A5101726020- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Leprosy Research and Treatment
- Infectious Diseases and Mycology
- Infectious Diseases and Tuberculosis
- Fungal Infections and Studies
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Immune responses and vaccinations
- Immunodeficiency and Autoimmune Disorders
- Ginseng Biological Effects and Applications
- Microbial Natural Products and Biosynthesis
- Cancer-related molecular mechanisms research
- Quinazolinone synthesis and applications
- Actinomycetales infections and treatment
- Redox biology and oxidative stress
- Pharmacological Effects of Natural Compounds
- Nitric Oxide and Endothelin Effects
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- Immune Cell Function and Interaction
- Microbial Metabolism and Applications
- Escherichia coli research studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
National Institute of Infectious Diseases
2016-2025
Teikyo University
2018
Hokkaido Pharmaceutical University
2018
National Institutes of Health
2004-2011
Czech Academy of Sciences, Institute of Microbiology
2010
Nara University
2008
Osaka University
2003
Hiroshima University
1998
ObjectivesAntimicrobial resistance (AMR) is a priority for surveillance in bacterial infections. For leprosy, AMR has not been assessed because Mycobacterium leprae does grow vitro. We aim to obtain data using molecular detection of genes and conduct prospective open survey antileprosy drugs countries where leprosy endemic through WHO network.MethodsFrom 2009 2015, multi-bacillary cases at sentinel sites 19 were studied rifampicin, dapsone ofloxacin by PCR sequencing the...
Leprosy is a chronic human disease caused by the yet-uncultured pathogen Mycobacterium leprae. Although readily curable with multidrug therapy (MDT), over 200,000 new cases are still reported annually. Here, we obtain M. leprae genome sequences from DNA extracted directly patients' skin biopsies using customized protocol. Comparative and phylogenetic analysis of 154 genomes 25 countries provides insight into evolution antimicrobial resistance, uncovering lineages phylogeographic trends, most...
ABSTRACT Mycobacterium ulcerans , a slow-growing nontuberculous mycobacterium, causes Buruli ulcer, neglected tropical disease. Distinguishing M. from related species, including marinum poses challenges with respect to making accurate identifications. In this study, we developed rapid and simple identification method based on mycobacterial lipid profiles used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analyze the of ( n = 35) 19) isolates....
Multidrug therapy has effectively reduced the number of leprosy cases in world. However, rate reduction decelerated over years, giving early detection Mycobacterium leprae and epidemiological study relapse renewed relevance attempts to eliminate disease.A molecular survey for drug-resistant M. was conducted central highland regions Vietnam. A total 423 samples taken from patients, including 83 patients with new cases, 321 receiving treatment, 19 relapse, were studied mutations relating drug...
Although leprosy (Hansen's disease) is one of the oldest known diseases, pathogenicity Mycobacterium leprae (M. leprae) remains enigmatic. Indeed, cell wall components responsible for immune response against M. are as yet largely unidentified. We reveal here phenolic glycolipid-III (PGL-III) an leprae-specific ligand receptor Mincle. PGL-III a scarcely present trisaccharide intermediate in biosynthetic pathway to PGL-I, abundant and characteristic glycolipid. Using activity-based...
Buruli ulcer, caused by
Thioredoxin reductase (TR), a flavoprotein, catalyzes the reduction of oxidized thioredoxin in NADPH-dependent manner, and contains selenocysteine residue near C-terminus. TR plays an important role protecting against oxidative stress regulating cell growth death. Constitutive expression has been observed several types mammalian body, including endothelial cells. The latter are continually exposed to both exogenous endogenous sources nitric oxide (NO) NO-derived species. Reactive nitrogen...
Glycopeptidolipids (GPLs) are major components present on the outer layers of cell walls several nontuberculous mycobacteria. GPLs antigenic molecules and have variant oligosaccharides in mycobacteria such as Mycobacterium avium. In this study, we identified four genes (gtf1, gtf2, gtf3, gtf4) genome smegmatis. These were independently inactivated by homologous recombination M. smegmatis, structures from each gene disruptant analyzed. Thin-layer chromatography, gas chromatography-mass...
Mycobacterium ulcerans is the causative agent of Buruli ulcer (BU), a WHO-defined neglected tropical disease. All Japanese BU isolates have shown distinct differences from prototype and are categorized as M. subspecies shinshuense. During repeated sub-culture, we found that some shinshuense colonies were non-pigmented whereas others pigmented. Whole genome sequence analysis revealed did not harbor giant plasmid, which encodes elements needed for mycolactone toxin biosynthesis. Moreover, was...
