A5017859001- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Genomics and Phylogenetic Studies
- Gene Regulatory Network Analysis
- Carbohydrate Chemistry and Synthesis
- Microbial Community Ecology and Physiology
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Infections and bacterial resistance
- Microbial Metabolic Engineering and Bioproduction
- Sphingolipid Metabolism and Signaling
- Protein Structure and Dynamics
- Neuroscience and Neural Engineering
- Bladder and Urothelial Cancer Treatments
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Plant Pathogenic Bacteria Studies
- Immune Response and Inflammation
- Electrochemical Analysis and Applications
- Probiotics and Fermented Foods
- Oral microbiology and periodontitis research
- HER2/EGFR in Cancer Research
- Synthesis and Characterization of Heterocyclic Compounds
- Sulfur-Based Synthesis Techniques
Kyushu Sangyo University
2004-2024
Toyota Memorial Hospital
2024
Asahikawa Medical University
2023
Higashi Osaka City General Hospital
2016-2022
Kōchi University
2021
Osaka City University
2004-2019
Tezukayama University
2019
Kogakuin University
2011
Japan BCG Laboratory
2001-2008
Saitama Women's Junior College
1992-2002
ErbB tyrosine kinase receptors mediate mitogenic signal cascade by binding a variety of ligands and recruiting the different cassettes adaptor proteins. In present study, we examined heregulin (HRG)-induced transduction ErbB4 receptor found that phosphatidylinositol 3'-kinase (PI3K)-Akt pathway negatively regulated extracellular signal-regulated (ERK) phosphorylating Raf-1 on Ser(259). As time-course kinetics Akt ERK activities seemed to be transient complex, constructed mathematical...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of disease 2019 (COVID-19), is an enveloped, single-stranded RNA virus. Favipiravir orally administrable antiviral drug whose mechanism action to selectively inhibit RNA-dependent polymerase. A preliminary trial in COVID-19 patients reported significant improvements across a multitude clinical parameters, but these findings have not been confirmed adequate well-controlled trial. We conducted randomized, single-blind,...
The 16S rDNA sequence similarities between the type strains of Sphingomonas paucimobilis and 32 other species range from 90.2 to 99.6%. It might be possible divide genus into several new genera according a dendrogram drawn similarity. However, phenotypic biochemical information needed define clusters representing distinct within this group organisms was not previously available. Although cellular lipids all 28 tested contained glucuronosyl-(1 --> 1)-ceramide together with 2-hydroxymyristic...
Four Gram-positive bacteria, strains A1-17B T , A1-22 A1-3 and A1-8, isolated from the air in Russian space laboratory Mir, were subjected to a polyphasic taxonomic study. Phylogenetic analysis of bacteria based on their 16S rDNA sequence showed that they belong genera Rothia (A1-17B ), Rhodococcus (A1-22 ) Arthrobacter (A1-3 A1-8). Morphological, physiological, chemotaxonomic genomic characteristics supported assignments these genera, but could not be classified as any existing species...
Based on the results of a phylogenetic analysis 16S rRNA and presence sphingoglycolipid in cellular lipids type strains, transfer "Rhizomonas" suberifaciens, Blastomonas natatoria Erythromonas ursincola to genus Sphingomonas as suberifaciens (van Bruggen et al 1990) comb. nov., (Sly 1985) (Yurkov 1997) nov. are herein proposed together with emendation Sphingomonas. The strain S. is van Cal=ATCC 49382=NCPPB 3629=IFO 15211=JCM 8521, that ATCC 35951 =DSM 3183=NCIMB 12085=JCM10396, DSM 9006= KR-99.
An efficient stereoselective synthesis of 4'-β-thioribonucleosides 14, 15, 27, and 30 using the Pummerer reaction as key step is described. The 1,4-anhydro-2-O-(2,4-dimethoxybenzoyl)-3,5-O-(1,1,3,3-tetraisopropyldisiloxane-1,3-diyl)-4-sulfinyl-d-ribitol (R-10:S-10 = 2.7:1) in presence silylated uracil afforded desired β-anomer 4'-thiouridine derivative 11 66% yield without formation its α-anomer. with R-10 gave 87% yield, while one S-10 resulted a 27% decrease product along 22%...
