ABSTRACT Human herpesvirus 6 (HHV-6), which belongs to the betaherpesvirus subfamily and infects mainly T cells in vitro, causes acute latent infections. Two variants of HHV-6 have been distinguished on basis differences several properties. We determined complete DNA sequence variant B (HHV-6B) strain HST, causative agent exanthem subitum, compared with that A U1102. total 115 potential open reading frames (ORFs) were identified within 161,573-bp contiguous entire genome, including some...

The presence of human herpesvirus 6 (HHV-6) and 7 (HHV-7) in throat swabs 62 children different age groups (group I, ages 0-5 month; group II, 6-11 months, III, 12-23 IV, 2-8 years) 28 adults was detected by polymerase chain reaction. detection rate HHV-6 DNA the highest (87%) aged 1-year-old decreased with age, whereas HHV-7 increased reached a maximum adults. HHV-6B almost all samples except for two who secreted only HHV-6A. When antibody prevalence determined four children, 8/12 (66.7%),...

To select adhesive strains among of Lactobacillus and to apply them inhibit adhesion food-borne pathogens.Twelve (10 from intestine) were examined for using Caco-2 cell cultures. The two most strains, crispatus JCM 8779 Lact. reuteri 1081, used test antiadhesion activity against enterotoxigenic Escherichia coli, Salmonella typhimurium Enterococcus faecalis strains. Adhesion the pathogens was inhibited by both Ent. especially strongly 8779. Although antimicrobial not detected in culture...

The complete nucleotide sequence of one the immediate-early genes human herpesvirus 6 variant B was determined and compared with that A reported by Martin et al. (M.D. Martin, J. Nicholas, B. Thomson, C. Newman, R. W. Honess, Virol. 65:5381-5390, 1991). While it two open reading frames exist in this region A, only frame found putative coding 2,679 nucleotides long. Furthermore, additive regions 108 228 bp were Primers covering these deleted synthesized used for PCR amplification. Twelve...

The increasing occurrence of multidrug-resistant Mycobacterium tuberculosis (Mtb) is a serious threat to global public health. Among the many mechanisms drug resistance, only resistance-nodulation-division (RND)-type multidrug efflux systems can simultaneously render bacteria tolerant numerous toxic compounds, including antibiotics. elevated expression RND-type xenobiotic transporter complexes, which consist an inner membrane transporter, fusion protein, and outer channel, plays major role...

Abstract The mode of transmission human herpesvirus 6 (HHV‐6) was investigated by molecular epidemiological techniques in four families. HHV‐6 isolated from the peripheral blood seven infants with exanthem subitum, including siblings, and saliva two mothers. These isolates were examined for genetic relatedness means restriction enzymes analysis purified DNA. While profiles digestion patterns differed among families, they very similar a particular family. results may suggest that is...

A search for an immunomodulating agent from mycobacteria was carried out using Mycobacterium leprae. The antigenicity of each fraction the bacterial membrane, which contains most antigenic components M. leprae, assessed by sera paucibacillary leprosy. N-terminal sequencing serum-reactive protein and functional assessment membrane fractions monocyte-derived dendritic cells (DCs) identified major II (MMP-II) as one efficient T-cell-activating candidates. Purified MMP-II stimulated DCs healthy...

Journal Article Salivacin 140, a novel bacteriocin from Lactobacillus salivarius subsp. salicinius T140 active against pathogenic bacteria Get access K. Arihara, Arihara School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada‐shi, Japan Search for other works by this author on: Oxford Academic Google Scholar S. Ogihara, Ogihara T. Mukai, Mukai M. Itoh, Itoh Y. Kondo Letters in Applied Microbiology, Volume 22, Issue 6, 1 June 1996, Pages 420–424,...

Partial sequences of the cytochrome b gene (402 bp) in mtDNA were determined for brackishwater gobiid fishes, genus Tridentiger, collected from geographically distant locations Japanese Archipelago, and their interspecific geographic variations analyzed compared. Contrary to results a previous allozyme analysis which revealed existence considerable genetic divergence (Nei's distance > 0.5) between T. obscurus brevispinis, haplotypes (mitotypes) these two species very similar could not be...

Four strains isolated from chicken small intestine and JCM 1038 1039 (designated as Lactobacillus acidophilus) were characterized by phenotypic molecular taxonomic methods. They Gram-positive, catalase-negative, facultatively anaerobic rods that did not produce gas glucose. These had similar characteristics exhibited intergroup DNA relatedness values of >77 %, indicating they comprised a single species. The 16S rRNA gene sequence representative strain, 1039(T) type strain in this study), was...

