A5091477080- Neuroendocrine regulation and behavior
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Retinal Development and Disorders
- Leprosy Research and Treatment
- Tuberculosis Research and Epidemiology
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Mycobacterium research and diagnosis
- Crystallization and Solubility Studies
- Cellular transport and secretion
- Signaling Pathways in Disease
- Stress Responses and Cortisol
- X-ray Diffraction in Crystallography
- Growth Hormone and Insulin-like Growth Factors
- Regulation of Appetite and Obesity
- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- Organometallic Complex Synthesis and Catalysis
- Electrolyte and hormonal disorders
- Biomedical Ethics and Regulation
- Oxidative Organic Chemistry Reactions
- Thyroid Disorders and Treatments
- Biochemical Analysis and Sensing Techniques
- Pharmacological Effects and Toxicity Studies
National Center For Child Health and Development
2015-2025
Saitama University
2002-2024
Kyoto University
1995-2021
Duquesne University
2021
Okayama University
2020
National Institute of Infectious Diseases
2008-2014
Konica Minolta (Japan)
2013
Kyushu University Hospital
2012
Jichi Medical University
2003-2012
National Research Institute
2012
Glaucoma, a progressive optic neuropathy due to retinal ganglion cell (RGC) degeneration, is one of the leading causes irreversible blindness. Although glaucoma often associated with elevated intraocular pressure (IOP), IOP elevation not detected in significant subset glaucomas, such as normal tension (NTG). Moreover, some patients, reduction does prevent progression disease. Thus, understanding IOP-independent mechanisms RGC loss important. Here, we show that mice deficient glutamate...
Thiol redox status modulates various aspects of cellular function. We demonstrate that oxidation sulfhydryl (SH) groups induces apoptosis. In Jurkat T cells and human PBL blasts, the fraction apoptotic nuclei increased after treatment with an SH-specific oxidant, diamide. Analysis DNA fragmentation nuclear morphology also indicated SH could induce apoptosis induced by oxidation, decrease glutathione was transient increase disulfide observed only changes had occurred. Depletion buthionine...
Six1 is a member of the Six family homeobox genes, which function as components Pax-Six-Eya-Dach gene network to control organ development. expressed in otic vesicles, nasal epithelia,branchial arches/pouches, nephrogenic cords, somites and limited set ganglia. In this study, we established Six1-deficient mice found that development inner ear, nose, thymus, kidney skeletal muscle was severely affected. embryos were devoid ear structures, including cochlea vestibule, while their endolymphatic...
Glia, the support cells of central nervous system, have recently attracted considerable attention both as mediators neural cell survival and sources regeneration. To further elucidate role glial in neurodegeneration, we generated TrkBGFAP TrkBc-kit knockout mice which TrkB, a receptor for brain-derived neurotrophic factor (BDNF), is deleted retinal glia or inner neurons, respectively. Here, show that extent glutamate-induced degeneration was similar these two mutant mice. Furthermore mice,...
Cholangiocarcinoma (CCA) is a cancer arising from the neoplastic transformation of cholangiocytes. During tumorigenesis, tumor suppressor and cancer-related genes are commonly silenced by aberrant DNA methylation in their promoter regions. Zebularine (1-(β-D-ribofuranosyl)-1,2-dihydropyrimidin-2-one) acts as an inhibitor exhibits chemical stability minimal cytotoxicity both vitro vivo. In this study, we explore effect possible mechanism action zebularine on CCA cells. We demonstrate that...
Ecalectin/galectin-9 was recently described as a novel eosinophil chemoattractant highly expressed in immune tissues. We investigated the regulation of galectin-9 expression and release Jurkat (a T cell line) cells. demonstrated that medium long-sized isoforms were constitutively expressed, phorbol 12-myriastate 13-acetate (PMA) upregulated level mRNA Western blotting flow cytometry analyses revealed PMA stimulation resulted upregulation both intracellular surface protein. The stimulated...
