously developed a generation method peripheral T cell-derived iPSCs (TiPSCs) that uses minimally invasive approach to obtain patient cells. However, it remained unknown whether TiPSCs with genomic rearrangements in the receptor (TCR) gene...
We evaluated the chemical compositions of cassava pulp samples produced by four starch factories at different locations in northeast Thailand and metabolizable energy (ME) for cattle. There were significant differences P (0.03% ± 0.02%) K (0.36% 0.2%) contents from (p < .05). Moreover, we found interactions between factory season neutral detergent fiber (36.02% 8.8%) nonfibrous carbohydrate (59.33% 9.1%) For evaluation ME, a crossover-design experiment with cattle maintained each two groups...