A5103123008- Ion Transport and Channel Regulation
- Neuroscience of respiration and sleep
- Developmental Biology and Gene Regulation
- Neuroendocrine regulation and behavior
- Renal and related cancers
- Neuroblastoma Research and Treatments
- RNA and protein synthesis mechanisms
- Entomopathogenic Microorganisms in Pest Control
- Acute Lymphoblastic Leukemia research
- Neonatal Respiratory Health Research
- Streptococcal Infections and Treatments
- ATP Synthase and ATPases Research
- Silkworms and Sericulture Research
- Genomics and Chromatin Dynamics
- Ion channel regulation and function
- Olfactory and Sensory Function Studies
- Cancer therapeutics and mechanisms
- RNA Research and Splicing
- Forest Insect Ecology and Management
- Acute Myeloid Leukemia Research
- Hormonal Regulation and Hypertension
- Pancreatic function and diabetes
- Hemophilia Treatment and Research
- Sleep and Wakefulness Research
- Lymphoma Diagnosis and Treatment
Jichi Medical University
2013-2024
Kagoshima City Hospital
2006-2022
Institute of Macromolecular Chemistry
2016
Tochigi Medical Center
2010
Shimane University
2009
Jichi Medical University Hospital
2006
Kagoshima University
1991-2004
Howard Hughes Medical Institute
2004
Massachusetts Institute of Technology
2004
Dana-Farber Cancer Institute
2004
Drosophila sine oculis and eyes absent genes synergize in compound-eye formation. The murine homologues of these genes, Six Eya, respectively, show overlapping expression patterns during development. We hypothesized that Eya proteins cooperate to regulate their target genes. Cotransfection assays were performed with various combinations assess effects on a potential natural target, myogenin promoter, synthetic the thymidine kinase gene promoter fused multimerized Six4 binding sites. A clear...
The enhancer-binding factor NF-kappa B, which is found only in cells that transcribe immunoglobulin light chain genes, has been purified from nuclear extracts of Namalwa (human Burkitt lymphoma cells) by sequence-specific DNA affinity chromatography. B identified as a 51-kDa polypeptide UV-crosslinking analysis. "Footprint" and methylation-interference analyses have shown binding activity specific for the kappa enhancer sequence. activated vitro transcription human immunodeficiency virus...
Six1 is a member of the Six family homeobox genes, which function as components Pax-Six-Eya-Dach gene network to control organ development. expressed in otic vesicles, nasal epithelia,branchial arches/pouches, nephrogenic cords, somites and limited set ganglia. In this study, we established Six1-deficient mice found that development inner ear, nose, thymus, kidney skeletal muscle was severely affected. embryos were devoid ear structures, including cochlea vestibule, while their endolymphatic...
Homeobox genes constitute a large family of transcription factors that are essential during normal development and often dysregulated in cancer. However, the molecular mechanisms by which homeobox influence cancer remain largely unknown. Here we show tissue-restricted cyclin A1 is transcriptional target Six1 homeoprotein. Both expressed embryonic but not terminally differentiated mammary gland, Six1-knockout mice dramatic reduction gland. In addition, both reexpressed breast cancers....
ABSTRACT The Drosophila homeobox gene sine oculis is expressed in the rostral region of embryo early development and essential for eye brain formation. Its murine homolog, Six3, anterior neural plate anlage, may have crucial functions development. In this study, we describe cloning expression zebrafish six3, apparent ortholog mouse Six3 gene. Zebrafish six3 transcripts are first seen hypoblast cells gastrula embryos found axial mesendoderm through gastrulation. head ectoderm begins at late...
Muscle fibers show great differences in their contractile and metabolic properties. This diversity enables skeletal muscles to fulfill adapt different tasks. In this report, we that the Six/Eya pathway is implicated establishment maintenance of fast-twitch muscle phenotype. We demonstrate MEF3/Six DNA binding element present aldolase A pM promoter mediates high level activation glycolytic (but not slow-twitch) fibers. also among Six Eya gene products expressed mouse muscle, Six1 Eya1...
We have isolated a cDNA clone for the β-subunit of HeLa cell Na, K–ATPase, containing 2208-base- pair insert covering whole coding region β-subunit. Nucleotide sequence analysis revealed that amino acid human K–ATPase exhibited 61% homology with Torpedo counterpart (Noguchi et al. (1986) FEBS Lett . in press). A remarkable conservation nucleotide 3' non–coding was detected between and cDNAs. RNA blot hybridization presence two mRNA species cells. S1 nuclease mapping indicated they were...
