A5067888688- Mycobacterium research and diagnosis
- Actinomycetales infections and treatment
- Infectious Diseases and Mycology
- Tuberculosis Research and Epidemiology
- Fungal Infections and Studies
- Infectious Diseases and Tuberculosis
- Leprosy Research and Treatment
- Nail Diseases and Treatments
- Mycorrhizal Fungi and Plant Interactions
- Antifungal resistance and susceptibility
- Plant Pathogens and Fungal Diseases
- Antibiotic Resistance in Bacteria
- Yersinia bacterium, plague, ectoparasites research
- Genomics and Phylogenetic Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Cutaneous lymphoproliferative disorders research
- Cancer therapeutics and mechanisms
- Diagnosis and treatment of tuberculosis
- Infectious Disease Case Reports and Treatments
- Liver Disease Diagnosis and Treatment
- Infective Endocarditis Diagnosis and Management
- Research on Leishmaniasis Studies
- Marine Sponges and Natural Products
- Fibroblast Growth Factor Research
- Quinazolinone synthesis and applications
Hospital Universitario Dr José Eleuterio Gonzalez
2013-2025
Universidad Autónoma de Nuevo León
2014-2024
Colorado State University
2022
Swiss Tropical and Public Health Institute
2022
University of Basel
2022
Secretaría de Salud de Jalisco
2022
University Dermatology
2010
Institute of Dermatology
2002-2004
Health Canada
1999
Leprosy, caused by infection with Mycobacterium leprae or the recently discovered lepromatosis, was once endemic in humans British Isles. Red squirrels Great Britain (Sciurus vulgaris) have increasingly been observed leprosy-like lesions on head and limbs. Using genomics, histopathology, serology, we found M. lepromatosis from England, Ireland, Scotland, Brownsea Island, England. Infection detected overtly diseased seemingly healthy animals. Phylogenetic comparisons of Irish two Mexican...
Significance Leprosy was thought to be exclusively caused by infection of humans Mycobacterium leprae . In 2008, Han et al. proposed that lepromatosis , a separate unculturable species, might responsible for rare yet severe form the disease called diffuse lepromatous leprosy. Here, using comparative genomics, we show two species are very closely related and derived from common ancestor underwent genome downsizing gene decay. Since their separation 13.9 Mya, have continued lose genes, but...
Leprosy is a chronic human disease caused by the yet-uncultured pathogen Mycobacterium leprae. Although readily curable with multidrug therapy (MDT), over 200,000 new cases are still reported annually. Here, we obtain M. leprae genome sequences from DNA extracted directly patients' skin biopsies using customized protocol. Comparative and phylogenetic analysis of 154 genomes 25 countries provides insight into evolution antimicrobial resistance, uncovering lineages phylogeographic trends, most...
Background Mycetoma is a neglected tropical disease characterized by nodules, scars, abscesses, and fistulae that drain serous or purulent material containing the etiological agent. may be caused true fungi (eumycetoma) filamentous aerobic bacteria (actinomycetoma). more frequent in so-called mycetoma belt (latitude 15° south 30° north around Tropic of Cancer), especially Sudan, Nigeria, Somalia, India, Mexico, Venezuela. The introduction new antibiotics with fewer side effects, broader...
Summary In the present report we reviewed a total of 2397 cases dermatophytosis from superficial cutaneous lesions between years 1978 and 1990. The included were Department Dermatology at University Hospital located in city Monterrey, México. A 726 tinea pedis, 613 unguium, 441 capitis, 395 corporis 222 cruris observed. most commonly isolated dermatophyte species was Trichophyton rubrum (45%), followed by mentagrophytes (23.7%), tonsurans (21%), Microsporum canis (7.1%) Epidermophyton...
The in vitro activities of DA-7157, a novel oxazolidinone, against clinical isolates Nocardia brasiliensis and Mycobacterium tuberculosis were determined. Equal MIC(50)s MIC(90)s (0.25 0.5 microg/ml, respectively) found for susceptible multidrug-resistant M. tuberculosis. N. showed an MIC(90) 1 microg/ml MIC(50) microg/ml. DA-7157 prodrug, DA-7218, exhibited similar MICs but fivefold-higher brasiliensis.
An 86-year-old female patient from northeast Mexico presented with diffuse lepromatous leprosy (DLL). Sequence analysis of four genes (rrs, rpoB, sigA, and hsp65) the skin biopsy specimen identified "Mycobacterium lepromatosis." This is first independent confirmation a case DLL due to M. lepromatosis.
