A5032685055- Metabolism and Genetic Disorders
- Amino Acid Enzymes and Metabolism
- Folate and B Vitamins Research
- Heart Rate Variability and Autonomic Control
- Ginkgo biloba and Cashew Applications
- Drug Transport and Resistance Mechanisms
- Neonatal Health and Biochemistry
- Neurological Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Atrial Fibrillation Management and Outcomes
- Cardiac electrophysiology and arrhythmias
- Liquid Crystal Research Advancements
- Stress Responses and Cortisol
- Hemoglobinopathies and Related Disorders
- Advanced X-ray and CT Imaging
- Digital Radiography and Breast Imaging
- Bullying, Victimization, and Aggression
- Dialysis and Renal Disease Management
- Central Venous Catheters and Hemodialysis
- Erythrocyte Function and Pathophysiology
- Organ Transplantation Techniques and Outcomes
- Peroxisome Proliferator-Activated Receptors
- Calpain Protease Function and Regulation
- Photonic Crystals and Applications
- Pharmacological Effects and Toxicity Studies
Kanazawa University
2007-2024
Niigata Seiryo University
2024
Health Sciences University of Hokkaido
2006-2023
Children's Hospital of Michigan
2022
Central Michigan University
2022
Municipal Hirakata City Hospital
2022
Kyushu University
2022
Nagoya University Hospital
2019-2020
Kobe University
2019-2020
Tohoku University
2004-2020
Some organic anions are absorbed from the gastrointestinal tract through carrier-mediated transport mechanism(s), which may include proton-coupled transport, anion exchange and others. However, molecular identity of transporters localized at apical membrane human intestinal epithelial cells has not been clearly demonstrated. In present study, we focused on transporting polypeptide OATP-B examined its subcellular localization functionality in small intestine. Localization was determined by...
Carnitine is essential for β-oxidation of fatty acids, and a defect cell membrane transport carnitine leads to fatal systemic deficiency. We have already shown that the organic cation/carnitine transporter OCTN2 primary cause In present study, we further isolated characterized new members OCTN family, OCTN1 -3, in mice. All three were expressed commonly kidney, -2 also various tissues, whereas OCTN3 was by predominant expression testis. When their cDNAs transfected into HEK293 cells, cells...
COVID-19 is spreading worldwide, causing various social problems. The aim of the present study was to verify reliability and validity Japanese version Fear Scale (FCV-19S) ascertain FCV-19S effects on assessment people's coping behavior. After back-translation scale, 450 participants were recruited from a crowdsourcing platform. These responded FCV-19S, versions Hospital Anxiety Depression scale (HADS) Perceived Vulnerability Disease (PVD), which assesses behaviors such as stockpiling health...
OCTN1 (SLC22A4) transports cationic compounds such as tetraethylammonium in a pH-sensitive and sodium-independent manner cultured cells, is expressed wide variety of tissues, including kidney, muscle, placenta, heart, others. This study focused on the clarification its subcellular distribution kidney driving force to throw light pharmacological physiological roles OCTN1. Uptake [14C]tetraethylammonium by membrane vesicles prepared from HEK293 cells stably transfected with human cDNA was...
The organic cation/carnitine transporter OCTN2 is responsible for renal tubular reabsorption of its endogenous substrate, carnitine, although physiological role in small intestine remains controversial. Here we present direct evidence a predominant intestinal absorption carnitine based on experiments with juvenile visceral steatosis (<i>jvs</i>) mice, which have hereditary deficiency the <i>octn2</i> gene. Uptake assessed an Ussing-type chamber system, from apical surface was saturable and...
Pre-harvest sprouting, the germination of mature seeds on mother plant under moist condition, is a serious problem in cereals. To investigate effect reduced abscisic acid (ABA) catabolism hexaploid wheat (Triticum aestivum L.), we cloned ABA 8'-hydroxyase gene which was highly expressed during seed development (TaABA8'OH1) and screened for mutations that lead to catabolism. In screen natural variation, one insertion mutation exon 5 TaABA8'OH1 D genome (TaABA8'OH1-D) identified Japanese...