Abstract Based on the results of phylogenetic analysis 16S rDNA sequences and presence N ‐2′‐hydroxymyristoyl dihydrosphingosine 1‐glucuronic acid (SGL‐1) 2‐hydroxymyristic (non‐hydroxymyristic in Zymomonas ) cellular lipids, a new family, Sphingomonadaceae , for Group 4 alpha‐subclass class Proteobacteria is herein proposed description family given. The consists six genera, Sphingomonas, Erythrobacter, Erythromicrobium, Porphyrobacter, Sandaracinobacter . Thus, all validly published...
A polyphasic taxonomic study was performed on Sphingomonas sp. strain RW1T. The organism isolated from water of the River Elbe and has been known as a potent metabolizer dibenzo-p-dioxin its relatives. TLC mild alkaline hydrolysate extractable cellular lipids RW1T type strains 21 species gave spot sphingoglycolipid (SGL)-1 (glucuronosyl ceramide), which is characteristic sphingomonads. In addition, three (Sphingomonas yanoikuyae, terrae macrogoltabidus) showed second SGL (SGL-1')...
Cord factor (trehalose 6,6'-dimycolate, TDM) is an unique glycolipid with a trehalose and two molecules of mycolic acids in the mycobacterial cell envelope. Since TDM consists very long branched-chain 3-hydroxy fatty acids, molecular mass ranges widely complex manner. To characterize structure precisely simply, attempt was made to determine acid subclasses species composition intact by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectrometry for first time. The...
On the basis of phenotypic and genotypic characteristics 16S rRNA gene sequence analysis, novel species belonging to genera Sphingomonas Brevundimonas were identified from samples taken Russian space laboratory Mir. Strain A1-18 T was isolated air. rDNA analysis showed that strain formed a coherent cluster with sanguinis , parapaucimobilis paucimobilis roseiflava similarity 97·5–98·6 %. Similar other species, G+C content 66·1 mol%, but DNA–DNA hybridization rates at optimal temperatures...
A polyphasic taxonomic study was performed on the phytopathogenic bacterial strains DAPP-PG 224(T) and 228, which cause brown spot yellow Spanish melon (Cucumis melo var. inodorus) fruits. Based presence of glucuronosyl ceramide (SGL-1) in cellular lipids, results fatty acid analysis 16S rDNA sequence comparison, had been identified as belonging to genus Sphingomonas phylogenetically related mali, pruni asaccharolytica. The levels similarity these three species strain were respectively 98.0,...
ABSTRACT We report the development of a serodiagnostic method for Mycobacterium avium complex (MAC) disease with an enzyme immunoassay (EIA) MAC-specific glycopeptidolipid (GPL) core as antigen. In this study, we confirmed by EIA that GPL antibody was in sera immunocompetent patients MAC disease. The quantifying evaluated clinical tool serodiagnosis pulmonary A significant increase antibodies (immunoglobulins G, A, and M) detected diseases when they were compared to who colonized MAC,...
Direct estimation of the molecular mass single species trehalose 6-monomycolate (TMM), a ubiquitous cell-wall component mycobacteria, was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectrometry. When less than 1 μg TMM analysed MALDI-TOF spectrometry, quasimolecular ions [M+Na] + each were demonstrated and numbers carbons double bonds (or cyclopropane rings) determined. Since introduction oxygen atoms such as carbonyl, methoxy ester groups yielded...
Trehalose dimycolate (TDM), also known as cord factor, is a major surface glycolipid of the cell wall mycobacteria. Because its potent biological functions in models infection, adjuvancy, and immunotherapy, it important to determine how biosynthesis regulated. Here we show that glucose, host-derived product not readily available environment, causes Mycobacterium avium down-regulate TDM expression while up-regulating production another with immunological roles T activation, glucose...