Nocardia brasiliensis is an important etiologic agent of mycetoma. These bacteria live as a saprobe in soil or organic material and enter the tissue via minor trauma. Mycetoma characterized by tumefaction production fistula abscesses, with no spontaneous cure. By using mass sequencing, we determined complete genomic nucleotide sequence bacteria. According to our data, genome circular chromosome 9,436,348-bp long 68% G+C content that encodes 8,414 proteins. We observed orthologs for virulence...
Actinomadura isolates obtained from seven human mycetoma cases in Mexico were characterized using nucleotide sequence analysis of a portion the small subunit ribosomal RNA gene. Most identified as madurae. However, one isolate, LIID-AQ337, showed inconclusive results. To determine its identity, genomic DNA LIID-AQ337 was subjected to whole-genome sequencing both short- and long-read sequencing, resulting genome 8,163,638-bp spread 22 contigs. Comparative against currently available genomes...
Abstract Background Antimicrobials are essential in acne therapy. In the last decades, Propionibacterium acnes has become resistant to different antibiotics. Objective To determine antimicrobial susceptibility patterns of P. frequently used drugs. Materials and methods Cutaneous lesion samples were obtained from 50 patients with vulgaris, which cultured anaerobic media demonstrate presence . After that, tests tetracycline, minocycline, doxycycline, erythromycin, azithromycin, clindamycin,...
Eurasian red squirrels (Sciurus vulgaris) in the British Isles are most recently discovered animal reservoir for leprosy bacteria Mycobacterium leprae and lepromatosis. Initial data suggest that prevalence of infection is variable often low different squirrel populations. Nothing known about presence bacilli other wild species despite two others (Siberian chipmunk [Tamias sibiricus], Thirteen-lined ground [Ictidomys tridecemlineatus]) having been reported to be susceptible experimental with...
In Mexico mycetomas are mostly produced by Nocardia brasiliensis, which can be isolated from about 86% of cases. the present work, we determined sensitivities 30 N. brasiliensis strains patients with mycetoma to several groups antimicrobials. As a first screening step carried out disk diffusion assays 44 antimicrobials, including aminoglycosides, cephalosporins, penicillins, quinolones, macrolides, and some others. these observed that antimicrobials have an effect on more than 66% strains:...
ABSTRACT Although tuberculosis is still a public health problem in Mexico, there little information about the genetic characteristics of isolates. In present study, we analyzed by spoligotyping 180 Mycobacterium clinical isolates from urban area Monterrey, including drug-susceptible and drug-resistant The spoligotype patterns were compared with those international SITVIT2 database. Four presented not found database (orphan types); rest distributed among 44 spoligo types (SITs). SIT53 (clade...
Nocardia brasiliensis is a Gram-positive facultative intracellular bacterium frequently isolated from human actinomycetoma. However, the pathogenesis of this infection remains unknown. Here, we used model bacterial delipidation with benzine to investigate role N. cell wall-associated lipids in experimental Delipidation resulted complete abolition actinomycetoma without affecting viability. Chemical analyses revealed that trehalose dimycolate and an unidentified hydrophobic compound were...
Due to the emergency of multidrug-resistant strains Mycobacterium tuberculosis, is necessary evaluation new compounds.Tedizolid, a novel oxazolidinone, and ACH-702, isothiazoloquinolone, were tested against M. tuberculosis infected THP-1 macrophages. These two compounds significantly decreased number intracellular mycobacteria at 0.25X, 1X, 4X 16X MIC value. The drugs either in nanoparticules or free solution.Tedizolid ACH-702 have good killing activity comparable that rifampin moxifloxacin.
In Mexico, actinomycetoma is mainly caused by Nocardia brasiliensis, which a soil inhabitant actinobacterium. Here, we report for the first time draft genome of strain isolated from human case that has largely been found in vitro and experimental models actinomycetoma, N. brasiliensis HUJEG-1.
Mycobacterium tuberculosis is a pathogen causing (TB) spectrum of disease including acute and asymptomatic latent stages. Identifying treating latently-infected patients constitutes one the most important impediments to TB control efforts. Those individuals can remain undiagnosed for decades serving as potential reservoirs reactivation. Tests accurate diagnosis infection currently are unavailable. HspX protein (α-crystallin), encoded by Rv2031c gene, produced in vitro M. during stationary...