The human colon cancer-derived cell line Caco-2 is widely used in drug discovery due to its barrier function and transporter activity. However, cells have extremely low metabolic capacity, resulting discrepancies with physiology. In this study, we conducted experiments on intestinal epithelial generated from pluripotent stem cell-derived organoids. We assessed morphology, gene expression, functions, cytotoxicity relation growth the effects of cellular aging. results indicate that...
Ghrelin, a novel growth hormone (GH)-releasing peptide, was recently isolated from the rat stomach as an endogenous ligand to secretagogue receptor (GHS-R). Ghrelin specifically stimulates release of GH anterior pituitary gland, but regulational effect ghrelin on secretion has not yet been clarified. We used perifusion system examine single and combined effects with hormone-releasing (GHRH) somatostatin cells. The increase in concentration due stimulation showed transitory peak that almost...
Mycobacterium leprae survives and replicates within a lipid droplet stored in the enlarged phagosome of histiocytes, typical feature lepromatous leprosy that is thought to be an important nutrient source for bacillus. However, underlying mechanisms by which lipids accumulate phagosomes remain unclear. Recently, it was revealed droplet-associated proteins, including ADRP perilipin, play essential roles accumulation adipocytes or macrophages. Therefore, we attempted examine role these proteins...
Hepatocellular carcinoma is one of the most common cancers worldwide. During tumorigenesis, tumor suppressor and cancer-related genes are commonly silenced by aberrant DNA methylation in their promoter regions. Zebularine (1-(β-D-ribofuranosyl)-1,2-dihydropyrimidin-2-one) acts as an inhibitor exhibits chemical stability minimal cytotoxicity both vitro vivo. In this study, we explore effect possible mechanism action zebularine on hepatocellular cell line HepG2. We demonstrate that antitumor...
Exposure to arsenic from well water in developing countries is suspected cause developmental neurotoxicity. Although, it has been demonstrated that exposure sodium arsenite (NaAsO2) suppresses neurite outgrowth of cortical neurons vitro, largely unknown how NaAsO2 impairs higher brain function and affects histology. Here, we investigated the effect prenatal on behavior mice adulthood, evaluated histological changes prelimbic cortex (PrL), which a part medial prefrontal critically involved...
This study assesses the safety and efficacy of hepatocyte-like cell (HLC) infusion therapy derived from human embryonic stem cells as bridging for neonatal-onset urea cycle disorders (UCD). The research includes both preclinical clinical evaluations to determine feasibility HLC a therapeutic option safer pediatric liver transplantation. Preclinical studies were conducted validate safety, biodistribution, ammonia metabolism capabilities HLCs using SCID mice models UCD extensive animal...
Zn(II), Cd(II), Co(II) and Mg(II) binding to apoalkaline phosphatase of Escherichia coli the relative stabilities resulting metalloenzyme complexes have been measured by equilibrium dialysis metal exchange reactions using gamma-emitting isotopes these metals. At millimolar concentrations ions alkaline dimer binds three pairs (A, B, C sites). One dialyzes readily without detectable change in structure or function enzyme (C site). Of remaining two pairs, affinity both for Zn(II) Cd(II) is...
Abstract Charcot‐Marie‐Tooth (CMT) disease is the most frequent peripheral neuropathy affecting Schwann cells and neurons. CMT type 2 (CMT2) neuropathies are characterized by nerve aberrance. Four missense mutations of Rab7, a small GTPase Rab family involved in intracellular vesicular trafficking, associated with CMT2B phenotype. Despite growing body evidence concerning gene structures responsible for genetically heterogenous other CMT2 neuropathies, little known about vitro model how...
The Nanoculture plate (NCP) is a recently developed which essentially consists of textured surface with specific characteristics that induce spheroid formation: microfabrications micro-square pattern on the culture surface. NCP can be used to generate uniform adhesive spheroids cancer cell lines using conventional techniques without need any animal compounds. In this study, we assessed performance human hepatoma line HepG2 cells cultured an evaluate effects their hepatocyte-specific...