Yusuke Okuno
A5034233071- Acute Myeloid Leukemia Research
- Cancer Genomics and Diagnostics
- Immunodeficiency and Autoimmune Disorders
- Epigenetics and DNA Methylation
- Blood disorders and treatments
- Viral-associated cancers and disorders
- Glioma Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- DNA Repair Mechanisms
- Hematopoietic Stem Cell Transplantation
- Cardiac electrophysiology and arrhythmias
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- RNA modifications and cancer
- Parvovirus B19 Infection Studies
- Advanced NMR Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Blood groups and transfusion
- Hedgehog Signaling Pathway Studies
- Genomics and Rare Diseases
- Platelet Disorders and Treatments
- Skin and Cellular Biology Research
- Cancer-related gene regulation
Nagoya City University
2021-2025
Nagoya University Hospital
2016-2024
Mizuho (Japan)
2024
National Institute of Diabetes and Digestive and Kidney Diseases
2019-2023
Hokkaido University
2023
National Institutes of Health
2019-2023
Nagoya University
2013-2022
University of Wisconsin–Madison
2014-2019
The University of Tokyo
2005-2018
Kyoto University
1995-2017
High-throughput DNA sequencing significantly contributed to diagnosis and prognostication in patients with myelodysplastic syndromes (MDS). We determined the biological prognostic significance of genetic aberrations MDS. In total, 944 various MDS subtypes were screened for known/putative mutations/deletions 104 genes using targeted deep array-based genomic hybridization. 845/944 (89.5%) harbored at least one mutation (median, 3 per patient; range, 0-12). Forty-seven mutated TET2, SF3B1,...
In patients with acquired aplastic anemia, destruction of hematopoietic cells by the immune system leads to pancytopenia. Patients have a response immunosuppressive therapy, but myelodysplastic syndromes and acute myeloid leukemia develop in about 15% patients, usually many months years after diagnosis anemia.
Reactive aldehydes arise as by-products of metabolism and are normally cleared by multiple families enzymes. We find that mice lacking two aldehyde detoxifying enzymes, mitochondrial ALDH2 cytoplasmic ADH5, have greatly shortened lifespans develop leukemia. Hematopoiesis is disrupted profoundly, with a reduction hematopoietic stem cells common lymphoid progenitors causing severely depleted acquired immune system. show formaldehyde substrate ADH5 establish methods to quantify elevated blood...
Recent advances in high-throughput sequencing technologies have enabled a comprehensive dissection of the cancer genome clarifying large number somatic mutations wide variety types. A methods been proposed for mutation calling based on amount data, which is accomplished most cases by statistically evaluating difference observed allele frequencies possible single nucleotide variants between tumours and paired normal samples. However, an accurate detection remains challenge under low depths or...
Abstract Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma in childhood. Here we studied 60 RMSs using whole-exome/-transcriptome sequencing, copy number (CN) and DNA methylome analyses to unravel genetic/epigenetic basis of RMS. On methylation patterns, RMS clustered into four distinct subtypes, which exhibits remarkable correlation with mutation/CN profiles, histological phenotypes clinical behaviours. A1 A2 especially A1, largely correspond alveolar histology frequent PAX3/7...
The mammalian Hippo pathway is composed of Ste20-like (MST) kinases and large tumour suppressor (LATS) kinases. Upon the activation pathway, MST phosphorylate activate LATS kinases, which in turn transcriptional co-activators, yes-associated protein (YAP) co-activator with PDZ-binding motif (TAZ), recruit them to cytosol from nucleus off cell cycle-promoting anti-apoptotic gene transcriptions. Thus, restricts overgrowth prevents tumourigenesis. Although a high density stress signallings are...
Rad52 promotes the annealing of complementary strands DNA bound by replication protein A (RPA) during discrete repair pathways. Here, we used a fluorescence resonance energy transfer (FRET) between two fluorescent dyes incorporated into substrates to probe mechanism which human (hRad52) interacts with and mediates ssDNA–hRPA complexes. Human ssDNA or complex in two, concentration-dependent modes. At low hRad52 concentrations, was wrapped around circumference ring, while at higher stretched...
Purpose Acute lymphoblastic leukemia (ALL) makes up a significant proportion of all pediatric cancers, and relapsed ALL is leading cause cancer-associated deaths in children. Identification risk factors druggable molecular targets can lead to better stratification treatments subsequent improvement prognosis. Patients Methods We enrolled 59 children with or primary refractory who were treated our institutions. primarily performed RNA sequencing (RNA-seq) using patients’ leukemic cells...