A polar multitrichous gram-negative motile rod, EY 3383, originally identified as Burkholderia thailandensis, revealed a DNA-DNA reassociation rate of 36.7%, under stringent conditions, with the type strain B. despite 16S rDNA homology value between two strains being high 97.9%. The was clearly differentiated from thailandensis by physiological, bio-chemical, and nutritional characteristics, without significant difference in cellular fatty acid lipid composition. Based on results sequence...
The genus Enhydrobacter, first reported as a member of the family Vibrionaceae, has been placed in Moraxellaceae, but incertae sedis Bergey's Manual Systematic Bacteriology 2nd edition. During our taxonomic investigation Enhydrobacter-like organisms, we observed that 16S rRNA sequences E. aerosaccus-type strain versions NCIMB 12535(T) , ATCC 27094( T) and CCUG 58314(T) were very different from accessible data (accession no. AJ550856). Phylogenetic analysis sequence revealed these organisms...
Bacillus Calmette-Guérin (BCG) Tokyo 172 is a predominant World Health Organization Reference Reagent for the BCG vaccine. Recently, substrain was reported to consist of two subpopulations with different colony morphologies, smooth and rough. Smooth colonies had characteristic 22-bp deletion in Rv3405c region difference (RD) 16 (type I), rough were complete this II). We hypothesized that morphological related lipid phenotype affects their antigenicity. determined compositions biosynthesis...
Mannans are mannose polymers attached to cell wall proteins in all Candida species, including the pathogenic fungus albicans. sensed by pattern recognition receptors expressed on innate immune cells. However, detailed structural patterns affecting sensing not fully understood because mannans have a complex structure that includes α- and β-mannosyl linkages. In this study, we focused β-1,2-mannosides of N-linked mannan C. albicans moiety is present non-pathogenic yeast Saccharomyces...
The genus Sphingobacterium , whose members are Gram-negative non-fermentative rods, possesses ceramides and related sphingophospholipids (SPLs) with isoheptadecasphinganine 2-hydroxy or non-hydroxy isopentadecanoic acid. This paper reports evidence that isolated from spiritivorum ATCC 33861 induce endonucleolytic DNA cleavage in human myeloid leukaemia HL-60 cells vitro which is the primary characteristic biochemical marker for apoptosis programmed cell death. Ceramides SPLs also induced...
A novel sphingoglycolipid was isolated from Sphingomonas yanoikuyae, and its structure identified as a galacturonosyl-beta (1-->1)-ceramide. This characteristic present in S. yanoikuyae certain other species of Sphingomonas, such mali, terrae, macrogoltabidus, but not the type paucimobilis.
Glycopeptidolipids (GPLs) are major components present on the outer layers of cell walls several nontuberculous mycobacteria. GPLs antigenic molecules and have variant oligosaccharides in mycobacteria such as Mycobacterium avium. In this study, we identified four genes (gtf1, gtf2, gtf3, gtf4) genome smegmatis. These were independently inactivated by homologous recombination M. smegmatis, structures from each gene disruptant analyzed. Thin-layer chromatography, gas chromatography-mass...
Immune adult horses have CD8(+) cytotoxic T lymphocytes (CTLs) that recognize and lyse Rhodococcus equi-infected cells in an equine lymphocyte alloantigen (ELA)-A [classical major histocompatibility complex (MHC) class I]-unrestricted fashion. As protein antigens are MHC I-restricted, the lack of restriction suggests bacterial being recognized by host not proteins. The goals this study were to test hypothesis these CTLs unique R. equi cell-wall lipids related mycobacterial lipids. Initial...
ABSTRACT The Mycobacterium avium-M. intracellulare complex (MAIC) is divided into 28 serotypes by a species-specific glycopeptidolipid (GPL). Previously, we clarified the structures of serotype 7 GPL and two methyltransferase genes ( orfA orfB ) in 12 GPL. This study elucidated chemical structure, biosynthesis gene, host innate immune response 13 oligosaccharide (OSE) structure was determined to be 4-2′-hydroxypropanoyl-amido-4,6-dideoxy-β-hexose-(1→3)-4- O -methyl-α- l -rhamnose-(1→3)-α-...