A5063611864- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Pharmacological Effects and Toxicity Studies
- Wnt/β-catenin signaling in development and cancer
- Antibiotics Pharmacokinetics and Efficacy
- Cancer-related gene regulation
- Cancer Treatment and Pharmacology
- Adenosine and Purinergic Signaling
- Ion Transport and Channel Regulation
- Neonatal Health and Biochemistry
- Pregnancy and Medication Impact
- Pancreatic and Hepatic Oncology Research
- RNA Interference and Gene Delivery
- Folate and B Vitamins Research
- Chemotherapy-related skin toxicity
- HIV/AIDS drug development and treatment
- Gastroesophageal reflux and treatments
- Pharmacogenetics and Drug Metabolism
- Pregnancy and preeclampsia studies
- Peroxisome Proliferator-Activated Receptors
- Mitochondrial Function and Pathology
- Biochemical effects in animals
- Cancer therapeutics and mechanisms
- Colorectal Cancer Treatments and Studies
Kanazawa University
2014-2024
Kanazawa University Hospital
2015-2024
Second Affiliated Hospital of Zhengzhou University
2024
Keio University
2008-2014
Translational Genomics Research Institute
2013
Harbin Medical University
2013
First Affiliated Hospital of Harbin Medical University
2013
Japanese Red Cross Kanazawa Hospital
2013
Mie Chuo Medical Center
2012
Kyushu Kyoritsu University
2005-2008
Primary carnitine deficiency, because of a defect the tissue plasma membrane transporters, causes critical symptoms. However, transporter has not been molecularly identified. In this study, we screened human kidney cDNA library and assembled cDNA-encoding OCTN2 as homologue organic cation OCTN1, then examined function transporter. OCTN2-cDNA encodes polypeptide 557 amino acids with 75.8% similarity to OCTN1. Northern blot analysis showed that is strongly expressed in kidney, skeletal muscle,...
cDNA for a novel proton/organic cation transporter, OCTN1, was cloned from human fetal liver and its transport activity investigated. OCTN1 encodes 551-amino acid protein with 11 transmembrane domains one nucleotide binding site motif. It is strongly expressed in kidney, trachea, bone marrow several cancer cell lines, but not adult liver. When HEK293 cells, exhibited saturable pH-dependent [3H]tetraethyl ammonium uptake higher at neutral alkaline pH than acidic pH. Furthermore, treatment...
C-C motif chemokine receptor (CCR)2 and its ligand, monocyte chemoattractant protein (MCP)-1, are pivotal for adipose tissue macrophage (ATM) recruitment the development of insulin resistance. However, other systems also may play a role in these processes. In this study, we investigated CCR5 obesity-induced inflammation We analyzed expression levels ligands white (WAT) genetically (ob/ob) high-fat (HF) diet-induced obese (DIO) mice. Furthermore, examined metabolic phenotype Ccr5(-/-) were...
Genetic polymorphisms of human organic anion transporting polypeptides OATP-C (SLC21A6) and OATP-B (SLC21A9) in the Japanese population were analyzed. The allele frequencies <i>OATP-C*1a</i>,<i>OATP-C*1b</i> (N130D), <i>OATP-C*1c</i> (R152K D241N), <i>OATP-C*5</i> (V174A) 35.2, 53.7, 0, 0.7%, respectively, 267 healthy subjects. In the<i>OATP-C</i> gene, we found a novel called<i>OATP-C*15</i> possessing two single nucleotide (SNPs), N130D V174A, simultaneously. frequency of<i>OATP-C*15</i>...
In the present study, functional characteristics of organic cation transporter (OCTN)1, which was cloned as pH-dependent tetraethylammonium (TEA) when expressed in mammalian human embryonic kidney (HEK)293 cells, were further investigated using Xenopus oocytes well HEK293 cells gene expression systems. When OCTN1-derived complementary RNA injected into oocytes, transport [14C]TEA observed same cells. contrast, a replacement sodium ions with potassium surrounding medium did not cause any...
Carnitine is essential for β-oxidation of fatty acids, and a defect cell membrane transport carnitine leads to fatal systemic deficiency. We have already shown that the organic cation/carnitine transporter OCTN2 primary cause In present study, we further isolated characterized new members OCTN family, OCTN1 -3, in mice. All three were expressed commonly kidney, -2 also various tissues, whereas OCTN3 was by predominant expression testis. When their cDNAs transfected into HEK293 cells, cells...
