A5107481363- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
- Computational Drug Discovery Methods
- HER2/EGFR in Cancer Research
- RNA Research and Splicing
- Synthesis and biological activity
- Neuropeptides and Animal Physiology
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Protein Degradation and Inhibitors
- Receptor Mechanisms and Signaling
- Carbohydrate Chemistry and Synthesis
- Click Chemistry and Applications
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Protein Structure and Dynamics
- Pain Mechanisms and Treatments
- Healthcare and Venom Research
- Synthesis of heterocyclic compounds
- Insect and Pesticide Research
- Protein Kinase Regulation and GTPase Signaling
- Advanced Proteomics Techniques and Applications
Nagano Municipal Hospital
2024
Himeji Dokkyo University
2017-2023
Africa Rice Center
2022
Tokyo City University
2021
The University of Tokyo
1987-2017
Tokyo Denki University
2015
Toranomon Hospital
1990-2014
Kyoto Pharmaceutical University
2006-2013
University of Tsukuba
2011-2012
Systems Biology Institute
2009-2011
ErbB tyrosine kinase receptors mediate mitogenic signal cascade by binding a variety of ligands and recruiting the different cassettes adaptor proteins. In present study, we examined heregulin (HRG)-induced transduction ErbB4 receptor found that phosphatidylinositol 3'-kinase (PI3K)-Akt pathway negatively regulated extracellular signal-regulated (ERK) phosphorylating Raf-1 on Ser(259). As time-course kinetics Akt ERK activities seemed to be transient complex, constructed mathematical...
Eukaryotic translation termination is mediated by two interacting release factors, eRF1 and eRF3, which act cooperatively to ensure efficient stop codon recognition fast polypeptide release. The crystal structures of human Schizosaccharomyces pombe full-length in complex with eRF3 lacking the GTPase domain revealed details interaction between these factors marked conformational changes that occur upon binding leading resemble a tRNA molecule. Small-angle X-ray scattering analysis...
No-go decay and nonstop are mRNA surveillance pathways that detect translational stalling degrade the underlying mRNA, allowing correct translation of genetic code. In eukaryotes, protein complex Pelota (yeast Dom34) Hbs1 GTPase recognizes stalled ribosome containing defective mRNA. Recently, we found archaeal (aPelota) associates with elongation factor 1α (aEF1α) to act in pathway, which accounts for lack an ortholog archaea. Here present structure aPelota GTP-bound aEF1α determined at...
Incidental ribosome stalling during translation elongation is an aberrant phenomenon protein synthesis and subjected to quality control by surveillance systems, in which mRNA a nascent are rapidly degraded. Their detailed molecular mechanisms as well responsible factors for these processes beginning be understood. However, the initial detecting stalled that result degradation remain determined. Among identified date, two E3 ubiquitin ligases have been reported function distinct manners....
The purpose of this study was to quantitatively evaluate paradoxical diaphragmatic motion using magnetic resonance (MR) imaging. A total 27 subjects were examined, including 12 normal young adults, six control individuals, and nine patients with emphysema. With in the supine position, 30 sequential sagittal MR images entire right lung obtained during tidal deep slow breathing. Diaphragmatic movement between estimated as displacement area a respiratory cycle calculated. diaphragm,...
Diabet. Med. 29, e279‐e285 (2012) Abstract Aim To evaluate various screening criteria for pre‐diabetes to identify which combination of impaired fasting glucose and elevated HbA 1c values performs most effectively in predicting future diabetes a large cohort Japanese individuals. Methods The study included 4670 men 1571 women without (diabetes: plasma ≥ 7.0 mmol/l, 48 mmol/mol (≥ 6.5%), or self‐reported clinician‐diagnosed diabetes). Pre‐diabetes was diagnosed by (fasting 5.6–6.9 mmol/l...
The phospholipid metabolism of rat peritoneal mast cells stimulated with mastoparan, a secretagogue purified from wasp venom, was investigated. Mastoparan at 20 caused rapid secretion histamine. induced transient decrease phosphatidylinositol 4,5‐bisphosphate on 32 P labeling and generation water‐soluble degradation product, inositol trisphosphate [ 3 H]inositol labeling, suggesting the activation phospholipase C upon stimulation.
The molecular mechanisms of translation termination and mRNA surveillance in archaea remain unclear. In eukaryotes, eRF3 HBS1, which are homologous to the tRNA carrier GTPase EF1α, respectively bind eRF1 Pelota decipher stop codons or facilitate surveillance. However, genome-wide searches have failed detect any orthologs both GTPases. Here, we report crystal structure aRF1 from an archaeon, Aeropyrum pernix , present strong evidence that authentic archaeal EF1α acts as a for aPelota. binding...
Batesian mimicry protects animals from predators through resemblance with distasteful models in shape, color pattern, or behavior. To elucidate the wing coloration mechanisms involved mimicry, we investigated chemical composition and gene expression of pale yellow red pigments a swallowtail butterfly, Papilio polytes, whose females mimic unpalatable butterfly Pachliopta aristolochiae. Using LC/MS, showed that regions non-mimetic consist kynurenine N-β-alanyldopamine (NBAD). Moreover, qRT-PCR...
The extracellular domain of human EGF receptor (sEGFR) produced by CHO cells has been used in various biophysical studies to elucidate the molecular mechanism EGF-induced activation. We have found that sEGFR contains one oligosac-charide chain attached an atypical N-glycosylation consensus sequence, Asn32 -X33 -Cys34. oligosaccharide structure at is a mixture monosialo and asialo forms core fucosylated biantennary complex-type oligosaccharide. Deletion this glycosylation site replacement...
In the postgenomic era, many researchers and organizations have been engaged in structural functional analyses of proteins. As a part these efforts, searching for small organic compounds that bind specifically to target proteins is quite important. this study, we developed rational strategy ligand discovery based on three-dimensional structures proteins, which were elucidated by X-ray crystallography nuclear magnetic resonance spectroscopy. The has three features: (i) rapid selection...
When a stop codon appears at the ribosomal A site, class I and II release factors (RFs) terminate translation. In eukaryotes archaea, RFs form heterodimeric complex, complete overall translation termination process in GTP-dependent manner. However, structural mechanism of by RF complex remains unresolved. archaeal elongation factor 1 alpha (aEF1α), carrier GTPase for tRNA, acts as forming with RF1 (aRF1). We report crystal structure aRF1·aEF1α first active complex. This remarkably resembles...