A5008091305- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Acute Lymphoblastic Leukemia research
- Cancer Genomics and Diagnostics
- Hematopoietic Stem Cell Transplantation
- CAR-T cell therapy research
- Immunodeficiency and Autoimmune Disorders
- Chronic Myeloid Leukemia Treatments
- Genomic variations and chromosomal abnormalities
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Prenatal Screening and Diagnostics
- Glycosylation and Glycoproteins Research
- Parvovirus B19 Infection Studies
- Protein Tyrosine Phosphatases
- Bone and Dental Protein Studies
- Helicobacter pylori-related gastroenterology studies
- Autoimmune and Inflammatory Disorders Research
- Viral Infectious Diseases and Gene Expression in Insects
- Erythrocyte Function and Pathophysiology
- Digestive system and related health
- Blood groups and transfusion
- Lung Cancer Treatments and Mutations
- Innovative Microfluidic and Catalytic Techniques Innovation
Shinshu University
2015-2025
Government of Western Australia Department of Health
2024
Ōtani University
1972-2022
Shinshu University Hospital
2010-2019
Nagasaki Medical Center
2016
Osaka University
2016
Johns Hopkins University
2015
Seiko Holdings (Japan)
2015
Mayo Clinic in Arizona
2015
University of California San Francisco Medical Center
2015
Large granular lymphocyte leukemia (LGL L) has been morphologically characterized as a group of lymphoproliferative diseases that include T-cell large lymphocytic (T-LGL and chronic disorders natural killer cells (CLPD-NK). We investigated mutations in the Src homology 2 (SH2) domain signal transducer activator transcription 3 (STAT3) gene Asian cohorts T-LGL L CLPD-NK (n = 42 11, respectively). Two mutations, Y640F D661Y, were identified using direct sequencing or allele-specific (AS) PCR....
Adoptive T cell therapy using chimeric antigen receptor (CAR)-modified cells is a promising cancer immunotherapy. We previously developed non-viral method of gene transfer into piggyBac transposon system to improve the cost-effectiveness CAR-T therapy. Here, we have further improved our technology by novel culture strategy increase transfection efficiency and reduce time manufacturing. Using CH2CH3-free CD19-specific CAR vector combining irradiated activated (ATCs) as feeder virus-specific...
Juvenile myelomonocytic leukemia (JMML) is a fatal, myelodysplastic/myeloproliferative neoplasm of early childhood. Patients with JMML have mutually exclusive genetic abnormalities in granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor (GMR, CD116) signaling pathway. Allogeneic hematopoietic stem cell transplantation currently the only curative treatment option for JMML; however, disease recurrence major cause failure. We investigated adoptive immunotherapy using GMR-targeted...
Abstract Only a few human ovarian endometrioid carcinoma cell lines are currently available, partly due to the difficulty of establishing from low-grade cancers. Here, using immortalization strategy consisting i) inactivation p16 INK4a -pRb pathway by constitutive expression mutant cyclin-dependent kinase 4 (R24C) (CDK4 R24C ) and cyclin D1, ii) acquisition telomerase reverse transcriptase (TERT) activity, we established line 46-year-old Japanese woman. That line, designated JFE-21, has...
Claudin 18 (CLDN18) is a member of the claudin family cell surface proteins, which are widely expressed in epithelial cells and play role cell-cell adhesion. CLDN18 isoform 2 (CLDN18.2) specifically gastric cells, frequently at high levels adenocarcinoma. On basis this, zolbetuximab, targeted monoclonal antibody, has been developed for patients with CLDN18.2-positive gastro-oesophageal Colitis-associated colorectal adenocarcinomas (CACs) tend to lose intestinal markers show aberrant mucin...
To investigate the phenotypic characters of carcinoma cells and response gastric epithelial to Helicobacter pylori (H. pylori) infection using cell lines.Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used assess effect H. on mRNA levels transcription factors (SOX2 CDX2), mucin core proteins (MUC2, MUC5AC, MUC6), trefoil factor family peptides (TFF) (TFF1, TFF2, TFF3) in (AGS, MKN45, KATO III cells). ATCC 43504 its isogenic cag pathogenicity island (PAI) deleted...
High-density lipoprotein (HDL) has protective effects against the development of atherosclerosis; these include reverse cholesterol transport, antioxidant ability, and anti-inflammation. Myeloperoxidase (MPO) secreted by macrophages in atherosclerotic lesions generates tyrosyl radicals apolipoprotein A-I (apoA-I) molecules, inducing formation apoA-I/apoA-II heterodimers through tyrosine-tyrosine bond HDL. Functional characterization HDL oxidized MPO could provide useful information about...
Background KRAS and NRAS gene mutations are frequently observed in childhood leukemia. The objective of this study was to determine the frequency RAS association between other genetic aberrations Arab Asian children with acute lymphoblastic leukemia (ALL) or myeloid (AML). Methods Diagnostic samples 485 patients (<18 years) from Iraq Jordan were obtained, using Flinders Technology Associates filter papers. Polymerase chain reaction direct sequencing performed Japan. Results detected 86/318...
Abstract A human ovarian clear cell carcinoma line was established from a 46-year-old Japanese woman. That line, designated MTC-22, has proliferated continuously for over 6 months in conventional RPMI 1640 medium supplemented with 10% foetal bovine serum and been passaged 50 times. MTC-22 doubling-time is ~ 18 h, which much shorter than most lines reported to date. Morphologically, cells exhibit polygonal shapes proliferate form monolayer jigsaw puzzle-like arrangement without contact...
To evaluate minimal residual disease (MRD) after chemotherapy and haematopoietic stem cell transplantation in juvenile myelomonocytic leukaemia (JMML), a locked nucleic acid-allele specific quantitative polymerase chain reaction (LNA-AS-qPCR) was developed for 13 patients (four types of PTPN11 mutation four RAS mutation). The post-transplant MRD detected by LNA-AS-qPCR analysis well correlated with chimerism assessed short tandem repeat PCR analysis. Non-intensive exerted no substantial...