A5074494609- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- Mechanisms of cancer metastasis
- Signaling Pathways in Disease
- Cancer Mechanisms and Therapy
- NF-κB Signaling Pathways
- Rheumatoid Arthritis Research and Therapies
- Cancer-related Molecular Pathways
- Ferroptosis and cancer prognosis
- Acute Myeloid Leukemia Research
- Cancer Treatment and Pharmacology
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Protein Kinase Regulation and GTPase Signaling
- Colorectal Cancer Treatments and Studies
- Cancer, Lipids, and Metabolism
- Lipid metabolism and biosynthesis
- interferon and immune responses
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- T-cell and B-cell Immunology
- Telomeres, Telomerase, and Senescence
- Blood Coagulation and Thrombosis Mechanisms
University of Massachusetts Chan Medical School
2011-2023
Showa Denko (Japan)
2021
The University of Tokyo
2009-2016
National Cancer Centre Japan
2015
National Cancer Research Institute
2004-2006
Yokohama City University
1999-2004
Peptidylarginine deiminase (PAD) deiminates arginine residues in proteins to citrulline Ca2+ dependently. There are four types of PADs, I, II, III, and V, humans. We studied the subcellular distribution PAD V HL-60 granulocytes peripheral blood granulocytes. Expression green fluorescent protein-tagged PADs HeLa cells revealed that is localized nucleus, whereas III cytoplasm. deletion mutants indicated sequence 45–74 have a nuclear localization signal (NLS). A feature this NLS three-lysine...
Three types of peptidylarginine deiminase (PAD), which converts a protein arginine residue to citrulline residue, are widely distributed in animal tissues. Little is known about PAD hemopoietic cells. We found that activity human myeloid leukemia HL-60 cells was induced with the granulocyte-inducing agents retinoic acid and dimethyl sulfoxide monocyte-inducing agent 1α,25-dihydroxyvitamin D3. cloned characterized cDNA from acid-induced The 2,238 base pairs long encoded 663-amino polypeptide....
Peptidylarginine deiminase, registered as PAD V in the DDBJ/GenBank/EMBL data banks, is expressed HL-60 cells differentiated into granulocytes or monocytes. We analyzed activities density-fractionated human peripheral blood cell fractions. activity with similar substrate specificity to that of was found eosinophil and neutrophil fractions, which showed single bands comigrating authentic on immunoblotting an anti-PAD antibody. Both biochemical analyses marked enrichment fraction. Its...
Abstract Colon cancers have been shown to develop after accumulation of multiple genetic and epigenetic alterations with changes in global gene expression profiles, contributing the establishment widely diverse phenotypes. Transcriptional posttranscriptional regulation by small RNA species, such as interfering microRNA RNA-induced silencing complex (RISC), is currently drawing major interest regard cancer development. SND1, also called Tudor-SN p100 recently reported be a component RISC,...
Natural immunoglobulin M (IgM) is reactive to autoantigens and believed be important for autoimmunity. Blood pentameric IgM loaded with antigens forms a large immune complex (IC) that contains various elements, including apoptosis inhibitor of macrophage (AIM). Here we demonstrate this IgM-AIM association contributes autoantibody production under obese conditions. In mice fed high-fat diet, natural increased through B cell TLR4 stimulation. AIM associated protected from renal excretion,...
Involvement of Ras in cancer initiation is known, but recent evidence indicates a role progression, including metastasis and invasion; however, the mechanism still unknown. In this study, it was determined that human lung cells with mutations, among other popular showed significantly higher expression CUB domain-containing protein 1 (CDCP1) than those without. Furthermore, activated clearly induced CDCP1, whereas CDCP1 knockdown or inhibition phosphorylation by Src-directed therapy abrogated...
Peptidylarginine deiminase 4 (PAD4) functions as a transcriptional coregulator by catalyzing the conversion of histone H3 arginine residues to citrulline residues. Although high level PAD4 expression in bone marrow cells suggests its involvement haematopoiesis, precise contribution remains unclear. Here we show that PAD4, which is highly expressed lineage− Sca-1+ c-Kit+ (LSK) mouse compared with other progenitor cells, controls c-myc citrullination on promoter. Furthermore, associated...
Abstract The conventional planar culture of adherent cells is inefficient for large-scale manufacturing cell and gene therapy products. We developed a facile efficient bead-to-bead cell-transfer method serial subculture expansion human mesenchymal stem (hMSCs) with microcarriers in bioreactors. first compared medium without nucleosides found the former maintained expression surface markers hMSCs during their prolonged enabled faster proliferation. Subsequently, we our transfer to that...
CUB domain‐containing protein‐1 ( CDCP 1) is a trans‐membrane protein predominantly expressed in various cancer cells and involved tumor progression. 1 phosphorylated at tyrosine residues the intracellular domain by Src family kinases recruits PKC δ to plasma membrane through phosphorylation‐dependent association with C2 of δ, which turn induces survival signal an anchorage‐independent condition. In this study, we used our cell‐free screening system identify small compound, glycoconjugated...
Apoptosis inhibitor of macrophage (AIM, encoded by cd5l) is a multi-functional circulating protein that has beneficial role in the regulation broad range diseases, some which are ameliorated AIM administration mice. In blood, stabilized association with IgM pentamers and maintains its high levels. The mechanism regulating excessive accumulation blood remains unknown, although it important, since constitutive increase levels promotes chronic inflammation. Here we found physiological...