Spermatogenic immunoglobulin superfamily (SgIGSF) is a mouse protein belonging to the expressed in spermatogenic cells of seminiferous tubules. We produced specific polyclonal antibody against SgIGSF. Western blot analysis testes from postnatal developing mice using this demonstrated multiple immunopositive bands 80–130 kDa, which increased number and size with age. Enzymatic N-glycolysis caused reduction these 70 indicating that SgIGSF glycoprotein its glycosylation pattern extent are...
Sympathetic activation in chronic heart failure (CHF) is greatly augmented at rest but the response to exercise remains controversial. We previously demonstrated that single-unit muscle sympathetic nerve activity (MSNA) provides a more detailed description of physiological stress than multi-unit recordings. The purpose this study was determine whether reflex and discharge properties MSNA are altered during handgrip (HG, 30% maximum voluntary contraction for 3 min) CHF patients (New York...
The Rho/Rho-kinase pathway plays an important role in many cardiovascular diseases such as hypertension, atherosclerosis, heart failure, and myocardial infarction. Although previous studies have shown that Rho-kinase inhibitors reduce ischemia/reperfusion (I/R) injury cytokine production, the of leukocytes during I/R is not well understood. Mice were subjected to 30-min ischemia reperfusion. activity was significantly greater compared sham-operated mice. Administration fasudil, a inhibitor,...
Recently, it was reported that the organic cation/carnitine transporter 1 (OCTN1, <i>SLC22A4</i>) is associated with chronic inflammatory diseases, such as rheumatoid arthritis (RA) and Crohn9s disease. OCTN1 in humans expressed synovial tissues of individuals arthritis. Furthermore octn1 mice inflamed joints collagen-induced arthritis, a model human but not normal mice. should be involved disease present study, regulatory mechanism expression characterized using fibroblast-like synoviocyte...
Atrial fibrillation (AF) is a common complication in heart failure (HF) patients. However, it remains unclear whether irregular ventricular response patterns induced by AF increase sympathetic nerve activity. We measured resting multi- and single-unit muscle activity (MSNA) 21 age-matched HF patients with chronic (n = 11) rhythm or sinus (SR, n 10). The multi-unit MSNA, which was expressed as total activity, similar between + SR the MSNA significantly greater than that (62 ± 9 spikes min(-1)...
Carnitine and acetylcarnitine are important for the acquisition of motility maturation spermatozoa in epididymis. In this study, we examined involvement carnitine/organic cation transporter (OCTN) carnitine transport epididymal mice. Uptake both compounds by was time-dependent partially Na(+)-dependent. Kinetic analyses revealed presence a high-affinity system spermatozoa, with K(m) values 23.6 6.57 muM respectively Na(+). Expression OCTN2 OCTN3 confirmed immunofluorescence analysis. The...
Abstract Patients with end-stage renal disease (ESRD) have a low nutritional status and high mortality risk. The geriatric risk index (GNRI) is predictive marker of malnutrition. However, the association between unplanned hemodialysis (HD) GNRI remains unclear. In total, 162 patients underwent HD at our hospital. They were divided into two groups: those initiation central venous catheter (CVC; n = 62) planned prepared vascular access (n 100). There no significant differences in sex, age,...
Carnitine is extensively accumulated in epididymis. also testis at higher concentration than the plasma and used spite of presence blood-testis barrier. In this study, we examined characteristics carnitine transport primary-cultured rat Sertoli cells, which constitute a part Uptake [3H]carnitine (11.4 nM) from basal side cells was Na+-dependent significantly decreased 10 microM (48.0 +/- 7.4% control) or 100 unlabeled (14.6 5.7% control). Furthermore, uptake inhibited acetyl-L-carnitine,...