A5026874509- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Amoebic Infections and Treatments
- Parasitic Infections and Diagnostics
- Antibiotic Resistance in Bacteria
- Infectious Diseases and Tuberculosis
- Infectious Diseases and Mycology
- Pancreatitis Pathology and Treatment
- Diagnosis and treatment of tuberculosis
- Essential Oils and Antimicrobial Activity
- Research in Cotton Cultivation
- Helicobacter pylori-related gastroenterology studies
- Moringa oleifera research and applications
- Toxin Mechanisms and Immunotoxins
- 3D Printing in Biomedical Research
- Sphingolipid Metabolism and Signaling
- Cancer therapeutics and mechanisms
- Actinomycetales infections and treatment
- Neurological Disease Mechanisms and Treatments
- Herpesvirus Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Vanadium and Halogenation Chemistry
- Streptococcal Infections and Treatments
- Lipid Membrane Structure and Behavior
- Leptospirosis research and findings
Universidad Autónoma de Nuevo León
2000-2025
Mexican Social Security Institute
2012-2022
Centers for Disease Control and Prevention
2002
National Center for Infectious Diseases
2002
Jamia Millia Islamia
2002
Tuberculosis, caused by Mycobacterium tuberculosis, is a major global health problem. In Mexico, the State of Nuevo Leon among top ten in tuberculosis morbidity. Information about transmission patterns and case clustering for limited. The spoligotypes 151 isolates from newly diagnosed pulmonary patients were obtained its phenotypic drug susceptibility pattern streptomycin, isoniazid, rifampin, ethambutol, pyrazinamide was determined using Mycobacteria Growth Indicator Tube fluorometric...
Mycobacterium tuberculosis is a serious worldwide health threat, killing almost 2 million people per year. Alternative antimycobacterial drugs are urgently needed; studies have shown that medicinal plants traditionally used to treat respiratory diseases potential source of compounds tuberculosis. This paper studied the activity 28 extracts from four different plant species been in traditional Mexican medicine Bark and leaf crude Juglans regia L., mollis Engelm., Carya illinoensis (Wangenh)...
Journal Article PEHPS medium: An alternative for axenic cultivation of Entamoeba histolytica and E. invadens Get access Salvador Said-Fernández, Said-Fernández ∗ 1División de Biología Celular, Unidad Investigación Biomédica del Noreste, Instituto Mexicano Seguro Social, Apartado Postal 020-E, 64720, Monterrey, Nuevo León, México ∗Author correspondence. Search other works by this author on: Oxford Academic PubMed Google Scholar Javier Vargas-Villarreal, Vargas-Villarreal Jorge Castro-Garza,...
Due to the emergency of multidrug-resistant strains Mycobacterium tuberculosis, is necessary evaluation new compounds.Tedizolid, a novel oxazolidinone, and ACH-702, isothiazoloquinolone, were tested against M. tuberculosis infected THP-1 macrophages. These two compounds significantly decreased number intracellular mycobacteria at 0.25X, 1X, 4X 16X MIC value. The drugs either in nanoparticules or free solution.Tedizolid ACH-702 have good killing activity comparable that rifampin moxifloxacin.
Mycobacterium tuberculosis is a pathogen causing (TB) spectrum of disease including acute and asymptomatic latent stages. Identifying treating latently-infected patients constitutes one the most important impediments to TB control efforts. Those individuals can remain undiagnosed for decades serving as potential reservoirs reactivation. Tests accurate diagnosis infection currently are unavailable. HspX protein (α-crystallin), encoded by Rv2031c gene, produced in vitro M. during stationary...
We developed an in vitro tissue-culture model to analyze the process involved mycobacterial spread through lung epithelial cell monolayers. A549 cells were infected with low numbers of viable Mycobacterium tuberculosis bacilli expressing gfp gene. Subsequent addition a soft agarose overlay prevented dispersal from initial points attachment. By fluorescence microscopy bacteria observed infect and grow within primary target cells; this was followed by lysis subsequent infection adjacent cells....
Several techniques have been used to quantify the cytotoxicity produced by Mycobacterium tuberculosis bacilli on cell monolayers; however, they are semi-quantitative or time consuming. Herein, a method based crystal violet (CV) uptake THP-1 monolayers is described. This colorimetric quantifies cytotoxic effect as function of number remaining cells after infection with M. . Since this micro-organism not stained dye, it does produce background that affects absorbance readings. As determined CV...
