A5067662708- Seed and Plant Biochemistry
- Advanced Condensed Matter Physics
- Genetic and Environmental Crop Studies
- Multiferroics and related materials
- Magnetic and transport properties of perovskites and related materials
- Physics of Superconductivity and Magnetism
- Chromosomal and Genetic Variations
- Food composition and properties
- Advances in Cucurbitaceae Research
- Nuclear materials and radiation effects
- Iron-based superconductors research
- Plant Reproductive Biology
- Magnetic properties of thin films
- Genetic Mapping and Diversity in Plants and Animals
- Rare-earth and actinide compounds
- Plant Molecular Biology Research
- Surface and Thin Film Phenomena
- Genetic diversity and population structure
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- High-pressure geophysics and materials
- Quantum and electron transport phenomena
- Wheat and Barley Genetics and Pathology
Kyoto University
2014-2024
Ōtani University
2024
Kyoto College of Medical Science
2021
Meiji University
2013-2020
University of Toyama
2011-2013
Nagoya University
1998-2012
Chuo University
2007-2009
Osaka University Hospital
2007
Japan Science and Technology Agency
1998-2001
Mie University
1991-1992
Buckwheat (Fagopyrum esculentum Moench; 2n = 2x 16) is a nutritionally dense annual crop widely grown in temperate zones. To accelerate molecular breeding programmes of this important crop, we generated draft assembly the buckwheat genome using short reads obtained by next-generation sequencing (NGS), and constructed Genome DataBase. After assembling reads, determined 387,594 scaffolds as sequence (FES_r1.0). The total length FES_r1.0 was 1,177,687,305 bp, N50 25,109 bp. Gene prediction...
Chenopodium quinoa Willd. (quinoa) originated from the Andean region of South America, and is a pseudocereal crop Amaranthaceae family. Quinoa emerging as an important with potential to contribute food security worldwide considered be optimal source for astronauts, due its outstanding nutritional profile ability tolerate stressful environments. Furthermore, plant pathologists use representative diagnostic host identify virus species. However, molecular analysis limited by genetic...
The different forms of flowers in a species have attracted the attention many evolutionary biologists, including Charles Darwin. In Fagopyrum esculentum (common buckwheat), occurrence dimorphic flowers, namely short-styled and long-styled is associated with type self-incompatibility (SI) called heteromorphic SI. floral morphology intra-morph incompatibility are both determined by single genetic locus named S-locus. Plants heterozygous (S/s) plants homozygous recessive (s/s) at Despite recent...
Abstract Drought severely damages crop production, even under conditions so mild that the leaves show no signs of wilting. However, it is unclear how field-grown plants respond to drought. Here, we through six years field trials ridges are a useful experimental tool mimic drought stress in field. Mild reduces inorganic phosphate levels activate starvation response (PSR) soybean Using Arabidopsis thaliana and its mutant grown pots controlled environments, demonstrate PSR occurs before...
Dihydroflavonol 4-reductase (DFR) is the key enzyme committed to anthocyanin and proanthocyanidin biosynthesis in flavonoid biosynthetic pathway. DFR proteins can catalyse mainly three substrates (dihydrokaempferol, dihydroquercetin, dihydromyricetin), show different substrate preferences. Although relationships between preference amino acids region responsible for specificity have been investigated several plant species, molecular basis of not yet fully understood.By using degenerate...
We report low-temperature thermal conductivity $\kappa$ of pyrochlore Yb$_2$Ti$_2$O$_7$, which contains frustrated spin-ice correlations with significant quantum fluctuations. In the disordered spin-liquid regime, $\kappa(H)$ exhibits a nonmonotonic magnetic field dependence, is well explained by strong spin-phonon scattering and monopole excitations. show that excitation energy monopoles strongly suppressed from dispersionless classical monopoles. Moreover, in stark contrast to diffusive...
To evaluate the potential of genomic selection (GS), a experiment with GS and phenotypic (PS) was performed in an allogamous crop, common buckwheat (Fagopyrum esculentum Moench). indirectly select for seed yield per unit area, which cannot be measured on single-plant basis, index constructed from seven agro-morphological traits measurable single plant basis. Over 3 years, we two one PS cycles year improvement index. In GS, prediction model updated every basis genotypes 14,598–50,000 markers...
We investigated the phylogenetic and biogeographic relationships of natural populations diploid tetraploid Fagopyrum cymosum (Polygonaceae). Intraspecific variation chloroplast DNA sequences was detected in three regions approximately 5 kb long total: 3' end rbcL, accD associated intergenic spacer region, trnC (GCA)-rpoB trnK (UUU) intron, matK region. The accessions F. were divided into two major groups, a Tibet-Himalayan clade Yunnan-Sichuan clade, with high bootstrap probability. It...
We present a study of the magnetoresistance and Hall effect in narrow-gap semiconductor FeSb2 at low temperatures. Both electrical resistivities show unusual magnetic field dependence low-temperature range where large Seebeck coefficient was observed. By applying two-carrier model, we find that carrier concentration decreases from 1 down to 10^-4 ppm/unit cell mobility increases 2000 28000 cm2/Vs with decreasing temperature 30 4 K. At lower temperatures, magnetoresistive behavior drastically...
