A5075326863- Mycobacterium research and diagnosis
- Infectious Diseases and Mycology
- Tuberculosis Research and Epidemiology
- Genomics and Phylogenetic Studies
- Infectious Diseases and Tuberculosis
- Ginseng Biological Effects and Applications
- Aquaculture disease management and microbiota
- Bacterial Identification and Susceptibility Testing
- Quinazolinone synthesis and applications
- Myxozoan Parasites in Aquatic Species
- Fungal Infections and Studies
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Microbial Natural Products and Biosynthesis
- Insect Utilization and Effects
- Veterinary medicine and infectious diseases
- Plant and fungal interactions
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Leprosy Research and Treatment
- Identification and Quantification in Food
- Inflammasome and immune disorders
- Blood groups and transfusion
- Fungal Biology and Applications
National Institute of Infectious Diseases
2017-2025
NTL Institute for Applied Behavioral Science
2022
Nippon Veterinary and Life Science University
2013-2018
Hokkaido Pharmaceutical University
2018
Teikyo University
2018
Presence of Mycobacterium fortuitum in respiratory tracts usually indicates mere colonization or transient infection, whereas true pulmonary infection occurs patients with gastroesophageal disease. However, little is known about the diagnostic indications for M. and natural history disease.A 59-year-old man was referred to our hospital treatment against infection. Fifteen years before referral, he underwent total gastrectomy, after which experienced esophageal reflux symptoms. After patient...
The rapidly growing mycobacterium M. abscessus sensu lato is the causative agent of emerging pulmonary and skin diseases infections following cosmetic surgery postsurgical procedures. can be divided into at least three subspecies: subsp. abscessus, massiliense, bolletii. Clinical isolates mycobacteria were previously identified as by DNA-DNA hybridization. More than 30% these 117 clinical differentiated massiliense using combinations multilocus genotyping analyses. A much more cost-effective...
The health burden of waterborne nontuberculous mycobacteria (NTM) is a rising concern. While the water supply systems can serve as potential reservoir for NTM, their abundance, diversity, and transmission pathways remain unknown. This study aimed to characterize prevalence regrowth NTM in building system hospital where many M. abscessus were isolated from patients. depletion residual chlorine after stagnation warm at point use promoted significant microbial regrowth, including hospital....
ABSTRACT Mycobacterium ulcerans , a slow-growing nontuberculous mycobacterium, causes Buruli ulcer, neglected tropical disease. Distinguishing M. from related species, including marinum poses challenges with respect to making accurate identifications. In this study, we developed rapid and simple identification method based on mycobacterial lipid profiles used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analyze the of ( n = 35) 19) isolates....
The clinical impact of infection with Mycobacterium (M.) abscessus complex (MABC), a group emerging non-tuberculosis mycobacteria (NTM), is increasing. M. subsp. abscessus/bolletii frequently shows natural resistance to macrolide antibiotics, whereas massiliense generally susceptible. Therefore, rapid and accurate discrimination macrolide-susceptible MABC subgroups required for effective decisions about treatments infection. We aimed develop simple diagnostic that can identify isolates...
A previously undescribed rapidly growing, non-pigmented mycobacterium was identified based on biochemical and nucleic acid analyses, as well growth characteristics. Seven isolates were cultured from samples collected five thread-sail filefish (Stephanolepis cirrhifer) two farmed black scraper (Thamnaconus modestus). Bacterial occurred at 15-35 °C Middlebrook 7H11 agar. The bacteria positive for catalase activity 68 urease activity, intermediate iron uptake, negative Tween 80 hydrolysis,...
Sitafloxacin (STFX) exhibits potent activity against Mycobacterium avium complex (MAC) in both vitro and vivo experiments. However, limited data are available for the clinical efficacy adverse effects of STFX susceptibility refractory MAC lung disease (MAC-LD) to drug. Therefore, this study was aimed at evaluating safety an STFX-containing regimen treatment MAC-LD.We retrospectively evaluated outcomes 31 patients with MAC-LD, who received ≥4 weeks between January 2010 July 2017. Refractory...
Through genome mining for fungal macrolide natural products, we discovered a characteristic family of putative biosynthetic gene clusters that contain glycosylphosphatidylinositol–ethanolamine phosphate transferase (GPI-EPT) homologue. the heterologous expression two from Aspergillus kawachii and Colletotrichum incanum, new macrolides, including those with phosphoethanolamine or phosphocholine moieties, were formed. This study is first demonstration tailoring steps catalyzed by GPI-EPT...
Mycobacterium ulcerans is the causative agent of Buruli ulcer (BU), a WHO-defined neglected tropical disease. All Japanese BU isolates have shown distinct differences from prototype and are categorized as M. subspecies shinshuense. During repeated sub-culture, we found that some shinshuense colonies were non-pigmented whereas others pigmented. Whole genome sequence analysis revealed did not harbor giant plasmid, which encodes elements needed for mycolactone toxin biosynthesis. Moreover, was...