Carnitine deficiency, either primary or drug-induced, causes critical symptoms and is thought to involve alteration of active transport carnitine across the plasma membrane tissues as underlying mechanism. Recently, we showed that human organic cation transporter, hOCTN2, cloned a member transporter family, physiologically important Na(+)-dependent high-affinity in humans. In this study, further characterized functional properties hOCTN2 examined interaction between hOCTN2-mediated...
Abstract Because the significance of P-glycoprotein in in-vivo secretion β-blockers intestinal epithelial cells is unclear, secretory mechanism for and other drugs has been evaluated. Uptake acebutolol, celiprolol, nadolol timolol, antiarrhythmic agent, quinidine by multidrug-resistant leukaemic cell line variant K562/ADM was significantly lower than that drug-sensitive K562 cells, suggesting these are transported out cells. The reduced uptake acebutolol drug-resistant reversed treating with...
Human organic anion transporting polypeptide OATP-B (OATP2B1) is a pH-sensitive transporter expressed in the apical membranes of small intestinal epithelial cells. In this study, we have examined contribution to uptake [<sup>3</sup>H]estrone-3-sulfate Caco-2 cells comparison with those its homologs OATP-D (OATP3A1) and OATP-E (OATP4A1). Immunocytochemical study revealed that The by was Na<sup>+</sup>-independent inhibited several anions. It showed biphasic saturation kinetics...
OCTN2 is an Na<sup>+</sup>-dependent transporter for carnitine, which essential fatty acid metabolism, and its functional defect leads to fatal systemic carnitine deficiency (SCD). It also transports the organic cation tetraethylammonium (TEA) in Na<sup>+</sup>-independent manner. Here, we studied multifunctionality of OCTN2, by examining transport characteristics cells transfected with mouse juvenile visceral steatosis (<i>jvs</i>) mice that exhibit a SCD phenotype owing mutation gene. The...
Transport of l ‐[ 3 H]carnitine and acetyl‐ at the blood–brain barrier (BBB) was examined by using in vivo vitro models. In brain uptake H]carnitine, determined a rat perfusion technique, decreased presence unlabeled ‐carnitine absence sodium ions. Similar transport properties for and/or were observed primary cultured capillary endothelial cells (BCECs) rat, mouse, human, porcine bovine, immortalized BCECs, RBEC1. Uptakes RBEC1 ion‐dependent, saturable with K m values 33.1 ± 11.4 µ 31.3 11.6...
Accumulation of visceral fat induces various symptoms metabolic syndrome such as insulin resistance and abnormal glucose/lipid metabolism eventually leads to the onset ischemic cerebrovascular diseases. Geniposide, which is iridoid glycoside from fruit Gardenia jasminoides ELLIS, recognized being useful against hyperlipidemia fatty liver. In order clarify effect geniposide on disease-based accumulation relevant molecular mechanism, experiments were performed in spontaneously obese Type 2...
The present study examined the influence of cimetidine on nephrotoxicity and antitumor effects cisplatin in vitro vivo. When serum concentration was maintained over 20 µg/ml for 4 h by bolus continuous intravenous infusion, prevented without influencing activity. Cimetidine antioxidant N-acetylcysteine (NAC) significantly inhibited growth inhibition cells originating from kidney, but not SOSN2 osteosarcoma cells. (1 mM) also did platinum cells, regardless whether organic cation transporter 2...
In the liver, signal transducer and activator of transcription 3 (STAT3) plays an important role in suppression gluconeogenic enzyme expression. While obesity-associated endoplasmic reticulum (ER) stress has been shown to increase hepatic expression, ER STAT3-dependent regulation such expression is unclear. The current study aimed elucidate effect on Genetically obese/diabetic db/db mice mouse–derived isolated hepatocytes were used as models. A tyrosine phosphatase inhibitor, a deacetylation...
Approximately half of autism spectrum disorder (ASD) individual suffer from comorbid intellectual disabilities (ID). Oxytocin (OXT) receptors are highly expressed in temporal lobe structures and likely to play a modulatory role excitatory/inhibitory balance, at least based on animal model findings. Thus, it is feasible that the representative group Kanner type ASD subjects OXT could have beneficial effect social communication interaction. The aim this pilot study was investigate feasibility...
The participation of the monocarboxylic acid transporter MCT1 in intestinal absorption weak organic acids has been clarified by functional characterization, use stably transfected cells, and immunohistochemical location tissues. Immunohistochemical analysis anti-MCT1 antibody showed that is distributed throughout upper lower intestines, especially basolateral membrane and, to a lesser extent, brush-border membrane. When gene rat was into MDA-MB231 transport benzoic acid, model generally...