ABSTRACT Amino acid substitutions at position 89 or 91 in GyrA of fluoroquinolone-resistant Mycobacterium leprae clinical isolates have been reported. In contrast, those 94 M. tuberculosis , equivalent to 95 identified most frequently. To verify the possible contribution amino fluoroquinolone resistance, we conducted an vitro assay using wild-type and mutant recombinant DNA gyrases. Fluoroquinolone-mediated supercoiling activity inhibition cleavage revealed potent substitution Asp Gly Asn...

Yogurt is made by fermenting milk with 2 lactic acid bacteria, Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus. To comprehensively understand the protocooperation mechanism between S. thermophilus L. in yogurt fermentation, we examined 24 combinations of cocultures comprising 7 fast- or slow-acidifying strains 6 strains. Furthermore, 3 NADH oxidase (Nox)-deficient mutants (Δnox) one pyruvate formate-lyase deficient mutant (ΔpflB) were used to evaluate factor that...

Rabbit polyclonal antibody (Poly‐1) and mouse monoclonal antibodies (mAbs) TO‐11, TO‐14 TO‐M1 specific for Porphyromonas gingivalis 381 fimbriae were prepared. Poly‐1 the 3 mAbs screened their reactivity with whole cells of oral nonoral black‐pigmented bacterial species by enzyme‐linked immunosorbent assay (ELISA) binding experiment using [ 125 I] mAbs. ELISA revealed that definitely reacted all 11 strains P. tested. However, 8 TO‐M1. These results confirmed radiolabelled mAb TO‐11 to...

Glycopeptidolipids (GPLs) are major components present on the outer layers of cell walls several nontuberculous mycobacteria. GPLs antigenic molecules and have variant oligosaccharides in mycobacteria such as Mycobacterium avium. In this study, we identified four genes (gtf1, gtf2, gtf3, gtf4) genome smegmatis. These were independently inactivated by homologous recombination M. smegmatis, structures from each gene disruptant analyzed. Thin-layer chromatography, gas chromatography-mass...

In order to evaluate the biochemical characteristics of 14 substrains Mycobacterium bovis bacillus Calmette Guérin (BCG) - Russia, Moreau, Japan, Sweden, Birkhaug, Danish, Glaxo, Mexico, Tice, Connaught, Montreal, Phipps, Australia and Pasteur we performed eight different tests, including those for nitrate reduction, catalase, niacin accumulation, urease, Tween 80 hydrolysis, pyrazinamidase, p-amino salicylate degradation resistance thiophene 2-carboxylic acid hydrazide. Catalase activities...

Because Mycobacterium bovis bacillus Calmette-Guérin (BCG) unconvincingly activates human naive CD8(+) T cells, a rBCG (BCG-70M) that secretes fusion protein comprising BCG-derived heat shock (HSP)70 and leprae-derived major membrane (MMP)-II, one of the immunodominant Ags M. leprae, was newly constructed to potentiate ability activating cells through dendritic (DC). BCG-70M secreted HSP70-MMP-II in vitro, which stimulated DC produce IL-12p70 TLR2. BCG-70M-infected activated not only memory...

Summary. Human herpesvirus‐7 (HHV‐7), which is a newly identified human herpesvirus with an unknown pathologic role, was isolated from 5‐year‐old boy suffering fever, hepatosplenomegaly and pancytopenia. Although the clinical course similar to that of chronic active Epstein‐Barr virus infection, no viruses other than HHV‐7 were isolated. This finding raises possibility played pathogenic role in present patient.

Abstract Although human immunodeficiency virus type 1 (HIV‐1) subtypes C and E are expanding faster seem to be of greater global significance than HIV‐1 subtype B, there is only little information about Tat activity such non‐B subtypes. Here, we showed evidence that exhibits higher transcriptional from the long‐terminal repeat (LTR) in a T‐cell line, compared with B E. This was not due LTR, but sequence variability. We examined three candidate regions for Tat, as cysteine‐rich motif, basic...

Human herpesvirus 6 (HHV‐6) was isolated from patients with exanthem subitum (ES) a high frequency, and it is now believed that this virus causes ES as primary infection in childhood. HHV‐6 highly prevalent early childhood may infect infants through the saliva mainly mother to child. has tropism CD4+ cells destroys vitro. Although children recover without any sequelae, neurological symptoms associated are often observed, we could detect cerebrospinal fluid of patients. This result suggests...

Cynomolgus and African green monkeys were inoculated with human herpesvirus 6 (HHV-6). An antibody response was first observed 10 days 5 after inoculation of cynomolgus monkeys, respectively, detectable for the duration experiment (33 days). HHV-6 DNA detected by polymerase chain reaction in mononuclear cells one monkey virus inoculation, a total three four (75%) five (80%) later inoculation. Furthermore, lymph nodes spleen killed 33 A rash on trunk 13 otherwise infection asymptomatic. When...