A5047194865- CAR-T cell therapy research
- Mast cells and histamine
- Viral Infectious Diseases and Gene Expression in Insects
- Thermoregulation and physiological responses
- Trace Elements in Health
- Nanowire Synthesis and Applications
- Virus-based gene therapy research
- Asthma and respiratory diseases
- Food Allergy and Anaphylaxis Research
- Infrared Thermography in Medicine
- Thermal Regulation in Medicine
- T-cell and Retrovirus Studies
- Diet and metabolism studies
- Atherosclerosis and Cardiovascular Diseases
- Biosimilars and Bioanalytical Methods
- Microtubule and mitosis dynamics
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Monoclonal and Polyclonal Antibodies Research
- Ion Channels and Receptors
- Congenital limb and hand anomalies
- RNA regulation and disease
- Ginkgo biloba and Cashew Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- PI3K/AKT/mTOR signaling in cancer
Shinshu University
1996-2023
RIKEN Center for Integrative Medical Sciences
2006-2019
Osaka University
2006-2011
Ube Frontier University
2011
Japan Science and Technology Agency
2009
Toxicologie, Pharmacologie et Signalisation Cellulaire
2007
Gunma University
2005-2006
University of Fukui
2006
Kitasato Institute Hospital
2006
Zinc is an essential trace element required for enzymatic activity and maintaining the conformation of many transcription factors; thus, zinc homeostasis tightly regulated. Although affects several signaling molecules may act as a neurotransmitter, it remains unknown whether acts intracellular second messenger capable transducing extracellular stimuli into events. In this study, we report that cross-linking high affinity immunoglobin E receptor (Fcε I [FcεRI]) induced release free from...
Abstract Zinc (Zn) is an essential nutrient, and its deficiency causes growth retardation, immunodeficiency, neuronal degeneration. However, the precise roles molecular mechanism(s) of Zn function in immune response have not been clarified. Mast cells (MCs) are granulated that play a pivotal role allergic reactions inflammation. The granules MCs contain various chemical mediators inflammatory cytokines released upon FcεRI cross-linking. In this study, we report for MC activation both vitro...
Zinc (Zn) is an essential nutrient and its deficiency causes immunodeficiency. However, it remains unknown how Zn homeostasis regulated in mast cells if transporters are involved allergic reactions. We show that Znt5/Slc30a5 required for contact hypersensitivity cell–mediated delayed-type response but not immediate passive cutaneous anaphylaxis. In from Znt5−/− mice, Fcε receptor I (FcεRI)–induced cytokine production was diminished, degranulation intact. Znt5 FcεRI-induced translocation of...
Recent studies have shown that zinc ion (Zn) can behave as an intracellular signaling molecule. We previously demonstrated mast cells stimulated through the high-affinity IgE receptor (FcεRI) rapidly release Zn from endoplasmic reticulum (ER), and we named this phenomenon "Zn wave". However, molecules responsible for releasing roles of wave were elusive. Here identified pore-forming α1 subunit Cav1.3 (α1D) L-type calcium channel (LTCC) gatekeeper wave. LTCC antagonists inhibited wave,...
Zinc (Zn) is an essential nutrient and its deficiency causes immunodeficiency skin disorders. Various cells including mast release Zn-containing granules when activated; however, the biological role of released Zn currently unclear. Here we report our findings that transporter ZnT2 required for from cells. In addition, found induce IL-6 production inflammatory such as fibroblasts promote wound healing, a process involves inflammation. induces variety pro-inflammatory cytokines through...
Abstract Mast cells are major players in allergic responses. IgE-dependent activation through FcεR leads to degranulation and cytokine production, both of which require Gab2. To clarify how the signals diverge at Gab2, we established Gab2 knock-in mice that express mutated either PI3K or SH2 domain-containing protein tyrosine phosphatase-2 (SHP2) binding sites. Examination these mutants showed sites were required for anaphylaxis response but not production contact hypersensitivity....
The quantitation of hepatitis C virus (HCV) viremia can be helpful in the diagnosis, therapy, and monitoring patients with chronic C. A sensitive quantitative fluorescence enzyme immunoassay (FEIA) has recently been developed for assaying HCV core protein serum. To assess utility measurements serum during course treatment C, we studied 27 who were treated a single schedule interferon alfa (IFN–α) (9 million units per dose 24 weeks; total dose, 720 units). Eleven responded clearance RNA fall...
Epidermal growth factor receptor (EGFR) is overexpressed in various cancers, including non-small cell lung cancer (NSCLC), and some somatic cells at a limited level, rendering it an attractive antitumor target. In this study, we engineered chimeric antigen (CAR)-T using the piggyBac transposon system, autologous artificial antigen-presenting cells, natural ligands of EGFR. We showed that approach yielded CAR-T with favorable phenotypes CAR positivity. They exhibited potent activity against NSCLC both
As the prognosis of relapsed/refractory (R/R) acute myeloid leukaemia (AML) remains poor, novel treatment strategies are urgently needed. Clinical trials have shown that chimeric antigen receptor (CAR)-T cells for AML more challenging than those targeting CD19 in B-cell malignancies. We recently developed piggyBac-modified ligand-based CAR-T target CD116/CD131 complexes, also known as GM-CSF (GMR), juvenile myelomonocytic leukaemia. This study therefore aimed to develop a therapeutic method...
Oxidative stress is one of the major factors in doxorubicin (DOX)-induced cardiomyopathy. Lectin-like oxidized low-density lipoprotein (oxLDL) receptor-1 (LOX-1) plays an important role to regulate cardiac remodeling and oxidative after ischemia-reperfusion. Therefore, we examined whether or not LOX-1 contributes pathogenesis DOX-induced Cardiomyopathy was induced by a single intraperitoneal injection DOX into wild-type (WT) mice knockout (KO) mice. Echocardiography catheter-based...
Chimeric antigen receptor (CAR)-T cell therapy redirected to specific antigens on tumor cells is a promising immunotherapy strategy for various cancers. Most target are also expressed normal tissues at varying levels, and therefore, considerable challenge in the field determining safety profiles, including life-threatening off-tumor off-target toxicities. The granulocyte-macrophage colony-stimulating factor (hGMR) CAR T-cell subset of acute myelocytic leukaemia, although it monocytes,...