A5022669876- Cardiac Imaging and Diagnostics
- Cardiovascular Function and Risk Factors
- Cardiac Ischemia and Reperfusion
- Cardiovascular Disease and Adiposity
- Cardiac Fibrosis and Remodeling
- Cardiac electrophysiology and arrhythmias
- Coronary Interventions and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Autophagy in Disease and Therapy
- Cardiovascular Health and Disease Prevention
- Signaling Pathways in Disease
- Atrial Fibrillation Management and Outcomes
- Tissue Engineering and Regenerative Medicine
- Heart Rate Variability and Autonomic Control
- Mesenchymal stem cell research
- Cell death mechanisms and regulation
- Cardiac Arrest and Resuscitation
- Blood Pressure and Hypertension Studies
- Hormonal Regulation and Hypertension
- Cardiomyopathy and Myosin Studies
- Cardiac Arrhythmias and Treatments
- Anesthesia and Neurotoxicity Research
- Angiogenesis and VEGF in Cancer
- Acute Myocardial Infarction Research
- Nitric Oxide and Endothelin Effects
Gifu University
2016-2025
Gifu Municipal Hospital
1999-2025
Tohoku University
2018-2022
Nippon Shinyaku (Japan)
1998-2019
Gifu University Hospital
2011-2018
Tokyo Metropolitan University
2018
Mansoura University
2018
Matsunami General Hospital
1999-2016
Gifu Prefectural General Medical Center
2012-2016
Beth Israel Deaconess Medical Center
2016
Little is known about the association between autophagy and diabetic cardiomyopathy. Also unknown are possible distinguishing features of cardiac in type 1 2 diabetes. In hearts from streptozotocin-induced mice, diastolic function was impaired, though autophagic activity significantly increased, as evidenced by increases microtubule-associated protein light chain 3/LC3 LC3-II/-I ratios, SQSTM1/p62 (sequestosome 1) CTSD (cathepsin D), abundance vacuoles lysosomes detected...
Background — The purpose of the present study was to define whether integrated backscatter (IB) combined with conventional intravascular ultrasound (IVUS) makes tissue characterization coronary arterial plaques possible. Methods and Results IB-IVUS performed in arteries (total 18 segments) 9 patients at autopsy, findings were compared histology. RF signals, which digitized 2 GHz 8-bit resolution, obtained an IVUS system a 40-MHz catheter. IB values signal from region interest (ROI) (100-μm...
Background —The presence of apoptotic myocytes has been reported in human hearts with dilated cardiomyopathy (DCM) on the basis a positive finding DNA situ nick end-labeling (TUNEL). However, ultrastructural evidence myocyte apoptosis not obtained. Methods and Results —A total 80 endomyocardial biopsies were obtained from right left ventricles 20 patients DCM normal control subjects. TUNEL-positive found by light microscope 15% specimens (controls, 0%, P <0.05), percentage per section was...
Background —Modes of cell death have been defined morphologically as apoptosis and oncosis. Infarcted myocytes reported to show apoptosis, revealed by DNA fragmentation ladder in situ terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling (TUNEL) at the light microscopic level. We investigated whether TUNEL-positive infarcted apoptotic or oncotic ultrastructures using electron TUNEL, which can simultaneously observe ultrastructure same myocytes. Methods Results —Thirty rabbits...
We investigated whether the improvement of cardiac function and remodeling after myocardial infarction (MI) by granulocyte colony-stimulating factor (G-CSF) relates to acceleration healing process, in addition regeneration.In a 30-minute coronary occlusion reperfusion rabbit model, saline (S) or 10 microg x kg(-1) d(-1) human recombinant G-CSF (G) was injected subcutaneously from 1 5 days MI. Smaller left ventricular (LV) dimension, increased LV ejection fraction, thicker infarct-LV wall...
Abstract —Myocardial infarction (MI) progresses from the acute death of myocytes and infiltration inflammatory cells into granulation, followed by scars. During healing process, myocardial interstitial cell population in infarcted tissues increases markedly then decreases. We postulated that apoptosis is responsible for this process. Twenty-four male Japanese white rabbits underwent a 30-minute occlusion left coronary artery reperfusion 2 days, weeks, or 4 weeks (n=8 each). The histological...
Doxorubicin is a highly effective antineoplastic drug, but its clinical use limited by adverse side effects on the heart. We investigated possible protective of erythropoietin against doxorubicin-induced cardiomyopathy.Cardiomyopathy was induced in mice single intraperitoneal injection doxorubicin (15 mg/kg). In some cases, human recombinant (5000 U/kg) started simultaneously. Two weeks later, left ventricular dilatation and dysfunction were apparent given significantly attenuated treatment....
