A5103099630- Cardiac electrophysiology and arrhythmias
- Cardiac Arrhythmias and Treatments
- Cardiac pacing and defibrillation studies
- Cardiac Ischemia and Reperfusion
- Atrial Fibrillation Management and Outcomes
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Ion channel regulation and function
- Cardiac Arrest and Resuscitation
- Eosinophilic Disorders and Syndromes
- Cardiac Imaging and Diagnostics
- Chronic Myeloid Leukemia Treatments
- Fuel Cells and Related Materials
- ECG Monitoring and Analysis
- Anesthesia and Neurotoxicity Research
- Platelet Disorders and Treatments
- Cardiovascular Function and Risk Factors
- Mitochondrial Function and Pathology
- Heart Rate Variability and Autonomic Control
- Adipose Tissue and Metabolism
- Adenosine and Purinergic Signaling
- Neuroscience and Neural Engineering
- Cardiovascular Syncope and Autonomic Disorders
- biodegradable polymer synthesis and properties
- Cytokine Signaling Pathways and Interactions
- Neurological disorders and treatments
Kyorin University
2014-2024
EA Pharma (Japan)
2020-2024
Shijonawate Gakuen University
2023
Kyorin University Hospital
2015-2022
Keio University
2003-2021
Chiba University
2001-2018
Okayama Medical Center
2016-2018
Kinan Hospital
2017
St. Marianna University School of Medicine
2015
Nippon Medical School
2015
Although somatic stem cells have been reported to exist in various adult organs, there few reports concerning the heart. We here demonstrate that Sca-1-positive (Sca-1+) hearts some of features cells. Sca-1+ were isolated from murine by a magnetic cell sorting system and cultured on gelatin-coated dishes. A fraction stuck culture dish proliferated slowly. When treated with oxytocin, expressed genes cardiac transcription factors contractile proteins showed sarcomeric structure spontaneous...
Studies on patients and large animal models suggest the importance of atrial fibrosis in development fibrillation (AF). To investigate whether increased is sufficient to produce a substrate for AF, we have studied cardiac electrophysiology (EP) inducibility arrhythmias MHC-TGFcys 33 ser transgenic mice (Tx), which atrium but not ventricles. In anesthetized mice, wild-type (Wt) Tx did show significant differences surface ECG parameters. With transesophageal pacing, no were observed EP...
Provocation tests of coronary artery spasm are useful for the diagnosis vasospastic angina (VSA). However, these thought to have a potential risk arrhythmic complications, including ventricular tachycardia (VT), fibrillation (VF), and brady-arrhythmias. We aimed elucidate safety clinical implications provocation in nationwide multicentre registry study by Japanese Coronary Spasm Association. A total 1244 VSA patients (M/F, 938/306; median 66 years) who underwent were enrolled from 47...
Background —Pharmacological evidence has implicated ATP-sensitive K + (K ATP ) channels as the effectors of cardioprotection, but relative roles mitochondrial (mitoK and sarcolemmal (surfaceK remain controversial. Methods Results —We examined effects channel blocker HMR1098 opener P-1075 on surfaceK mitoK in rabbit ventricular myocytes. (30 μmol/L) inhibited current activated by metabolic inhibition, whereas drug did not blunt diazoxide (100 μmol/L)-induced flavoprotein oxidation, an index...
OBJECTIVES To determine the mechanism of cardioprotection afforded by nicorandil, an orally efficacious antianginal drug, we examined its effects on ATP-dependent potassium (KATP) channels. BACKGROUND Nicorandil can mimic ischemic preconditioning, while mitochondrial KATP(mitoKATP) channels rather than sarcolemmal KATP(surfaceKATP) have emerged as likely effectors. METHODS Flavoprotein fluorescence and membrane current in intact rabbit ventricular myocytes were measured simultaneously to...
The large-conductance Ca2+-activated K+ (BK(Ca)) channel in the cardiac inner mitochondrial membrane (mitoK(Ca) channel) has been shown to protect heart against ischemic injury. However, questions about cardioprotective mechanism and kinase-mediated regulation of mitoK(Ca) channels remain be answered.Flavoprotein fluorescence guinea pig ventricular myocytes was measured assay activity. Ca2+ concentration ([Ca2+]m) potential (DeltaPsi(m)) were by loading cells with rhod-2 JC-1, respectively....
Coronary artery spasm plays an important role in the pathogenesis of ischemic heart disease; however, its sudden cardiac death remains to be fully elucidated. We examined clinical characteristics and outcomes patients with vasospastic angina (VSA) our nationwide multicenter registry by Japanese Spasm Association.Between September 2007 December 2008, 1429 VSA (male/female, 1090/339; median, 66 years) were identified. They characterized a high prevalence smoking included 35 who survived...