ABSTRACT Enzymes with phospholipase C activity in Mycobacterium tuberculosis have been recently described. The three genes encoding these proteins, plcA , plcB and plcC are located at position 2351 of the genomic map M. H37Rv arranged tandem. We previously described presence variations restriction fragment length polymorphism patterns clinical isolates. In present work we investigated origin this by sequence analysis phospholipase-encoding regions 11 polymorphic To do so, a long-PCR assay...
Hedeoma drummondii (poleo) has been used by Mexicans to prepare a tea for its pleasant taste. The methanolic extract, polar fractions, and major antioxidant compounds of the Mexican herbal obtained from aerial parts H. ("poleo") showed antiproliferative effects against cancer cell lines MCF-7 (human breast) HeLa cervix). Phenolic acids isolated poleo have significant with caffeic acid most potent. This evidence suggests that may chemopreventive properties.
Abstract Background Multidrug-resistant infections due to Mycobacterium abscessus often require complex and prolonged regimens for treatment. Here, we report the evaluation of a new ex vivo antimicrobial susceptibility testing model using organotypic cultures murine precision-cut lung slices, an experimental in which metabolic activity, all usual cell types organ are found while tissue architecture interactions between different cells maintained. Methods Precision cut slices (PCLS) were...
Tuberculosis remains a serious threat to human health as an infectious disease in Mexico. Data about the genotypes of circulating Mycobacterium tuberculosis isolates (MTB) State Nuevo Leon, Mexico are scarce. We aimed determine MTB belonging Beijing lineage recovered from patients A total 406 this state were genotyped using spoligotyping method and 18-locus MIRU-VNTR. Lineage classification transmission analysis performed. Based on analysis, we found 24 strains genotype that characterized...
Mycobacterium tuberculosis strains CDC1551 and Erdman were used to assess cytotoxicity in infected A549 human alveolar epithelial cell monolayers. Strain was found induce qualitatively greater disruption of monolayers than strain Erdman, although total intracellular cell-associated bacterial growth rates over the course infections not significantly different. Cell-free culture supernatants from monocytic cells with either 2 M. produced a cytotoxic effect on cells, correlating amount tumor...
Mycetoma is a neglected, chronic, and deforming infectious disease caused by fungi actinomycetes. In Mexico, N. brasiliensis the predominant etiologic agent. Therapeutic alternatives are necessary because current drug regimens have several disadvantages. Benzothiazinones (BTZ) new class of candidate drugs that inhibit decaprenyl-phosphoribose-epimerase (DprE1), an essential enzyme involved in cell wall biosynthesis Corynebacterineae.In this study, vitro activity next generation BTZ, PBTZ169,...
BackgroundNocardia are organisms that can escape the effects of both immune response and antimicrobial agents, due to their potential capacity grow intracellularly. In previous studies, we found experimental oxazolidinones, DA-7157 DA-7218, active in vitro vivo.
Continuous subculture has been observed to produce changes in the virulence of micro-organisms, e.g. rabies virus, poliovirus and Mycobacterium bovis BCG. The latter used as a vaccine for tuberculosis last 100 years; however, some instances its efficacy be very low. In order determine whether similar can produced tuberculosis, we selected four isolates, M. H37Rv, Beijing strain (DR-689), two more isolates with deletion phospholipase C locus (plcA-plcB-plcC ), subjected them serial culturing...
Introduction Group B Streptococcus (GBS) causes infections in women during pregnancy and puerperium invasive newborns. The genes lmb , cylE scpB hvgA are involved with increased virulence of GBS, hypervirulent clones have been identified different regions. In addition, increasing resistance GBS to macrolides lincosamides has reported, so knowing the patterns antibiotic may be necessary prevent treat infections. This study aimed identify associated colonization pregnant from northeastern...
The in vitro activities of DA-7867, a novel oxazolidinone, and garenoxacin (BMS-284756) were compared to those linezolid 67 susceptible drug-resistant clinical isolates Mycobacterium tuberculosis. DA-7867 was the most active drug with an MIC(90) 0.125 microg/ml, MIC(90)s 4 microg/ml 2 linezolid.