The very nature of the ground state pyrochlore compound ${\mathrm{Yb}}_{2}{\mathrm{Ti}}_{2}{\mathrm{O}}_{7}$ is much debated, because experimental results demonstrate evidence for either a disordered or long-range ordered state. Indeed, delicate balance exchange interactions and anisotropy believed to lead competing states, such as quantum spin liquid ferromagnetic which may originate from an Anderson-Higgs transition. We present detailed magnetization study demonstrating first-order...
For genetic studies and genomics-assisted breeding, particularly of minor crops, a genotyping system that does not require priori genomic information is preferable. Here, we demonstrated the potential novel array-based for rapid construction high-density linkage map quantitative trait loci (QTL) mapping. By using system, successfully constructed an accurate, common buckwheat (Fagopyrum esculentum Moench); was composed 756 included 8,884 markers. The number groups converged to eight, which...
Abstract How do separate sexes originate and evolve? Plants provide many opportunities to address this question as they have diverse mating systems (dioecy) that evolved times independently. The classic “two-factor” model for evolution of proposes males females can evolve from hermaphrodites via the spread male female sterility mutations turn into males, respectively. This widely accepted was inspired by early genetic work in dioecious white campion (Silene latifolia) revealed presence two...
Next-generation sequencing (NGS) library construction often involves using restriction enzymes to decrease genome complexity, enabling versatile polymorphism detection in plants. However, plant leaves frequently contain impurities, such as polyphenols, necessitating DNA purification before enzymatic reactions. To overcome this problem, we developed a PCR-based method for expeditious NGS preparation, offering flexibility number of detected polymorphisms. By substituting segment the simple...
DNA sequences of the rbcL and accD 5’ coding regions their intergenic region (IGR) were determined in 20 taxa 12 species Fagopyrum . By comparing sequence data constructing phylogenetic trees, relationships among investigated. The IGR have evolved about five times faster than region; hence trees based on region, IGR, these combined, had higher resolution alone. classification was almost perfectly consistent with by Ohnishi Matsuoka (1996, Genes & Genetic Systems 71: 383–390). New facts...
Abstract Halophytes are plants that grow in high-salt environments and form characteristic epidermal bladder cells (EBCs) important for saline tolerance. To date, however, little has been revealed about the formation of these structures. determine genetic basis their formation, we applied ethylmethanesulfonate mutagenesis obtained two mutants with reduced levels EBCs ( rebc ) abnormal chloroplasts. In silico subtraction experiments phenotype was caused by mutation REBC , which encodes a WD40...
The DNA sequences of the internal transcribed spacer (ITS) region rRNA gene were determined in 20 taxa 12 species Fagopyrum. By comparing sequence data and constructing phylogenetic trees, relationships among Fagopyrum established. ITS (ITS1 ITS2) about 3-7 times more variable than those 5.8S subunit gene. classification based on almost coincides with morphology, allozyme variability, nucleotide rbcL-accD chloroplast DNA. A close relationship F. lineare statice-F. leptopodum clade was...
Common buckwheat (Fagopyrum esculentum) (2n = 2x 16) and Fagopyrum homotropicum were mated in an interspecific cross amplified fragment length polymorphism (AFLP) linkage maps constructed by analyzing segregation the F2 population. Six hundred sixty-nine bands identified using 20 AFLP primer combinations, of which 462 (69%) segregated The map F. esculentum has eight groups with 223 markers covering a total 508.3 cM. 211 548.9 There was one to correspondence groups. Three morphological...
The genetic relationships among seven cultivated populations and eight natural of wild common buckwheat were analyzed using amplified fragment length polymorphism (AFLP). distance was estimated for each pair the 15 based on AFLP data, a phylogenetic tree constructed neighbor-joining (NJ) method distance. All grouped in cluster. into two clusters composed (1) Sanjiang group (three from eastern Tibet one population Adong village Yunnan province) (2) province Sichuan province. is more closely...
Buckwheat (Fagopyrum esculentum Moench.) is an annual crop that originated in southern China. The nutritious seeds are used cooking much like cereal grains. outcrossing species with heteromorphic self-incompatibility due to its dimorphic (i.e., short- and long-styled) flowers intra-morph infertility. floral morphology incompatibility both determined by a single S locus. Plants short-styled heterozygous (S/s) plants long-styled homozygous recessive (s/s) at this locus, the S/S genotype not...
Abstract Cultivation of quinoa (Chenopodium quinoa), an annual pseudocereal crop that originated in the Andes, is spreading globally. Because highly nutritious and resistant to multiple abiotic stresses, it emerging as a valuable provide food nutrition security worldwide. However, molecular analyses have been hindered by genetic heterogeneity resulting from partial outcrossing. In this study, we generated 136 inbred lines basis for identification characterization gene functions through...
Common buckwheat (Fagopyrum esculentum Moench 2n = 2x 16) is an outcrossing crop with heteromorphic self-incompatibility due to its distylous flowers, called pin and thrum. In plants, a long style combined short stamens small pollen grains; in thrum large grains. Both the intra-morph flower morphology are controlled by single genetic locus named S locus; plants heterozygous (Ss) homozygous recessive (ss) at this locus. Self-incompatibility obstacle for establishing pure lines fixation of...
MIG-seq (Multiplexed inter-simple sequence repeats genotyping by sequencing) has been developed as a low cost technology, although the number of polymorphisms obtained is assumed to be minimal, resulting in application this technique analyses agricultural plants. We applied 12 plant species that include various crops and investigated relationship between genome size bases can stably sequenced. The loci, which sequenced MIG-seq, are positively correlated. This due linkage simple (SSRs)...