A5011540072- MicroRNA in disease regulation
- Gastrointestinal motility and disorders
- Helicobacter pylori-related gastroenterology studies
- Digestive system and related health
- Genetic factors in colorectal cancer
- Diet and metabolism studies
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Ion channel regulation and function
- Gut microbiota and health
- RNA Research and Splicing
- Pancreatic function and diabetes
- Ion Channels and Receptors
- Cardiac electrophysiology and arrhythmias
- Chromosomal and Genetic Variations
- Diet, Metabolism, and Disease
- RNA and protein synthesis mechanisms
- Protein Tyrosine Phosphatases
- Metabolism, Diabetes, and Cancer
- Infant Health and Development
- Colorectal Cancer Treatments and Studies
- Congenital gastrointestinal and neural anomalies
- Sperm and Testicular Function
University of Nevada, Reno
2016-2025
Nevada System of Higher Education
2016
It is believed that depending on the thermodynamic stability of 5′-strand and 3′-strand in stem-loop structure a precursor microRNA (pre-miRNA), cells preferentially select less stable one (called miRNA or guide strand) destroy other miRNA* passenger strand). However, our expression profiling analyses revealed both strands could be co-accumulated as pairs some tissues while being subjected to strand selection tissues. Our target prediction validation assays demonstrated pair equal numbers...
Interstitial cells of Cajal (ICC) are unique that generate electrical pacemaker activity in gastrointestinal (GI) muscles. Many previous studies have attempted to characterize the conductances responsible for current and slow waves GI tract, but precise mechanism rhythmicity is still debated. We used a new transgenic mouse with bright green fluorescent protein (copGFP) constitutively expressed ICC facilitate study these mixed cell dispersions. found express specialized 'slow wave' current....
Small noncoding RNAs have been suggested to play important roles in the regulation of gene expression across all species from plants humans. To identify small expressed by ovary, we generated mouse ovarian RNA complementary DNA (srcDNA) libraries and sequenced 800 srcDNA clones. We identified 236 including 122 microRNAs (miRNAs), 79 piwi-interacting (piRNAs), 35 nucleolar (snoRNAs). Among these RNAs, 15 miRNAs, 74 piRNAs, 21 snoRNAs are novel. Approximately 70% piRNAs encoded multicopy genes...
Catsper3 and Catsper4 are two recently identified testis-specific genes homologous to Catsper1 Catsper2 that have been shown play an essential role in sperm hyperactivated motility male fertility mice. Here we report knockout mice completely infertile due a quick loss of lack under capacitating conditions. Our data demonstrate both CATSPER3 CATSPER4 required for during capacitation fertility. The present study also demands revisit the idiopathic infertility patients who show normal counts...
Transcriptome-scale data can reveal essential clues into understanding the underlying molecular mechanisms behind specific cellular functions and biological processes. Transcriptomics is a continually growing field of research utilized in biomarker discovery. The transcriptomic profile interstitial cells Cajal (ICC), which serve as slow-wave electrical pacemakers for gastrointestinal (GI) smooth muscle, has yet to be uncovered. Using copGFP-labeled ICC mice flow cytometry, we isolated...
Potassium channels activated by membrane stretch may contribute to maintenance of relaxation smooth muscle cells in visceral hollow organs. Previous work has identified K(+) murine colon that are and further regulated NO-dependent mechanisms. We have screened gastrointestinal, vascular, bladder, uterine muscles for the expression TREK TRAAK mRNA. Although TREK-1 was expressed many these muscles, TREK-2 only antrum pulmonary artery. not any tested. Whole cell currents from mammalian COS were...
We have developed a method that effectively removes all of the perinuclear materials mouse sperm head, including acrosome, plasma membrane, theca, and nuclear envelope. By injection single purified head into metaphase II oocyte followed by activation with strontium chloride, 93% zygotes two-cell embryos. Although only approximately 17% transferred embryos were born alive, live pups adults, they appeared to be normal in reproduction behavior. detected RNA species, mRNAs miRNAs from heads. Our...
Abstract Interstitial cells of Cajal (ICC) are specialized in smooth muscle organs that generate and propagate pacemaker activity, receive inputs from motor neurons, serve as mechanosensors. In the gastrointestinal tract, development maintenance ICC phenotype have been linked to intracellular signaling via Kit, but its role during embryogenesis is controversial. Here we studied functional ICC‐MY late gestational period mice. Blocking Kit with a neutralizing antibody before after spontaneous...
Genome-scale expression data on the absolute numbers of gene isoforms offers essential clues in cellular functions and biological processes. Smooth muscle cells (SMCs) perform a unique contractile function through specific genes controlled by serum response factor (SRF), transcription that binds to DNA sites known as CArG boxes. To identify SRF-regulated specifically expressed SMCs, we isolated SMC populations from mouse small intestine colon, obtained their transcriptomes, constructed an...