A5008895763- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Pancreatic and Hepatic Oncology Research
- Groundwater flow and contamination studies
- Monoclonal and Polyclonal Antibodies Research
- Fungal and yeast genetics research
- Biochemical and Structural Characterization
- Neuroendocrine Tumor Research Advances
- Gastric Cancer Management and Outcomes
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Soil and Unsaturated Flow
- Immunotherapy and Immune Responses
- Gallbladder and Bile Duct Disorders
- Gastrointestinal Tumor Research and Treatment
- vaccines and immunoinformatics approaches
- Transgenic Plants and Applications
- Esophageal Cancer Research and Treatment
- Biotin and Related Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Peptidase Inhibition and Analysis
- Liver Disease Diagnosis and Treatment
- Esophageal and GI Pathology
Kobe University
2016-2025
Aichi Cancer Center
2016-2025
Osaka Research Institute of Industrial Science and Technology
2025
Tottori University
2015-2022
National Institute of Technology
2020
Fukushima College
2020
Tsuchiura Kyodo General Hospital
2018
Nagoya Central Hospital
2018
Graduate School USA
2016-2017
Sapporo Medical University
2014-2015
Intraductal papillary mucinous neoplasms (IPMNs) are precursor lesions of pancreatic adenocarcinoma. Artificial intelligence (AI) is a mathematical concept whose implementation automates learning and recognizing data patterns. The aim this study was to investigate whether AI via deep algorithms using endoscopic ultrasonography (EUS) images IPMNs could predict malignancy.This retrospective involved the analysis patients who underwent EUS before pancreatectomy had pathologically confirmed in...
ObjectiveThe combination chemotherapy consisting of cisplatin and etoposide, one the standard regimens for small cell lung cancer, has been widely used to treat extrapulmonary poorly differentiated neuroendocrine carcinomas. However, there were no prior reports limited hepatobiliary tract pancreas as primary sites.
Abstract Background The filamentous fungus T. reesei effectively degrades cellulose and is known to produce various cellulolytic enzymes such as β-glucosidase, endoglucanase, cellobiohydrolase. expression levels of each cellulase are controlled simultaneously, their ratios synergetic effects important for effective degradation. However, in recombinant Saccharomyces cerevisiae , it difficult simultaneously control many different enzymes. To construct engineered yeast with efficient...
The use of enzymes is a promising approach for site-specific protein modification on living cells owing to their substrate specificity. Herein we describe general strategy the cell surface proteins with synthetic molecules by using Sortase, transpeptidase from Staphylococcus aureus. short peptide tag LPETGG genetically introduced C terminus target protein, expressed surface. Subsequent addition Sortase and an N-terminal triglycine-containing probe results in labeling tagged protein. We were...
A prospective clinical study was conducted to evaluate the safety, feasibility, and efficacy of endoscopic ultrasound (EUS)-guided choledochoduodenostomy (CDS) with direct metallic stent placement using a prototype forward-viewing echoendoscope. The indication for EUS – CDS in this lower biliary obstruction only, not failed drainage, because aim first-line drainage therapy. technical functional success rates were 94 % (17 /18) (16 /17), respectively. Early complications (focal peritonitis)...
Abstract Background Hydrolysis of cellulose requires the action cellulolytic enzymes endoglucanase, cellobiohydrolase and β-glucosidase. The expression ratios synergetic effects these significantly influence extent specific rate degradation. In this study, using our previously developed method to optimize cellulase-expression levels in yeast, we constructed a diploid Saccharomyces cerevisiae strain optimized for enzymes, attempted improve cellulose-degradation activity enable direct ethanol...
Glucose and xylose are the major components of lignocellulose. Effective utilization both sugars can improve efficiency bioproduction. Here, we report a method termed parallel metabolic pathway engineering (PMPE) for producing shikimate derivatives from glucose-xylose co-substrate. In this method, seek to use glucose mainly target chemical production, supplying essential metabolites cell growth. Glycolysis pentose phosphate completely separated tricarboxylic acid (TCA) cycle. To recover...
