A5101762669- Chemical Synthesis and Reactions
- Chemical Synthesis and Analysis
- Oxidative Organic Chemistry Reactions
- Analytical Chemistry and Chromatography
- Synthetic Organic Chemistry Methods
- Asymmetric Synthesis and Catalysis
- Synthesis and Catalytic Reactions
- Neurobiology and Insect Physiology Research
- Metabolomics and Mass Spectrometry Studies
- Angiogenesis and VEGF in Cancer
- Pharmacogenetics and Drug Metabolism
- Mass Spectrometry Techniques and Applications
- Animal Behavior and Reproduction
- Neuroscience and Neuropharmacology Research
- Inorganic and Organometallic Chemistry
- Photosynthetic Processes and Mechanisms
- Lepidoptera: Biology and Taxonomy
- Advanced Synthetic Organic Chemistry
- Asymmetric Hydrogenation and Catalysis
- Cancer, Hypoxia, and Metabolism
- Receptor Mechanisms and Signaling
- Synthesis of heterocyclic compounds
- Drug Transport and Resistance Mechanisms
- Synthesis and Biological Evaluation
- Fluorine in Organic Chemistry
Taisho Pharmaceutical (Japan)
2003-2023
Kanagawa Institute of Technology
2005-2023
Sakai Chemical Industry (Japan)
2020
The University of Tokyo
2011-2019
Aix-Marseille Université
2018
Centre National de la Recherche Scientifique
2018
Institut de Biologie du Développement Marseille
2018
Keihin (Japan)
2013
Osaka University
2010
Tufts University
2001-2003
Background —We investigated the therapeutic potential of ex vivo expanded endothelial progenitor cells (EPCs) for myocardial neovascularization. Methods and Results —Peripheral blood mononuclear obtained from healthy human adults were cultured in EPC medium harvested 7 days later. Myocardial ischemia was induced by ligating left anterior descending coronary artery male Hsd:RH-rnu (athymic nude) rats. A total 10 6 EPCs labeled with 1,1′-dioctadecyl-1 to 3,3,3′,3′-tetramethylindocarbocyanine...
Stromal cell-derived factor-1 (SDF-1) is a chemokine considered to play an important role in the trafficking of hematopoietic stem cells. Given close relationship between cells and endothelial progenitor (EPCs), we investigated effect SDF-1 on EPC-mediated vasculogenesis.Flow cytometric analysis demonstrated expression CXCR4, receptor SDF-1, by 66+/-3% EPCs after 7 days culture. In vitro modified Boyden chamber assay showed dose-dependent EPC migration toward (control versus 10 ng/mL 100...
Previous studies have established that bone marrow-derived endothelial progenitor cells (EPCs) are present in the systemic circulation. In current study, we investigated hypothesis gene transfer can be used to achieve phenotypic modulation of EPCs.In vitro, ex vivo murine vascular growth factor (VEGF) 164 augmented EPC proliferative activity and enhanced adhesion incorporation EPCs into quiescent as well activated cell monolayers. To determine if such may facilitate therapeutic...
In Batesian mimicry, animals avoid predation by resembling distasteful models. the swallowtail butterfly Papilio polytes, only mimetic-form females resemble unpalatable Pachliopta aristolochiae. A recent report showed that a single gene, doublesex (dsx), controls this mimicry; however, detailed molecular mechanisms remain unclear. Here we determined two whole-genome sequences of P. polytes and related species, xuthus, identifying ∼130-kb autosomal inversion, including dsx, between mimetic...
Many animal species comprise discrete phenotypic forms. A common example in natural populations of insects is the occurrence different color patterns, which has motivated a rich body ecological and genetic research [1-6]. The dark, i.e., melanic, forms displaying patterns found across multiple taxa, but underlying genomic basis remains poorly characterized. In numerous ladybird (Coccinellidae), spatial arrangement black red patches on adult elytra varies wildly within species, forming...
Short interference RNA (siRNA) is widely used in mammalian cells. In insects, however, reports concerning the suitablility of siRNA vivo very limited compared with that long dsRNA, which thought to be more effective. There insufficient information on essential rules design as few siRNAs have been tested this context. To establish an effective method gene silencing using we determined effects seven target genes. We designed according a new guideline and injected them into eggs Bombyx mori. At...
Batesian mimicry protects animals from predators through resemblance with distasteful models in shape, color pattern, or behavior. To elucidate the wing coloration mechanisms involved mimicry, we investigated chemical composition and gene expression of pale yellow red pigments a swallowtail butterfly, Papilio polytes, whose females mimic unpalatable butterfly Pachliopta aristolochiae. Using LC/MS, showed that regions non-mimetic consist kynurenine N-β-alanyldopamine (NBAD). Moreover, qRT-PCR...