In 2009 and 2010, unusually high mortality events were recorded among cultured populations of thread-sail filefish Stephanolepis cirrhifer in Ehime Prefecture, Japan. Diseased fish exhibited abdominal distention many white nodules filled with thick, pale-yellow material scattered on the surface serosae internal organs mesentery. Histopathologically, disease was characterized by variable sized granulomatous lesions central necrotic core surrounded thin irregular arrangement epithelioid cells...
Mycolicibacterium phlei (M. phlei) is known to be a non-pathogenic nontuberculous mycobacterium (NTM) which rarely causes diseases in humans. A disseminated NTM infection mostly caused by the Mycobacterium avium complex (MAC) and develop immunocompromised hosts, like those with acquired immune deficiency syndrome (AIDS). Here, we report case of M. an immunocompetent host carrying anti-interferon gamma (IFN-γ) autoantibodies. We detected multiple organs elderly woman no significant medical...
Non-tuberculous mycobacteria (NTM) cause skin infections, respiratory diseases, and disseminated infections. Mycobacterium avium intracellulare, which are slow grown Mycobacterium, main agents of those NTM diseases. A silkworm infection model with abscessus, a rapidly growing species, was established to quantitatively evaluate its virulence within short period. However, the M. intracellulare has not yet been developed. In this study, we determined subspecies 4 days using model. The strains...
Among non-tuberculous mycobacteria (NTM), the Mycobacterium simiae complex is one of largest groups, consisting 18 species slow-growing mycobacteria. In 2009, a case NTM-associated infectious skin disease was reported in Shiga Prefecture, Japan. The patient presented with scattered nodules on chest, back and extremities, an M. simiae-like organism isolated from biopsy specimens obtained these lesions. Based several assessments, including multiple-gene analyses, biochemical characterization...
Mycobacterium abscessus causes chronic skin infections, lung diseases, and systemic or disseminated infections. Here we investigated whether the virulence of M. clinical isolates could be evaluated by calculating median lethal dose (LD50) in a silkworm infection model. subsp. cells were injected into hemolymph. When reared at 37˚C, silkworms died within 2 days post-infection with abscessus. Viable cell numbers increased hemolymph Silkworms not killed injections heat-killed cells. The...
Abstract Mycobacterium marinum is a slow‐growing, photochromogenic nontuberculous mycobacterium, which can cause mycobacteriosis in various animals, including humans. Several cases of fish have been reported to date. has also isolated from aquatic environmental sources such as water, sand, biofilms, and plants the natural environments. Hence, we hypothesized that wide variety could be involved transmission M. . In this study, tested hypothesis by isolating fish, invertebrates, seagrass,...
Mycobacterium marinum is a slowly growing, broad-host-range mycobacterial species. Here, we report the complete genome sequence of type strain that was isolated from tubercles diseased fish. This will provide essential information for future taxonomic and comparative studies its relatives.
Mycobacterium stephanolepidis is a rapid-growing nonpigmented species isolated from marine teleost fish (Stephanolepis cirrhifer) and closely related to chelonae Here, we report the complete sequence of its genome, comprising 4.9-Mb chromosome. The represents essential data for future phylogenetic comparative genome studies this pathogen.
Mycobacterium abscessus subsp. bolletii is a rapidly growing mycobacterial organism for which the taxonomy unclear. Here, we report complete genome sequence of type strain. This will provide essential information future taxonomic and comparative studies these mycobacteria.
Abstract In September 2014, a freshwater oomycete was first isolated from Asian Seabass Lates calcarifer fry that were reared in at fish hatchery Sabah, Malaysia. A fungal strain infected by using glucose yeast extract (GY) agar. From morphological identification, the belonged to genus Achlya based on mode of zoospore release. Molecular phylogenetic analysis internal transcribed spacer region sequences showed high similarity (99–100%) oblongata. The isolate able grow GY agar incubated...
Mycobacterium montefiorense is a member of the simiae complex, largest group nontuberculous mycobacteria. Here, we report genome sequence M. isolate BS, isolated from diseased Japanese black salamander (Hynobius nigrescens) reared in an aquarium Japan. This first reported case infection amphibian.
We herein report a 59-year-old woman with 2-year history of chronic bursitis the hand who took 50 mg/day prednisolone for several autoimmune diseases. Mycobacteroides abscessus subsp. massiliense was isolated from abscess and blood culture. Combination therapy (imipenem/cilastatin, amikacin, clarithromycin) administered month. Two months later, M. detected culture again, disseminated lesions were found. Clarithromycin sitafloxacin following eight weeks same regimen. Six after diagnosis,...