A5057187893- Neuropeptides and Animal Physiology
- Biochemical Analysis and Sensing Techniques
- Pancreatic function and diabetes
- S100 Proteins and Annexins
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- Cellular transport and secretion
- Galectins and Cancer Biology
- Clinical Nutrition and Gastroenterology
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Cell Adhesion Molecules Research
- Diet and metabolism studies
- Digestive system and related health
- Olfactory and Sensory Function Studies
- Neonatal Respiratory Health Research
- Adipose Tissue and Metabolism
- T-cell and B-cell Immunology
- Hypothalamic control of reproductive hormones
- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Pain Mechanisms and Treatments
- Peptidase Inhibition and Analysis
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neuroendocrine Tumor Research Advances
Environmental Restoration and Conservation Agency
2024
Hokkaido University
2014-2023
Keio University
1998-2022
Functional Technologies (United Kingdom)
2022
Graduate School USA
2013
Institute of Cytology
2013
Sapporo Medical University
2012
University of Southampton
2012
Daido University
2009
Iwate University
2008
OBJECTIVE The significant roles of brown adipose tissue (BAT) in the regulation energy expenditure and adiposity are established small rodents but have been controversial humans. objective is to examine prevalence metabolically active BAT healthy adult humans clarify effects cold exposure adiposity. RESEARCH DESIGN AND METHODS In vivo 2-[18F]fluoro-2-deoxyglucose (FDG) uptake into was measured 56 volunteers (31 male 25 female subjects) aged 23–65 years by positron emission tomography (PET)...
Brown adipose tissue (BAT) burns fat to produce heat when the body is exposed cold and plays a role in energy metabolism. Using fluorodeoxyglucose-positron emission tomography computed tomography, we previously reported that BAT decreases with age thereby accelerates age-related accumulation of humans. Thus, recruitment may be effective for reduction. In this study, examined effects repeated stimulation by capsinoids (nonpungent capsaicin analogs) healthy human subjects low activity. Acute...
ATP-sensitive K+ (KATP) channels regulate many cellular functions by linking cell metabolism to membrane potential. We have generated KATP channel-deficient mice genetic disruption of Kir6.2, which forms the ion-selective pore channel. The homozygous (Kir6.2(-/-)) lack channel activity. Although resting potential and basal intracellular calcium concentrations ([Ca2+]i) pancreatic beta cells in Kir6.2(-/-) are significantly higher than those control (Kir6.2(+/+)), neither glucose at high nor...
Abstract We have revealed that 100–200 clusters, filled with closely packed lymphocytes, can be found throughout the length of antimesenteric wall mouse small intestine. They are composed a large B cell area, including germinal center, and epithelia overlying clusters contain M cells. A fraction cells displays B220+CD19+CD23+IgMlowIgDhighCD5−Mac-1− phenotype, composition IgA+ is smaller but substantial. To our knowledge, these first identification isolated lymphoid follicles (ILF) in ILF...
Cryptopatches (CPs) are part of the murine intestinal immune compartment. Cells isolated from CPs small intestine that were c-kit positive (c-kit + ) but lineage markers negative (Lin − gave rise to T cell receptor (TCR) αβ and TCR γδ intraepithelial cells after in vivo transfer or tissue engraftment into severe combined immunodeficient mice. In contrast, Peyer's patches mesenteric lymph nodes, which belong same compartment lack Lin cells, failed do so. These findings results electron...
Although several studies have suggested that insulin-secreting cells can be generated in vitro from residing adult exocrine pancreas, neither the origin of these nor their precise insulin secretory properties was obtained. We show here derived mouse pancreatic by suspension culture presence EGF and nicotinamide. The frequency insulin-positive only 0.01% initial preparation increased to ≈5% conditions. Analysis Cre/loxP-based direct cell lineage tracing system indicates newly made originate...