Long QT syndrome (LQTS) is an inheritable and life-threatening disease; however, it often difficult to determine disease characteristics in sporadic cases with novel mutations, more precise analysis necessary for the successful development of evidence-based clinical therapies. This study thus sought better characterize ion channel cardiac disorders using induced pluripotent stem cells (iPSCs). We reprogrammed somatic from a patient LQTS controls, differentiated them into cardiomyocytes...
AimsAtrial fibrillation (AF) is a common arrhythmia frequently associated with hypertension. This study was designed to test the hypothesis that lowering blood pressure by angiotensin II-receptor blockers (ARB) has more beneficial effects than conventional calcium channel (CCB) on frequency of paroxysmal AF
Current expert consensus recommends remote monitoring for cardiac implantable electronic devices, with at least annual in-office follow-up. We studied safety and resource consumption of exclusive follow-up (RFU) in pacemaker patients 2 years.In Japan, consecutive committed to were randomized either RFU or conventional (conventional follow-up) twice yearly intervals. only seen if indicated by monitoring. All returned hospital after years. The primary end point was a composite death, stroke,...
Patients diagnosed with high-risk essential thrombocythemia (ET) have limited treatment options to reduce the risk of thrombosis and lessen progression disease by targeting molecular source. Hydroxyurea is recommended treatment, but many patients experience resistance or intolerance. Anagrelide an approved second-line option for ET, concerns a higher frequency transformation may affect its role as suitable long-term option. Interferons been evaluated in myeloproliferative neoplasms over 30...
Background: Polycythemia vera (PV) is a myeloproliferative neoplasm. Ropeginterferon alfa-2b new-generation polyethylene glycol-conjugated proline-interferon. It approved for the treatment of PV at starting dose 100 µg (50 patients receiving hydroxyurea (HU)) and titrations up to 500 by 50 increments. The study was aimed assessing its efficacy safety higher simpler intra-patient escalation. Methods: Forty-nine with having HU intolerance from major hospitals in China were treated biweekly an...
Background— We recently demonstrated that the sarcolemmal ATP-sensitive potassium (sarcK ATP ) channel plays a key role in cardioprotection against ischemia/reperfusion injuries Kir6.2-knockout (KO) mice. In present study, we evaluated effects of diazoxide, mitochondrial (mitoK opener, on ischemia-induced myocardial stunning sarcK channel-deficient Methods and Results— Langendorff-perfused hearts wild-type (WT) KO mice were subjected to global ischemia/reperfusion. Diazoxide improved...
Background: A multicenter, randomized, placebo-controlled, double-blind trial was conducted with patients persistent atrial fibrillation (AF) to determine the dose-response effects and safety of bepridil, using every-day transtelephonic monitorings. Methods Results: total 90 were randomized receive placebo, 100 mg/day 200 bepridil treatment for 12 weeks. After treatment, those who converted sinus rhythm 3.4% in 37.5% received 69.0% mg/day, thus demonstrating a linear relationship AF...
We tested whether opening of mitochondrial ATP-sensitive K + (mitoK ATP ) channels depolarizes membrane potential (ΔΨ m and thereby prevents the Ca 2+ overload. With use a Nipkow disk confocal system, concentration ([Ca ] ΔΨ in rat ventricular myocytes were measured by loading cells with Rhod-2 JC-1, respectively. Exposure to ouabain (1 mmol/L) for 30 minutes produced overload, intensity fluorescence significantly increased 173±16% baseline ( P <0.001). Treatment mitoK channel opener...
The activation of cardiac ATP-sensitive potassium channels is reported to protect myocardium during ischemia. However, the behavior and role this channel reperfusion remain uncertain.Guinea pig right ventricular walls were studied by use microelectrodes a force transducer. Each preparation was perfused via coronary artery at constant flow rate stimulated 3 Hz. In first protocol, subjected 10 minutes no-flow ischemia, which followed 60 reperfusion. Introduction ischemia shortened action...
Several studies showed that Sf-9 cells can synthesize the galactosylated N-linked oligosaccharides if β-1,4-galactosyltransferase (β-1,4-GalT) is supplied. The full-length human β-1,4-GalT I, II, III, IV, V, and VI cDNAs were independently transfected into cells, galactosylation of endogenous membrane glycoproteins was examined by lectin blot analysis using Ricinus communis agglutinin-I (RCA-I), which preferentially interacts with terminated Galβ1→4GlcNAc group. RCA-I-reactive bands appeared...