A5070886551- Mycobacterium research and diagnosis
- Leprosy Research and Treatment
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Infectious Diseases and Mycology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- HIV Research and Treatment
- Fungal Infections and Studies
- Dermatological diseases and infestations
- Pharmacological Effects of Natural Compounds
- Monoclonal and Polyclonal Antibodies Research
- Autoimmune and Inflammatory Disorders
- Hematological disorders and diagnostics
- Asthma and respiratory diseases
- interferon and immune responses
- Thyroid Disorders and Treatments
- Dermatology and Skin Diseases
- Herpesvirus Infections and Treatments
- Vector-borne infectious diseases
- RNA Interference and Gene Delivery
- Viral Infections and Vectors
- Antimicrobial Peptides and Activities
National Institute of Infectious Diseases
2014-2024
Zen-Noh (Japan)
2024
Kitamurayama Hospital
2022
Teikyo University
2018-2021
Murayama Medical Center
2018-2020
Hokkaido Pharmaceutical University
2018
Tokyo University of Agriculture
2018
Hokkaido University
2018
Yamaguchi University
2018
Yokohama City University
1994-2015
A DNA vaccine constructed with the CMV promoter conjugated to env gp160 and rev genes has been shown induce an effective Th1-type immune response when inoculated via intramuscular route. In present study, we obtained high levels of both humoral cell-mediated activity by intranasal administration this vaccine. The production mucosal IgA Ab in feces vaginal fluid was stimulated significantly administration. This route resulted a significant level HIV-1-neutralizing Abs serum. Cytokine assays...
Neglected tropical diseases (NTDs) are communicable that occur under conditions of poverty and concentrated almost exclusively in impoverished populations the developing world. NTDs affect more than 1000 million people subtropical countries, costing economies billions dollars every year. Effective control can be achieved with use large-scale delivery single-dose preventive chemotherapy (PC) or intensified disease management (IDM) both, as is case for some such lymphatic filariasis, trachoma,...
Abstract Previous investigations have demonstrated that CTL may play an important role in suppressing the disease progression of HIV infection. In this study, we inoculated mice with IL-12 expression plasmid together plasmid-encoding HIV-1 envelope to enhance activity by activating a Th1-type response. The results delayed-type hypersensitivity using footpad swelling response and clearly showed HIV-1-specific cell-mediated immunity was enhanced inoculation plasmid. Quantitation cytokine sera...
ABSTRACT Induction of mucosal and cell-mediated immunity is critical for development an effective vaccine against human immunodeficiency virus (HIV). We compared intramuscular intranasal immunizations with a DNA encoding env HIV-1 evaluated the QS-21 saponin adjuvant augmentation systemic immune responses to in murine model. Vaccination via two routes elicited comparable responses, consistently enhanced antigen-specific serum immunoglobulin G2a (IgG2a) production, delayed-type...
T cells from strains responder to the antigen poly(Glu40 Ala60) (GA) were depleted of alloreactive by bromo-deoxyuridine and light treatment, subsequently primed in vitro GA presented allogeneic macrophages nonresponder strains. Antigen-specific secondary proliferative responses restricted Ia molecules obtained all strain combinations tested. These data indicate that Ir gene-controlled nonresponsiveness cannot be result a failure presentation.
Abstract Mycobacterium ulcerans is the causative agent of Buruli ulcer, a neglected tropical skin disease that most commonly found in children from West and Central Africa. Despite severity infection, therapeutic options are limited to antibiotics with severe side effects. Here, we show M. susceptible anti-tubercular drug Q203 related compounds targeting respiratory cytochrome bc 1 :aa 3 . While primary terminal oxidase tuberculosis , presence an alternate bd-type limits bactericidal...
Background Early detection of several skin-related neglected tropical diseases (skin NTDs)–including leprosy, Buruli ulcer, yaws, and scabies- may be achieved through school surveys, but such an approach has seldom been tested systematically on a large scale in endemic countries. Additionally, better understanding the spectrum skin at-risk populations to encountered during surveys is necessary facilitate process. Methods We performed survey for selected NTDs diseases, among primary...
