A5055296224- Gastrointestinal motility and disorders
- Ion channel regulation and function
- Ion Channels and Receptors
- Infant Health and Development
- Ion Transport and Channel Regulation
- Cardiac electrophysiology and arrhythmias
- Diet and metabolism studies
- Congenital gastrointestinal and neural anomalies
- Nitric Oxide and Endothelin Effects
- Neuropeptides and Animal Physiology
- Helicobacter pylori-related gastroenterology studies
- Gastroesophageal reflux and treatments
- Urinary Bladder and Prostate Research
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Neuroendocrine regulation and behavior
- Digestive system and related health
- Reproductive Biology and Fertility
- Pelvic floor disorders treatments
- Receptor Mechanisms and Signaling
- Neuroscience and Neural Engineering
- Pain Mechanisms and Treatments
- Congenital heart defects research
- Phonocardiography and Auscultation Techniques
University of Nevada, Reno
2016-2025
University at Buffalo, State University of New York
2005-2025
Boise State University
2022
University of Huddersfield
2019
School of the Art Institute of Chicago
2017
Midwestern University
2010
Johns Hopkins Medicine
2005
Johns Hopkins University
2005
University of Alberta
2002
Queen's University
2002
1. Interstitial cells of Cajal (ICs) have been proposed as pacemakers in the gastrointestinal tract. We studied characteristics and distribution ICs electrical activity small intestinal muscles from mice with mutations at dominant-white spotting/c-kit (W) locus because tyrosine kinase function c-kit may be important development IC network. 2. W/WV mutants (days 3-30 postpartum) had few myenteric plexus region compared wild type (+/+) siblings. The present were associated neural elements lay...
The structural relationships between interstitial cells of Cajal (ICC), varicose nerve fibers, and smooth muscle in the gastrointestinal tract have led to suggestion that ICC may be involved or mediate enteric neurotransmission. We characterized distribution murine stomach found two distinct classes on basis morphology immunoreactivity antibodies against c-Kit receptors. with multiple processes formed a network myenteric plexus region from corpus pylorus. Spindle-shaped were within circular...
Interstitial cells of Cajal (ICC) are interposed between enteric neurons and smooth muscle in gastrointestinal muscles. The role intramuscular ICC (IC-IM) mediating excitatory neural inputs was studied using gastric fundus muscles wild-type animals W/W(v) mutant mice, which lack IC-IM. Excitatory motor neurons, labeled with antibodies to vesicular acetylcholine transporter or substance-P, were closely associated Immunocytochemistry showed close contacts IC-IM also formed gap junctions cells....
Interstitial cells of Cajal (ICC) generate pacemaker activity (slow waves) in gastrointestinal (GI) smooth muscles, but the mechanism(s) are controversial. Several conductances, such as Ca 2+ ‐activated Cl − channels (CaCC) and non‐selective cation (NSCC) have been suggested to be involved slow wave depolarization. We investigated expression function a new class CaCC, anoctamin 1 (ANO1), encoded by Tmem16a , which was discovered highly expressed ICC microarray screen. GI muscles express...
Patients with long-standing diabetes commonly suffer from gastric neuromuscular dysfunction (gastropathy) causing symptoms ranging postprandial bloating to recurrent vomiting. Autonomic neuropathy is generally believed be responsible for diabetic gastropathy and the underlying impairments in emptying (gastroparesis) receptive relaxation, but specific mechanisms have not been elucidated. Recently, it has recognized that interstitial cells of Cajal generate electrical pacemaker activity...
1 Pacemaker cells, known as interstitial cells of Cajal (ICC), generate electrical rhythmicity in the gastrointestinal tract. currents ICC result from activation a voltage-independent, non-selective cation conductance, but timing mechanism responsible for periodic pacemaker current is unknown. 2 Previous studies suggest that pacemaking dependent upon metabolic activity 1y1yand1 Ca2+ release intracellular stores. We tested hypothesis mitochondrial handling may underlie dependence on oxidative...
Interstitial cells of Cajal (ICC) are unique that generate electrical pacemaker activity in gastrointestinal (GI) muscles. Many previous studies have attempted to characterize the conductances responsible for current and slow waves GI tract, but precise mechanism rhythmicity is still debated. We used a new transgenic mouse with bright green fluorescent protein (copGFP) constitutively expressed ICC facilitate study these mixed cell dispersions. found express specialized 'slow wave' current....
Interstitial cells of Cajal (ICC) have been suggested as pacemaker in the gastrointestinal tract. A method was developed to isolate ICC from slow-wave region canine proximal colon. These were identified under phase-contrast microscopy, and their identity verified by comparing ultrastructure with morphology situ. Patch-clamp experiments demonstrated that these are excitable; voltage-dependent inward outward currents elicited depolarization. Inward current transients calcium currents. portion...
Electrical rhythmicity in the gastrointestinal tract may originate interstitial cells of Cajal (IC). Development IC small intestine is linked to signaling via tyrosine kinase receptor, c-kit. express c-kit protein, and disruption causes breakdown networks loss slow waves. We tested whether mutations steel factor, ligand for c-kit, affect development networks. were found region myenteric plexus (IC-MY) mice with (i.e., Sl/Sld) at 5-10 days postpartum, but these formed an abnormal network....
Abstract The gastrointestinal (GI) tract responds to a variety of stimuli through local and centrally mediated pathways. Changes in the intestinal microenvironment are sensed by vagal, spinal, intrinsic primary afferent fibers. Sensory nerve endings located close lumen GI respond pH, chemical composition lumenal contents, or distortion mucosa. Afferents within muscle layers thought be tension sensitive, whereas those myenteric plexus also changes humoral substances. Subpopulations these...
1 The gastric corpus and antrum contain interstitial cells of Cajal (ICC) within the tunica muscularis. We tested hypothesis that ICC are involved in generation regeneration electrical slow waves. 2 Normal, postnatal development wave activity was characterized tissues freshly removed from animals between birth day 50 (D50). Slow amplitude frequency increased during this period. Networks myenteric (IC-MY) were present muscles at did not change significantly appearance period study as imaged...
Smooth muscles, as in the gastrointestinal tract, are composed of several types cells. Gastrointestinal muscles contain smooth muscle cells, enteric neurons, glial immune and various classes interstitial One type cell, referred to 'fibroblast-like cells' by morphologists, common, but their function is unknown. These cells found near terminals motor suggesting they could have a role generating neural responses that help control movements. We used novel mouse with bright green fluorescent...
Peripheral inhibitory nerves are physiological regulators of the contractile behavior visceral smooth muscles. One transmitters responsible for neurotransmission has been reputed to be a purine, possibly ATP. However, exact identity this substance never verified. Here we show that beta-nicotinamide adenine dinucleotide (beta-NAD), an neurotransmitter candidate, is released by stimulation enteric in gastrointestinal muscles, and pharmacological profile beta-NAD mimics endogenous better than...