Ischemia is known to potently stimulate autophagy in the heart, which may contribute cardiomyocyte survival. In vitro, transfection with small interfering RNAs targeting Atg5 or Lamp-2 (an autophagy-related gene necessary, respectively, for initiation and digestion step of autophagy), specifically inhibited autophagy, diminished survival among cultured cardiomyocytes subjected anoxia significantly reduced their ATP content, confirming an autophagy-mediated protective effect against anoxia....
Autophagy is activated in cardiomyocytes ischaemic heart disease, but its dynamics and functional roles remain unclear after myocardial infarction. We observed the of cardiomyocyte autophagy examined role during postinfarction cardiac remodelling. Myocardial infarction was induced mice by ligating left coronary artery. During both subacute chronic stages (1 3 weeks postinfarction, respectively), found to be surviving cardiomyocytes, as demonstrated up-regulated expression...
Fibrosis and progressive failure are prominent pathophysiological features of hearts after myocardial infarction (MI). We examined the effects inhibiting transforming growth factor-beta (TGF-beta) signaling on post-MI cardiac fibrosis ventricular remodeling function.MI was induced in mice by left coronary artery ligation. An adenovirus harboring soluble TGF-beta type II receptor (Ad.CAG-sTbetaRII), a competitive inhibitor TGF-beta, then injected into hindlimb muscles day 3 MI (control,...
Active autophagy has recently been reported in doxorubicin-induced cardiotoxicity; here we investigated its pathophysiological role.Acute cardiotoxicity was induced green fluorescent protein-microtubule-associated protein 1 light chain 3 (GFP-LC3) transgenic mice by administering two intraperitoneal injections of 10 mg/kg doxorubicin with a day interval. A starvation group deprived food for 48 h before each injection to induce advance. Doxorubicin treatment caused left ventricular dilatation...
Rationale: Multilineage-differentiating stress enduring (Muse) cells, pluripotent marker stage-specific embryonic antigen-3 + are nontumorigenic endogenous pluripotent-like stem cells obtainable from various tissues including the bone marrow. Their therapeutic efficiency has not been validated in acute myocardial infarction. Objective: The main objective of this study is to clarify intravenously infused rabbit autograft, allograft, and xenograft (human) marrow-Muse a infarction model their...
We previously reported that gallic acid (3,4,5-trihydroxybenzoic acid), a naturally occurring plant phenol, can induce apoptosis in four kinds of human lung cancer cell lines vitro. The present study further investigated the vivo anti-tumor effects orally administered acid. Gallic reduced viability LL-2 mouse cells vitro dose dependently, with 50% inhibitory concentration (IC50) value around 200 microM. C57Black mice were transplanted cells, and (1 mg/ml drinking water, ad libitum) and/or...
Background —It has been thought that the thrombi and bleeding in plaques occur after plaque rupture or endothelial damage from vessels with mild stenosis suddenly occlude lumen cause acute myocardial infarction (AMI). However, our hypothesis is may not lumen. Methods Results —The study group consisted of 20 patients who had coronary angiograms performed within 1 week (3±3 days) before AMI control 6 to 18 months (282±49 AMI. The features infarct-related segments (IRCS) at 3 days were presence...
Background —The process of progression in coronary artery disease is unknown. Methods and Results subjects were 36 patients with objective vessels clinically significant (≥15% per year) whom 4 serial arteriograms (CAGs) performed at intervals ≈4 months a 1-year period. The degree percent stenosis between each 2 CAGs was classified as marked (M: ≥15%), slight (S: 5% to 14%), no (N: <5%). From the pattern progression, 14 type 1 (N→N→M 13 S→S→M vessel) 22 without (S→S→S 18 vessels, N→S→S 4)....
Background The purpose of this study was to determine whether treatment with granulocyte colony-stimulating factor (G-CSF), which mobilizes endothelial progenitor cells from bone marrow, can safely improve the clinical outcomes patients atherosclerotic peripheral artery disease (PAD). Methods and Results Thirty-nine intractable PAD were randomly assigned 3 groups: a negative control group (n=12) treated conventional drug therapy; positive (n=13) therapy plus marrow transplantation (BMT);...
Late treatment with erythropoietin (EPO), as well the administration before onset of or during acute stage myocardial infarction (MI), has recently been shown to mitigate post-MI heart failure. We investigated mechanisms, including downstream signaling pathways, for beneficial effect late EPO on chronic failure.EPO (1500 U/kg, twice a week) was administered mice beginning 6 weeks after induction large MI. The 4 diminished left ventricular dilatation and improved function. It significantly...