Intracellular homeostasis for zinc is achieved through the coordinate regulation of specific transporters engaged in influx, efflux, and intracellular compartmentalization. We have identified a novel mammalian transporter, transporter 5 (ZnT-5), by virtue its similarity to ZRC1, <i>Saccharomyces cerevisiae</i>, member cation diffusion facilitator family. Human ZnT-5 (hZnT-5) cDNA encodes 765-amino acid protein with 15 predicted membrane-spanning domains. hZnT-5 was ubiquitously expressed all...
The putative membrane fatty acid transporter (FAT) protein and its mRNA, originally expressed in adipose tissue, were found the tongue of rats. Northern blot analysis showed a significant expression FAT mRNA epithelial layer circumvallate papillae. Immunohistochemical staining revealed that immunoreactivity for is specifically localized apical part taste bud cells, possibly gustatory
Although the excitatory amino acid glutamate and its receptors play crucial roles in many functions of central nervous system (CNS), their presence peripheral tissues has remained unclear. In present study, we have identified kainate, α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), N-methyl-D-aspartate (NMDA) receptor subtype mRNAs pancreatic islets, using reverse transcriptase polymerase chain reaction (RT-PCR). Intracellular calcium ([Ca2+]i) measurements electrophysiological...
We have previously shown that cAMP-binding protein cAMP-guanidine nucleotide exchange factor II (GEFII) (or Epac2) interacting with Rim2 is involved in cAMP-dependent, kinase A-independent exocytosis pancreatic β-cells. The action of the cAMP-GEFII·Rim2 complex requires both intracellular cAMP and Ca<sup>2+</sup>. Although has C<sub>2</sub>domains, its role as a Ca<sup>2+</sup> sensor remained unclear. In present investigation, we discovered Piccolo, CAZ (cytoskeletal matrix associated...
We investigated the expression of G protein-coupled receptor GPR40 and GPR120 in rat tongue. Using reverse transcription polymerase chain reaction, we detected a significant mRNA epithelium circumvallate papillae but not nonsensory epithelium, while was undetectable sensory papillae. Western blotting analysis colon for showed protein band with molecular weight that corresponds to GPR120, indicating this antibody could recognize native form GPR120. Immunohistochemistry using anti-GPR120...
Graphene oxide (GO) consisting of a carbon monolayer has been widely investigated for tissue engineering platforms because its unique properties. For this study, we fabricated GO-applied scaffold and assessed the cellular behaviors in scaffold. A preclinical test was conducted to ascertain whether GO promoted bone induction dog tooth extraction sockets. scaffolds were prepared by coating surface collagen sponge with 0.1 1 µg/mL dispersion. Scaffolds characterized using scanning electron...
Zinc transporters play a critical role in spatiotemporal regulation of zinc homeostasis. Although disruption homeostasis has been implicated disorders such as intestinal inflammation and aberrant epithelial morphology, it is largely unknown which are responsible for the Here, we show that Zrt-Irt-like protein (ZIP) transporter ZIP7, highly expressed crypt, essential proliferation. Mice lacking Zip7 epithelium triggered endoplasmic reticulum (ER) stress proliferative progenitor cells, leading...
ATP-sensitive K + (K ATP ) channels are known to play important roles in various cellular functions, but the direct consequences of disruption channel function largely unknown. We have generated transgenic mice expressing a dominant-negative form subunit Kir6.2 (Kir6.2G132S, substitution glycine with serine at position 132) pancreatic beta cells. Kir6.2G132S develop hypoglycemia hyperinsulinemia neonates and hyperglycemia hypoinsulinemia decreased cell population adults. is found be impaired...
1. The aim of our study is to clarify the relationship between expression pattern P2X receptors and cell type male adult rat (Wistar) dorsal root ganglion (DRG) neurons. We identified nociceptive cells acutely dissociated DRG neurons from rats using capsaicin sensitivity. 2. Two types ATP-activated currents, one with fast, other slow desensitization, were found under voltage-clamp conditions. In addition, fast but not desensitization responded capsaicin, indicating that there was a current...