Chenopodium quinoa Willd. (quinoa) originated from the Andean region of South America, and is a pseudocereal crop Amaranthaceae family. Quinoa emerging as an important with potential to contribute food security worldwide considered be optimal source for astronauts, due its outstanding nutritional profile ability tolerate stressful environments. Furthermore, plant pathologists use representative diagnostic host identify virus species. However, molecular analysis limited by genetic...
Biological applications of nanoparticles are rapidly increasing, which introduces new possibilities to improve the efficacy radiotherapy. Here, we synthesized titanium peroxide (TiOxNPs) and investigated their as novel agents that can potently enhance effects radiation in treatment pancreatic cancer. TiOxNPs polyacrylic acid-modified (PAA-TiOxNPs) were from anatase-type dioxide (TiO2NPs). The size morphology PAA-TiOxNPs was evaluated using transmission electron microscopy dynamic light...
Abstract Background There are several types of pancreatic mass, so it is important to distinguish between them before treatment. Artificial intelligence (AI) a mathematical technique that automates learning and recognition data patterns. This study aimed investigate the efficacy our AI model using endoscopic ultrasonography (EUS) images multiple mass (pancreatic ductal adenocarcinoma [PDAC], adenosquamous carcinoma [PASC], acinar cell [ACC], metastatic tumor [MPT], neuroendocrine [NEC],...
This study aimed to address the limitations of conventional methods for measuring skeletal muscle mass sarcopenia diagnosis by introducing an artificial intelligence (AI) system direct computed tomography (CT) analysis. The primary focus was on enhancing simplicity, reproducibility, and convenience, assessing accuracy speed AI compared with methods. A cohort 3096 cases undergoing CT imaging up third lumbar (L3) level between 2011 2021 were included. Random division into preprocessing cohorts...
ABSTRACT The modularization of biosynthetic pathways is a promising approach for enhancing microbial chemical production. We have developed co-utilization method with glucose and xylose substrates to divide metabolic into distinct production energy modules enhance the biosynthesis para -aminobenzoic acid (pABA) in Escherichia coli . Optimizing initial glucose/xylose concentrations eliminating carbon leakage resulted pABA titer 8.22 g/L (yield: 0.23 g/g glucose). This strategy was then...
Background —It has been thought that the thrombi and bleeding in plaques occur after plaque rupture or endothelial damage from vessels with mild stenosis suddenly occlude lumen cause acute myocardial infarction (AMI). However, our hypothesis is may not lumen. Methods Results —The study group consisted of 20 patients who had coronary angiograms performed within 1 week (3±3 days) before AMI control 6 to 18 months (282±49 AMI. The features infarct-related segments (IRCS) at 3 days were presence...
Background and aims Endoscopic mucosal resection (EMR) is the standard procedure for treatment of colorectal tumors. submucosal dissection (ESD) can be performed larger tumors, but has not been studied in colorectum because technical difficulties complications. We compared outcomes complications after tumors than 20 mm diameter by ESD EMR. Materials methods retrospectively 104 treated with EMR from 1995 to 2004, 85 2005 2009. tumor location, shape, size, time, en bloc rate, recurrence...
In order to achieve direct and efficient fermentation of optically pure D-lactic acid from raw corn starch, we constructed L-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum introduced a plasmid encoding Streptococcus bovis 148 alpha-amylase (AmyA). The resulting strain produced only glucose successfully expressed amyA. With the aid secreting AmyA, starch was accomplished. After 48 h fermentation, 73.2 g/liter lactic with high yield (0.85 g per consumed sugar) an optical...
To allow the comprehensive assessments of yeast expression systems, a simple and immediate method for simultaneously evaluating level plasmid maintenance in was demonstrated. This uses green fluorescent protein (GFP) flow cytometry (FCM) is characterized by dual analysis average intensity GFP fluorescence population GFP-expressing cells. The FCM rapidly quantifies without complex manipulations, such as enzymatic reaction lacZ reporter assay. Moreover, single-cell revealed that proportion...
ABSTRACT The production of optically pure d -lactic acid via xylose fermentation was achieved by using a Lactobacillus plantarum NCIMB 8826 strain whose l -lactate dehydrogenase gene deficient and phosphoketolase genes were replaced with heterologous transketolase gene. After 60 h fermentation, 41.2 g/liter produced from 50 xylose.