Nutritional status contributes to the regulation of immune responses against pathogens, and malnutrition has been considered as a risk factor for tuberculosis (TB). Mycobacterium (Mtb), causative agent TB, can modulate host lipid metabolism induce accumulation in macrophages, where bacilli adopt dormant phenotype. In addition, serum components play dual roles protection from Mtb infection. We analyzed relationship between nutritional humoral response TB patients. found that HDL levels are...
Abstract A Japanese male in his 30s with no underlying medical condition presented painless nodules after being bitten by a dog during stay Bali, Indonesia, 7 years earlier. He was referred to our department multiple ulcers, nodules, and masses on the right leg. The final diagnosis mycetoma caused Nocardia vulneris , which may have been exacerbated colonization of Candida parapsilosis C. tropicalis as these yeasts were isolated culture from tissue. Treatment minocycline hydrochloride...
Mycobacterium avium complex (MAC) is a non-tuberculous mycobacterium widely distributed in the environment. Even though MAC infection increasing older women and immunocompromised patients, to our knowledge there has been no comprehensive analysis of MAC-infected host-cell transcriptome—and particularly long non-coding RNAs (lncRNAs). By using vitro- cultured primary mouse bone-marrow-derived macrophages (BMDMs) Cap gene expression, we analyzed transcriptional kinetic landscape macrophage...
We constructed a recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG-Delta UT) that lacks urease, providing acidic intraphagosomal conditions to drive an effective human immune T-cell response. BCG-Delta UT-infected macrophages stimulated autologous CD4+ T cells more efficiently than parent BCG-infected macrophages. For further activation, required pretreatment with exogenous granulocyte-macrophage colony-stimulating factor or costimulation either CD40 ligand interferon-gamma. By...
Abstract Mycobacterium lepromatosis , an independent species from leprae has been found to be a causative agent for diffuse lepromatous leprosy ( DLL ) in Mexico, but remains poorly studied. Here, the drug resistance‐determining regions DRDR of folP1, rpoB and gyrA (conferring resistance dapsone, rifampicin quinolone, respectively) M. patients Mexico were characterized. No mutations or silent at previously characterized major sites . However, non‐synonymous mutation was codon 54 between two...
Japan is one of the rare non-tropical countries with documented cases Buruli ulcer (BU). Mycobacterium ulcerans subsp. shinshuense has been identified as causative agent. The first report BU in dates back to 1982, sporadic reports thereafter. Recently, number on increase, and 50 (57.7%) are from past decade alone, out a total 87 reported date. Japan's well-developed healthcare facilities play crucial role enabling detailed investigations providing appropriate treatment for patients,...
Among non-tuberculous mycobacteria (NTM), the Mycobacterium simiae complex is one of largest groups, consisting 18 species slow-growing mycobacteria. In 2009, a case NTM-associated infectious skin disease was reported in Shiga Prefecture, Japan. The patient presented with scattered nodules on chest, back and extremities, an M. simiae-like organism isolated from biopsy specimens obtained these lesions. Based several assessments, including multiple-gene analyses, biochemical characterization...
A serological diagnostic test using phenolic glycolipid-I (PGL-I) developed in the 1980s is commercially available, but method still inefficient detecting all forms of leprosy. Therefore, more-specific and -reliable methods have been sought. We characterized major membrane protein II (MMP-II) as a candidate for new antigen. In this study, we evaluated effectiveness enzyme-linked immunosorbent assay (ELISA) MMP-II antigen (MMP-II ELISA) antibodies leprosy patients patients' contacts...
Mycobacterium bovis bacillus Calmette-Guérin (BCG) is the only tuberculosis (TB) vaccine currently available, but its efficacy against adult pulmonary TB remains controversial. BCG induces specific immune responses to mycobacterial antigens and may elicit protective immunity TB. a major public health problem, especially among elderly, yet of in elderly unknown. We investigated ability vaccination prevent young (6-week-old), middle-aged (18-month-old), old (60-month-old) guinea pigs....
Mycobacterium pseudoshottsii is a fish pathogen that produces mycolactone. Here, we report the complete chromosome sequence of type strain M. (JCM 15466). The will represent essential data for future phylogenetic and comparative genome studies mycolactone-producing mycobacteria.
Mycobacterium marinum is a slowly growing, broad-host-range mycobacterial species. Here, we report the complete genome sequence of type strain that was isolated from tubercles diseased fish. This will provide essential information for future taxonomic and comparative studies its relatives.
For the establishment of a diagnostic tool for mycobacterial species, part dnaA gene was amplified and sequenced from clinically relevant 27 species as well 49 clinical isolates. Sequence variability in segment allowed differentiation all except Mycobacterium tuberculosis, africanum microti, which had identical sequences. Partial sequences isolates belonging to three frequently isolated revealed very high intraspecies similarity, with range 96.0-100%. Based on sequences, species-specific...