A5002282550- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Dialysis and Renal Disease Management
- Enzyme Structure and Function
- Ion Transport and Channel Regulation
- Plant Virus Research Studies
- Bacterial Genetics and Biotechnology
- Fibroblast Growth Factor Research
- Parathyroid Disorders and Treatments
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Erythropoietin and Anemia Treatment
- Plant Molecular Biology Research
- Ion channel regulation and function
- Bacteriophages and microbial interactions
- Plant Reproductive Biology
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Protein Structure and Dynamics
- Plant and Fungal Interactions Research
- Cardiac Ischemia and Reperfusion
- Proteoglycans and glycosaminoglycans research
- Electrolyte and hormonal disorders
- Plant-Microbe Interactions and Immunity
Otsuma Women's University
2021-2025
Oita University
2005-2025
Toyo University
2013-2025
Hirosaki University Hospital
2024
Nagoya University
2001-2024
Nagoya University Hospital
2023-2024
Shinrakuen Hospital
2010-2023
Tsuchiura Kyodo General Hospital
2023
Oita University Hospital
2001-2023
Yokohama National University
2004-2022
Four inherited neurodegenerative diseases are linked to abnormally expanded repeats of glutamine residues in the affected proteins. Molecular modeling followed by optical, electron, and x-ray diffraction studies a synthetic poly(L-glutamine) shows that it forms beta-sheets strongly held together hydrogen bonds. Glutamine may function as polar zippers, for example, joining specific transcription factors bound separate DNA segments. Their extension cause disease either increased, nonspecific...
The development of platelet-rich fibrin (PRF) drastically simplified the preparation procedure platelet-concentrated biomaterials, such as plasma (PRP), and facilitated their clinical application. PRF's effectiveness has often been demonstrated in pre-clinical studies; however, it is still controversial whether growth factors are significantly concentrated PRF preparations to facilitate wound healing tissue regeneration. To address this matter, we performed a comparative study factor...
Recently it has been postulated that mitochondrial ATP-sensitive K+ (mitoKATP) channels rather than sarcolemmal KATP (sarcKATP) are important as end effectors and/or triggers of ischemic preconditioning (IPC). To define the pathophysiological significance sarcKATP channels, we conducted functional experiments using Kir6.2-deficient (KO) mice. Metabolic inhibition with glucose-free, dinitrophenol-containing solution activated current and shortened action potential duration in ventricular...
Fibroblast growth factor (FGF) 21, a structural relative of FGF23 that regulates phosphate homeostasis, is regulator insulin-independent glucose transport in adipocytes and plays role the regulation body weight. It also ketogenesis adaptive responses to starvation. We report reconstituted receptor activation assay system using BaF3 cells, which do not endogenously express any type FGF (FGFR) or heparan sulfate proteoglycan, FGF21 alone does activate FGFRs betaKlotho required for two specific...
Abstract The Japanese Society for Dialysis Therapy (JSDT) guideline committee, chaired by Dr Y. Tsubakihara, presents the guidelines entitled “Guidelines Renal Anemia in Chronic Kidney Disease.” These replace “2004 JSDT Guidelines Hemodialysis Patients,” and contain new, additional peritoneal dialysis (PD), non‐dialysis (ND), pediatric chronic kidney disease (CKD) patients. Chapter 1 reference values diagnosing anemia that are based on most recent epidemiological data from general...
A novel phosphorylation-dependent inhibitory protein (IP) of porcine aorta myosin light chain phosphatase (PA-MLCP) was purified to homogeneity from media. The molecular mass IP 20 kDa. phosphorylated by endogenous potentiating kinase (IP-K) inhibited not only PA-MLCP activity, but also that the catalytic subunit phosphatase-1. amino acid sequence a peptide derived with IP-K, RHARVT*VK, shared one consensus sequences phosphorylatable C (PKC), where T* phosphorylated. PKC and product as strongly IP-K.