The adjuvant effect of mannan‐coated liposomes on human immunodeficiency virus type‐1 (HIV‐1) DNA vaccine and the mechanism this enhancement were studied. Coating cationic with mannan significantly enhanced ability to induce an HIV‐specific delayed‐type hypersensitivity (DTH) response. cytotoxic T‐cell (CTL) activity elicited by vaccination was also mannan‐liposome cocktail. This mannan‐liposome‐mediated greatly inhibited in vivo injection anti‐interferon (IFN)‐γ antibody, which suggests...
Liposomes have been widely used to enhance the immune response. In present investigation, we studied their in vivo immunomodulation of an HIV-1-specific DNA vaccine candidate (pCMV160/REV) constructed with cytomegalovirus (CMV) promoter-conjugated HIV-1 env and rev plasmids. By immunizing pCMV160/REV cationic liposomes through various routes (intramuscular, intraperitoneal, subcutaneous, intradermal, intranasal), induced higher levels both antibody production delayed-type hypersensitivity...
DNA vaccines have the potential of giving rise to a potent cell-mediated immune response by inducing intracellular synthesis and subsequent antigenic presentation encoded antigens. We tested vaccine specific for human immunodeficiency virus type 1 (HIV-1) injection animals with expression plasmids encoding HIV-1 envelope protein Rev regulatory protein. Injection both into mice, rabbits, or macaques was found induce high levels antibodies capable efficiently inhibiting infection...
Abstract B7 co‐stimulation is essential for activating resting T cells following antigen recognition by the cell receptor. To determine whether has adjuvant activities on human immunodeficiency virus type‐1 (HIV‐1)‐specific immunity induced inoculation of a plasmid encoding HIV‐1 env and rev (DNA vaccine), B7‐1 B7‐2 expression plasmids were co‐inoculated with DNA vaccine. The delayed‐type hypersensitivity response cytotoxic lymphocyte (CTL) activity significantly enhanced when was vaccine,...
Abstract To clarify the molecular basis of human TLR9 (hTLR9) gene expression, activity hTLR9 promoter was characterized using myeloma cell line RPMI 8226. Reporter analysis and EMSA demonstrated that transcription regulated via four cis-acting elements, cAMP response element, 5′-PU box, 3′-PU a C/EBP site, interacted with CREB1, Ets2, Elf1, Elk1, C/EBPα factors. Other members family, such as C/EBPβ, C/EBPδ, C/EBPε, were also important for transcription. CpG DNA-mediated suppression led to...
Toll-like receptors (TLR) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase play an essential role in intracellular eradication of engulfed pathogens. Here, we demonstrate the physical functional association between components cytosolic NADPH TLR-mediated signaling molecules. Cytosolic suppressed NF-kappaB activation as well IFN-beta promoter activation. We that TNF-associated factor (TRAF) 4 associates with p47(phox), a component oxidase, physically interacts functionally...
Buruli ulcer is a chronic skin disease caused by Mycobacterium ulcerans, which produces toxic lipid mycolactone. Despite the extensive necrosis and tissue damage, lesions are painless. This absence of pain prevents patients from seeking early treatment and, as result, many experience severe sequelae, including limb amputation. We have reported that mice inoculated with M. ulcerans show loss sensation nerve degeneration. However, molecules responsible for damage not been identified. In order...
The rapidly growing mycobacterium M. abscessus sensu lato is the causative agent of emerging pulmonary and skin diseases infections following cosmetic surgery postsurgical procedures. can be divided into at least three subspecies: subsp. abscessus, massiliense, bolletii. Clinical isolates mycobacteria were previously identified as by DNA-DNA hybridization. More than 30% these 117 clinical differentiated massiliense using combinations multilocus genotyping analyses. A much more cost-effective...