Phox2b protein is a specific marker for neurons in the parafacial region of ventral medulla, which are proposed to play role central chemoreception and postnatal survival. Mutations PHOX2B cause congenital hypoventilation syndrome. However, there have been no reports concerning electrophysiological characteristics these Phox2b-expressing neonate immediately after birth. This overlaps with respiratory group (pFRG) composed predominantly preinspiratory (Pre-I) that involved rhythm generation....
We identified five cDNA clones of the Six gene family which are expressed in retina. They Six2, Six3α and Six3β (which derived from alternative splicing forms), Six5, AREC3/Six4. All these genes possess extensive sequence similarity among each other so ‐homologous region ( domain homeodomain) but differ greatly structure some regions. The amino acid so‐homologous to previously AREC3/Six4 is 70.1% for 57.3% Six3β, 70.3% Six5. expression was observed inner outer nuclear layer, ganglion cell...
Drosophila sine oculis, eyes absent, and dachshund are essential for compound eye formation form a gene network with direct protein interaction genetic regulation. The vertebrate homologues of these genes, Six, Eya, Dach, also similar during muscle formation. To elucidate the molecular mechanism underlying among we examined interactions encoded proteins. Eya interacted directly Six but never Dach. Dach transactivated multimerized GAL4 reporter by coproduction GAL4-Eya fusion Transactivation...
In order to evaluate the protective role of maternal antibody against mother-to-child transmission HTLV-I, we followed a total 780 children born HTLV-I carrier mothers by investigating level anti-HTLV-I transferred in utero, decline and seroconversion post-natal life. The was positively detected within first 3-6 months life declined at 6-12 after birth all children. After declined, occurred some following either breast feeding or bottle feeding. rates short-term (less than equal 6 months)...
Using the method of gene targeting in mouse embryonic stem cells, regulatory function δEF1, a zinc finger and homeodomain-containing transcription factor, was investigated vivo by generating δEF1 mutant mice. The mutated allele produced truncated form protein lacking cluster proximal to COOH terminus. homozygous mice had poorly developed thymi with no distinction cortex medulla. Analysis thymocyte showed reduction total cell number two orders magnitude accompanying impaired development....
Clones carrying cDNA sequences for the alpha-subunit of Na,K-ATPase from HeLa cells have been isolated. Nucleotide sequence analysis cloned has revealed primary structure this polypeptide, which consists 1,023 amino acids. The human exhibited 87% homology with its Torpedo counterpart and 98% sheep counterpart. six putative transmembrane segments M1-M6 showed higher conservation than total segments. Total genomic Southern hybridization indicated existence at most two copies, possibly only...
The sodium pump is the enzyme responsible for maintenance of Na+ and K+ gradients across cell membrane. Four isoforms catalytic alpha subunit have been identified, but their individual roles remain essentially unknown. To investigate necessary functions alpha2 in vivo, we generated analyzed mice defective gene. Mice homozygous mutation died just after birth displayed selective neuronal apoptosis amygdala piriform cortex. In these regions, high expression c-Fos before indicated neural...
DNA complementary to the Torpedo californica electroplax mRNA coding for β‐subunit of (Na + K )‐ATPase has been cloned by screening a cDNA library with an oligodeoxyribonucleotide probe. Nucleotide sequence analysis revealed that this polypeptide consists 305 amino acid residues (including initiating methionine). The transmembrane topology and potential N ‐glycosylation sites are discussed.
Na+, K+-ATPase 2 subunit gene (Atp1a2) knock-out homozygous mice (Atp1a2-/-) died immediately after birth resulting from lack of breathing. The respiratory-related neuron activity in Atp1a2-/- was investigated using a brainstem-spinal cord en bloc preparation. respiratory motoneuron recorded the fourth cervical ventral root (C4) defective fetuses embryonic day 18.5. C4 response to electrical stimulation ventrolateral medulla (VLM) recovered more slowly than wild type during superfusion with...
Highlights•Loss of Six1/Six4 leads to impaired Sry expression and gonadal size reduction•Sry transgene rescued male differentiation but not initial precursor cell growth•Six1/Six4 regulate Fog2, which is a direct regulator expression•Six1/Six4 Nr5a1, controls gonad in an independent pathwaySummaryThe Y-linked gene regulates mammalian sex determination bipotential embryonic gonads. Here, we report that the transcription factors Six1 Six4 are required for differentiation. Loss together,...