Significance A stripe pattern is widely observed among animals and often used for warning or camouflage in caterpillars. However, its genetic background largely unknown. This study showed that the Toll ligand Spätzle3 (Spz-3) responsible silkworm Zebra locus, which causes black stripes on anterior margin of each segment. Exhaustive knockdown experiments spz- Toll- related genes clarified spz-3 Toll-8 are involved melanin pigmentation another mutant. The Spz-3/Toll-8 signaling pathway...
Color patterns often function as camouflage to protect insects from predators. In most swallowtail butterflies, younger larvae mimic bird droppings but change their pattern host plants during final molt. This is determined the early fourth instar by juvenile hormone (JH-sensitive period), it remains unclear how prepatterning process controlled. Using Papilio xuthus larvae, we performed transcriptome comparisons identify three pattern-associated homeobox genes [clawless, abdominal-A, and...
Abstract Two diastereomers of d-limonene-derived five-membered cyclic carbonates were prepared from the corresponding isomers d-limonene oxide with CO2. Their syntheses catalyzed by commercially available tetrabutylammonium chloride high stereoselectivity. The reaction behavior dependent on conditions such as CO2 pressure was clarified.
In order to produce versatile and potentially functional terpene-based compounds, a (R)-limonene-derived diol its corresponding five-membered cyclic carbonate were prepared. The (cyclic carbonate) comprises four diastereomers based on the stereochemical configuration of (and moiety. By choosing appropriate starting compounds (trans- cis-limonene oxide) conditions, desired synthesised in moderate high yields with, most cases, stereoselectivity. Comparison NMR data obtained diols carbonates...
The need for not only bulk sensitive but also extremely high resolution photoelectron spectroscopy studying detailed electronic structures of strongly correlated electron systems is growing rapidly. Moreover, easy access to such a capability in one’s own laboratory desirable. Demonstrated here the performance microwave excited rare gas (Xe, Kr, and Ar) lamp combined with ionic crystal filters (sapphire, CaF2, LiF), which can supply three strong lines near photon energy hnyu hν=8.4, 10.0,...
Abstract The oxidation of secondary amines with copper(II) reagent, prepared from bromide and lithium t-butoxide, under mild reaction conditions gave the corresponding imines in high yields. Primary were also transformed to nitriles.
Detection of protein–protein interactions is a fundamental step to understanding gene function. Here we report sensitive and rapid method for assaying at the single‐molecule level. Protein molecules were synthesized in cell‐free translation system presence Cy5‐puro, fluorescent puromycin, using mRNA without stop codon. The interaction proteins thus prepared was visualized imaging technique. As demonstration this method, motor protein, kinesin, labeled with Cy5‐puro an efficiency about 90%,...
New plant production systems with a low environmental impact (or high resource utilization efficiency) are necessary for urban agriculture development. This study explores the benefits of closed (CPPSs) artificial lighting using commercial CPPS at Osaka Prefecture University. Lettuce plants were produced continuously as model analyzing consumption rates, such electricity, labor, water, and CO2, over two years. Monthly rates CO2 increased increase in monthly rate lettuce. The efficiencies...
Allylgermanes were obtained by the reaction of allyl acetates with bis(triethylgermyl)cuprate(I) reagent in high yields. It was found that present proceeded regioselectivity, which a triethylgermyl group exclusively introduced to less substituted side an allylic system regardless original location acetoxyl group. In case possessing two secondary centers at both ends system, formal SN2 product preferentially produced (SN2 : SN2′ = ca. 9 1). The desulfurizative germylation phenyl sulfides...
Three distinct classes of nuclear receptors, EcR, E75, and HR3, are key regulators in the ecdysone-inducible gene activation cascade insects. The transcription these genes is induced by ecdysone (20E) differently, although detailed mechanisms underlying their responses to 20E largely unknown. We identified response elements (EcREs) present promoters coding BmEcR-B1, BmE75-A, BHR3-B isoforms from Bombyx mori employing luciferase reporter assays an ecdysteroid-responsive cultured cell line,...
A strategy for highly sensitive metabolite screening by liquid chromatography-electrospray ionization (ESI) mass spectrometry with the negative-ion mode that involves use of a reversed-phase column in gradient-elution and postcolumn addition 2-(2-methoxyethoxy)ethanol (2-MEE), novel signal-enhancing modifier, has been described. When mobile phase 50 mM ammonium acetate/acetic acid buffer (pH 4.4) at flow rate 100 microL/min was employed, poor ESI response ibuprofen as model drug, probably...