Recently it has been postulated that mitochondrial ATP-sensitive K+ (mitoKATP) channels rather than sarcolemmal KATP (sarcKATP) are important as end effectors and/or triggers of ischemic preconditioning (IPC). To define the pathophysiological significance sarcKATP channels, we conducted functional experiments using Kir6.2-deficient (KO) mice. Metabolic inhibition with glucose-free, dinitrophenol-containing solution activated current and shortened action potential duration in ventricular...
Repressor-activator protein 1 (RAP1) has an essential role in the maintenance of yeast telomeres. Yeast telomeric DNA consists simple repeated G-rich sequences that are bound by RAP1. We have found RAP1, addition to its known binding activity for double-stranded DNA, interacts with strand containing guanine base (G)-tetrads. show here using circular dichroism spectroscopy RAP1 promotes formation one particular type quadruplex, parallel G4-DNA. Furthermore, is able bind both preformed and...
Abstract —ATP-sensitive potassium (K ATP ) channels were discovered in ventricular cells, but their roles the heart remain mysterious. K have also been found numerous other tissues, including vascular smooth muscle. Two pore-forming subunits, Kir6.1 and Kir6.2, contribute to diversity of channels. To determine which subunits are operative cardiovascular system functional roles, we characterized effects pharmacological + channel openers (KCOs, ie, pinacidil, P-1075, diazoxide)...
Pretreatment with a combination of granulocyte colony-stimulating factor (G-CSF) and stem cell (SCF) has been reported to attenuate left ventricular (LV) remodeling after acute myocardial infarction (MI). We here examined whether the cytokine treatment started MI also beneficial effects. Anterior was created in recipient mice whose bone marrow had replaced that transgenic expressing enhanced green fluorescent protein (GFP). categorized into five groups according following treatment: 1)...
The differences between the biosynthesis of sterols in higher plants and yeast/mammals are believed to originate at cyclization step oxidosqualene, which is cyclized cycloartenol lanosterol yeast/mammals. Recently, synthase genes were identified from dicotyledonous plant species including Arabidopsis, suggesting that possess dual biosynthetic pathways phytosterols via lanosterol, through cycloartenol. To identify pathway phytosterol reveal contributions each we performed feeding experiments...
Summary 3‐Hydroxy‐3‐methylglutaryl‐CoA reductase (HMGR) catalyzes the first committed step in cytosolic isoprenoid biosynthesis pathway higher plants. To understand contribution of HMGR to plant development, we isolated T‐DNA insertion mutants for HMG1 and HMG2 . The hmg1 hmg2 were both more sensitive than wild type (WT) lovastatin, an inhibitor HMGR. mutant showed no visible phenotype under normal growth conditions. In contrast, exhibited dwarfing, early senescence, sterility. Expression...
Expressed sequence tag (EST) sequencing has proven to be an economically feasible alternative for gene discovery in species lacking a draft genome sequence. Ongoing large-scale EST projects feel the need bioinformatics tools facilitate uniform handling. This brings about renewed importance universal tool processing and functional annotation of large sets ESTs. EGassembler ( http://egassembler.hgc.jp/ ) is web server, which provides automated as well user-customized analysis cleaning, repeat...
Homozygous mutations in SLC4A4 , encoding the electrogenic Na + -HCO 3 − cotransporter NBCe1, have been known to cause proximal renal tubular acidosis (pRTA) and ocular abnormalities. In this study, we report two sisters with pRTA, abnormalities, hemiplegic migraine. Genetic analysis ruled out pathological genes for familial migraine, but identified a homozygous 65-bp deletion (Δ65bp) C terminus of corresponding codon change S982NfsX4. Several heterozygous members family also presented...
Sodium transport through various nephron segments is quite important in regulating sodium reabsorption and blood pressure. Among several regulators of this process, insulin acts on almost all the a strong enhancer reabsorption. Sodium-proton exchanger type 3 (NHE3) main regulator luminal side proximal tubule. In basolateral tubule, sodium-bicarbonate cotransporter (NBCe1) mediates bicarbonate exit from tubular cells. distal connecting epithelial channel (ENaC) great importance to NHE3,...