A5063062046- Urinary Bladder and Prostate Research
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Ion Channels and Receptors
- Gastrointestinal motility and disorders
- Ion Transport and Channel Regulation
- Nitric Oxide and Endothelin Effects
- Gastroesophageal reflux and treatments
- Neuroscience of respiration and sleep
- Connexins and lens biology
- Pain Mechanisms and Treatments
- Neuroendocrine regulation and behavior
- Diet and metabolism studies
- Pelvic floor disorders treatments
- Infant Health and Development
- Helicobacter pylori-related gastroenterology studies
- Advanced Glycation End Products research
- Cardiac electrophysiology and arrhythmias
- Barrier Structure and Function Studies
- Kidney Stones and Urolithiasis Treatments
- Receptor Mechanisms and Signaling
- Urological Disorders and Treatments
- Adenosine and Purinergic Signaling
- Microencapsulation and Drying Processes
- Proteoglycans and glycosaminoglycans research
Nagoya City University
2015-2024
Mizuho (Japan)
2015-2024
Waseda University
2002-2011
Nihon University
2010
Institute for Environmental Sciences
2005-2006
University of Shizuoka
2005-2006
Shizuoka University
2006
Abstract Short‐chain fatty acids (SCFAs), including propionate, butyrate and acetate, are fermentation products of carbohydrates in the colon. We investigated contractile effects SCFAs on rat distal Mechanical activity circular muscle strip preparations was recorded vitro . Propionate concentration‐dependently (10 μ mol L −1 –10 mmol ) induced rapid, large amplitude phasic contractions (the first phase) followed by tonic second phase). Acetate itself had no effect activity, although...
Abstract During the urine storage phase, tonically contracting urethral musculature would have a higher energy consumption than bladder muscle that develops phasic contractions. However, ischaemic dysfunction is less prevalent in urethra bladder, suggesting vasculature has intrinsic properties ensuring an adequate blood supply. Diameter changes rat or mouse arterioles were measured using video‐tracking system. Intercellular Ca 2+ dynamics arteriolar smooth (SMCs) and endothelial cells...
We investigated the properties of suburothelial microvessels, which have a vital role in maintaining microcirculation to cells involved bladder afferent signaling.Changes diameter rat microvessels were measured using video microscopy. Membrane potential changes and intracellular Ca(2+) dynamics venules examined recording techniques imaging fluo-4 fluorescence, respectively.Suburothelial showed spontaneous action vasoconstriction activity while arterioles quiescent. Venular was prevented by...
In suburothelial venules of rat bladder, pericytes (perivascular cells) develop spontaneous Ca(2+) transients, which may drive the smooth muscle wall to generate venular constrictions. We aimed further explore morphological and functional characteristics in mouse bladder.The features were investigated by electron microscopy fluorescence immunohistochemistry. Changes diameters measured using video microscopy, while intracellular dynamics visualized Fluo-4 imaging.A network α-smooth actin...
BACKGROUND AND PURPOSE Although atypical smooth muscle cells (SMCs) in the proximal renal pelvis are thought to generate pacemaker signals that drive pyeloureteric peristalsis, their location and electrical properties remain obscure. EXPERIMENTAL APPROACH Standard patch clamp, intracellular microelectrode immunohistochemistry techniques were used. To unequivocally identify SMCs, transgenic mice with enhanced yellow fluorescent protein (eYFP) expressed containing α‐smooth actin (α‐SMA)...
Slow waves (slow ICC ) were recorded from myenteric interstitial cells of Cajal (ICC-MY) in situ the rabbit small intestine, and their properties compared with those mouse intestine. Rabbit slow consisted an upstroke depolarization followed by a distinct plateau component. Ni 2+ nominally Ca -free solutions reduced rate-of-rise amplitude depolarization. Replacement Sr enhanced component but decreased . In contrast, replacing both components The was inhibited low-extracellular-Cl −...
We investigated the neurohumoral modulation of contractility bladder muscularis mucosae (mucosa) compared with that detrusor smooth muscle.Changes in mucosal and bundles from guinea pig bladders were measured using isometric tension recording. The morphological relationship between blood vessels, their sensory innervation was examined by fluorescence immunohistochemistry.Meshworks numerous branches anastomosis preferentially ran parallel suburothelial vessels. Although PTHrPRs (parathyroid...
Key points In the bladder suburothelial microvasculature, pericytes in different microvascular segments develop spontaneous Ca 2+ transients with or without associated constrictions. Spontaneous of all primarily rely on cycles uptake and release by sarco‐ endoplasmic reticulum. The synchrony capillary exclusively relies spread depolarizations resulting from opening ‐activated chloride channels (CaCCs) via gap junctions. CaCC‐dependent further activate L‐type voltage‐dependent as required for...
We investigated the cellular mechanisms underlying spontaneous contractions in mouse renal pelvis, regulated by calcitonin gene-related peptide (CGRP).Spontaneous contractions, action potentials and Ca2+ transients typical atypical smooth muscle cells (TSMCs ATSMCs) within pelvis wall were recorded separately using tension intracellular microelectrode recording techniques Fluo-4 imaging. Immunohistochemical electron microscopic studies also carried out.Bundles of CGRP containing transient...
Venules within the gut wall may have intrinsic mechanisms for maintaining circulation even upon intestinal distension. We aimed to explore spontaneous and nerve-mediated contractile activity of colonic venules.Changes in diameter submucosal venules rat distal colon were measured using video microscopy. The innervation microvasculature was investigated fluorescence immunohistochemistry.Submucosal exhibited constrictions that abolished by blockers L-type Ca(2+) channels (1 μM nicardipine),...
Abstract Background Constipation is commonly seen in patients with Parkinson's disease associated a loss of dopaminergic neurons both central and enteric nervous systems. However, the roles developing constipation remain to be elucidated. Here, we investigated generation colonic peristalsis. Methods Cannulated segments rat proximal colon were situated organ bath, abluminally perfused physiological salt solution luminally 0.9% saline. Drugs applied abluminal solution. Changes diameter along...
Short-chain fatty acids, such as propionate and acetate, are produced by a bacterial fermentation of carbohydrates in the colonic lumen. We examined effects on frequency mean amplitude spontaneous giant contractions (GCs) circular muscle strips rat distal colon with mucosa attached. An addition increased GCs for about 20 min (≥1 mm), but was decreased (≥0.1 mm). The propionate-induced increase blocked muscarinic acetylcholine receptor antagonist, atropine. In contrast, nicotinic...