A5011631068- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Subcritical and Supercritical Water Processes
- Microtubule and mitosis dynamics
- Diatoms and Algae Research
- Photosynthetic Processes and Mechanisms
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Spectroscopy and Quantum Chemical Studies
- Catalytic Processes in Materials Science
- Micro and Nano Robotics
- Bacteriophages and microbial interactions
- Molecular Junctions and Nanostructures
- Enzyme Structure and Function
- Supramolecular Self-Assembly in Materials
- Nanoparticle-Based Drug Delivery
- Protein Structure and Dynamics
- Advanced Cellulose Research Studies
- Lignin and Wood Chemistry
- RNA and protein synthesis mechanisms
- Molecular spectroscopy and chirality
- DNA and Nucleic Acid Chemistry
- Viral Infectious Diseases and Gene Expression in Insects
Tohoku University
2016-2025
RIKEN Center for Advanced Intelligence Project
2018-2025
Sendai University
2015-2018
Advanced Institute of Materials Science
2010-2016
Graduate School USA
2008-2016
Institute for Materials Research, Tohoku University
2014-2015
Tokyo Metropolitan Komaba High School
2011
Canon (Japan)
2010
Kyoto University
2009
Nagoya University
2009
Control of the morphology ceria nanocrystals is achieved through a simple, rapid, and green chemistry approach by tuning reaction organic ligand molecules with specific crystallographic planes fluorite cubic crystal during supercritical hydrothermal synthesis, as shown in figure. It provides novel self-assembly for producing metal oxide uniform size well-defined shape. Supporting information this article available on WWW under http://www.wiley-vch.de/contents/jc_2089/2007/c0964_s.pdf or from...
A peptide with an affinity for ZnO, selected by a phage-display system, preferentially immobilizes ZnO particles on gold-coated polypropylene plate and assists in the homogeneous assembly of 10 nm diameter nanoparticles into unique flower-like morphologies (see Figure). The is selective binding but not ZnS or Eu2O3. This combinatorial library approach may yield new peptides used to create structures via biomineralization.
Molecular evolution based on mutagenesis is widely used in protein engineering. However, optimal proteins are often difficult to obtain due a large sequence space. Here, we propose novel approach that combines molecular with machine learning. In this approach, conduct two rounds of where an initial library variants train machine-learning model guide for the second-round library. This enables us prepare small suited screening experiments high enrichment functional proteins. We demonstrated...
Biological applications of nanoparticles are rapidly increasing, which introduces new possibilities to improve the efficacy radiotherapy. Here, we synthesized titanium peroxide (TiOxNPs) and investigated their as novel agents that can potently enhance effects radiation in treatment pancreatic cancer. TiOxNPs polyacrylic acid-modified (PAA-TiOxNPs) were from anatase-type dioxide (TiO2NPs). The size morphology PAA-TiOxNPs was evaluated using transmission electron microscopy dynamic light...
The gradual removal of the denaturing reagent guanidine HCl (GdnHCl) using stepwise dialysis with introduction an oxidizing and l-arginine resulted in highly efficient refolding various denatured single-chain Fv fragments (scFvs) from inclusion bodies expressed in<i>Escherichia coli.</i> In this study, influence additives on intermediates scFv was carefully analyzed basis dialysis, it revealed that additive effect critically changes pathway refolding. Circular dichroism tryptophan...
ABSTRACT Supercritical water liquefaction and gasification is reviewed with the introduction of some recent findings by authors. suitable for recovery energy from wet biomass while supercritical opens door to effective treatment species in terms material recovery. Cellulose, one main components biomass, completely dissolved water. Once dissolved, reaction cellulose can take place swiftly hydrolysis pyrolysis. The reaction, otherwise slower than pyrolysis due mass transfer limitation, faster...
Machine learning (ML) is becoming an attractive tool in mutagenesis-based protein engineering because of its ability to design a variant library containing proteins with desired function. However, it remains unclear how ML guides directed evolution sequence space depending on the composition training data. Here, we present ML-guided study enzyme investigate effects known "highly positive" (i.e., have high activity) We performed two separate series Sortase A and without highly positive called...
Abstract Since the early 1990s, Adschiri and his colleagues have performed research on use of supercritical water in diverse applications, including heavy oil reforming, waste polymer decomposition chemical raw materials recovery, cellulose hydrolysis sugar lignin nanoparticle synthesis. Regarding inorganic synthesis, they invented hydrothermal synthesis for continuous flow metal oxide nanoparticles, which has already found worldwide industrial applications. They proposed a two-fluid mixing...
To investigate the role of Vernier zone residues, which are comprised in framework regions and underlie complementarity-determining (CDRs) antibodies, specific, high affinity interactions antibodies with their targets, we focused on variable domain fragment murine anti-human epidermal growth factor receptor antibody 528 (m528Fv). Grafting CDRs m528Fv onto a selected region human referred to as humanization, reduced antibody's for its target by 1/40. The reduction was due substantial negative...
We report magnetism of spinel ${\text{Co}}_{3}{\text{O}}_{4}$ nanocrystals with average diameters $\ensuremath{\sim}25$ and $\ensuremath{\sim}100\text{ }\text{nm}$ under magnetic fields up to 50 kOe. The nanoparticles were controlled be cubic in shape. In the smaller nanocrystal sample $(d\ensuremath{\sim}25\text{ }\text{nm})$, a spontaneous moment increased steeply below N\'eel point, ${T}_{N}=32\text{ }\text{K}$ bulk sample, whereas similar samples larger crystal...
Abstract We synthesized a “cyborg protein,” wherein synthetic molecule partially substitutes the main peptide chain by linking 2 protein domains with oligomer. Green fluorescent (GFP) served as model for constructing cyborg proteins. prepared circularly permuted GFP (cpGFP) new termini between β10 and β11, where original N- C-termini were linked cleavable loop. The was constructed from cpGFP β11 oligo(ethylene glycol) (OEG) using maleimide–cysteine couplings, followed enzymatic cleavage of...
To study how oligomerization may contribute to the thermostability of archaeon proteins, we focused on a hexameric protein, protein L-isoaspartyl-O-methyltransferase from Sulfolobus tokodaii (StoPIMT). The crystal structure shows that StoPIMT has distinctive composed monomers consisting two domains: an S-adenosylmethionine-dependent methyltransferase fold domain and C-terminal alpha-helical domain. includes three interfacial contact regions: major, minor, coiled-coil. Several deletion...
Size-controllable TiO2 nanosheets with highly exposed {001} facets were synthesized by a hydrothermal method. The particle sizes ranged from 25 nm to submicrometres carefully adjusting the F/Ti molar ratio. smaller than 100 have higher photocatalytic activity and are stable in degradation of organic dyes.