A5075865192- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
- Advanced Nanomaterials in Catalysis
- Biochemical effects in animals
- Biochemical and biochemical processes
- Synthesis and Biological Evaluation
- Fluid Dynamics and Mixing
- Garlic and Onion Studies
- Crustacean biology and ecology
- Fluid Dynamics and Thin Films
- Sulfur-Based Synthesis Techniques
- Synthesis and Reactivity of Heterocycles
- Radical Photochemical Reactions
- Head and Neck Cancer Studies
- Cholesterol and Lipid Metabolism
- Catalytic C–H Functionalization Methods
- Synthesis and Reactions of Organic Compounds
- Cancer, Lipids, and Metabolism
- Aquaculture Nutrition and Growth
- Fungal Plant Pathogen Control
- Radiation Therapy and Dosimetry
- 3D Printing in Biomedical Research
- Nephrotoxicity and Medicinal Plants
Kobe University
1993-2025
Nagoya City University
2013
Wakunaga (Japan)
2001
Saga University
1997
Kyushu University
1997
2-Phenylethanol, known for its rose-like odor and antibacterial activity, is synthesized via exogenous phenylpyruvate by the sequential reaction of decarboxylase (PDC) aldehyde reductase. We first targeted ARO10, a gene from Saccharomyces cerevisiae, identified suitable reductase gene. Co-expression ARO10 yahK in E. coli transformants yielded 1.1 g/L 2-phenylethanol batch culture. hypothesized that there might be bottleneck PDC activity. The computer-based enzyme evolution was utilized to...
ABSTRACT The modularization of biosynthetic pathways is a promising approach for enhancing microbial chemical production. We have developed co-utilization method with glucose and xylose substrates to divide metabolic into distinct production energy modules enhance the biosynthesis para -aminobenzoic acid (pABA) in Escherichia coli . Optimizing initial glucose/xylose concentrations eliminating carbon leakage resulted pABA titer 8.22 g/L (yield: 0.23 g/g glucose). This strategy was then...
In previous studies, titanium peroxide nanoparticles (PAA-TiOx NPs) with surfaces functionalized using polyacrylic acid (PAA) and hydrogen (H2O2) demonstrated a synergistic effect when combined X-ray irradiation. The combination generated H2O2 reactive oxygen species (ROS) that enhanced the irradiation efficacy. present study, we examined relationship between catalase PAA-TiOx NPs sensitization to radiation because is primary antioxidant enzyme converts water oxygen. Catalase-knockout PANC-1...
Cross-coupling reactions between short-lifetime radicals are challenging in organic chemistry. Here, we report the development of an N-heterocyclic carbene (NHC)-mediated radical coupling reaction based on catalytic machinery thiamine- and flavin-dependent enzymes. Through a series enzyme screenings, found that acetolactate synthase from Thermobispora bispora (TbALS) its engineered variants exhibit promising activity toward abiotic acylation α-bromo carbonyl compounds. Notably, TbALS variant...
Alpha (α)-glucosyl rutin (Rutin-G) was modified to the surface of titanium dioxide (TiO2) nanoparticles (NPs) with hydrogen peroxide (H2O2) improve accumulation TiO2 NPs in cancer cells. Rutin-G acts as a competitive inhibitor glucose and is hypothesized bind transporter 1 (Glut1), leading selective internalization Rutin-G-modified-NPs (RutinG-TiOx NPs). RutinG-TiOx were characterized for its optical properties using UV-Vis spectroscopy. Chemical bonds functional groups determined Fourier...
Styrene is an important industrial chemical. Although several studies have reported microbial styrene production, the amount of produced in batch cultures can be increased. In this study, was using genetically engineered Escherichia coli. First, we evaluated five types phenylalanine ammonia lyases (PALs) from Arabidopsis thaliana (AtPAL) and Brachypodium distachyon (BdPAL) for their ability to produce trans-cinnamic acid (Cin), a precursor. AtPAL2-expressing E. coli approximately 700 mg/L...
Abstract The demand for the essential commodity chemical 1,2‐propanediol (1,2‐PDO) is on rise, as its microbial production has emerged a promising method sustainable supply. However, reliance of 1,2‐PDO in Escherichia coli anaerobic conditions, enhancing cell growth to augment precursor availability remains substantial challenge. This study presents glucose‐based aerobic 1,2‐PDO, with xylose utilization facilitating growth. An engineered strain was constructed capable exclusively producing...
The pharmacological effects of Oren-gedoku-to (OGT), a Japanese-Chinese traditional herbal medicinal mixture on lipid biosynthesis were investigated in cultured human hepatocyte HepG2 cells. addition OGT (0.5 and 4.2 mg/ml), which had no effect cell proliferation cellular protein content, caused marked decrease the cholesterol particularly cholesteryl ester content following 24 h incubation. incorporation 14C-oleate into fraction was also reduced remarkably during incubation for 6 h. OGT,...
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