An emerging concern is the influences of early life exposure to environmental toxicants on offspring characteristics in later life. Since recent evidence suggests a transgenerational transference aberrant phenotypes from exposed-parents non-exposed related adult-onset diseases including reproductive phenotype. The potential arsenic well know genotoxic and epigenetic modifier agent has not been assessed mammals until now. In this experimental study, we evaluated effects rat model with chronic...

Physalis angulata is a plant of great value in traditional medicine known for its content bioactive compounds, such as physalins and withanolides, which possess diverse biological activities. In this study, the chemical profile, antioxidant activity, enzyme inhibition capacity aqueous ethanolic extracts obtained from root, stem, leaves, calyx, fruits P. collected Peru were evaluated. A total forty-two compounds detected using UHPLC-ESI-QTOF-MS analysis. vitro analyses revealed that leaf...

Breast cancer is the leading cause of death in women worldwide. The search for novel compounds with antitumor activity, less adverse effects and higher efficacy, development methods to evaluate their toxicity an area ​​intense research. In this study we implemented preparation culture breast tumor explants, which were obtained from precision-cut slices. order validate model are proposing screen antineoplastic effect natural compounds, selected caffeic acid, ursolic rosmarinic acid. Using...

Mycobacterium tuberculosis is a serious worldwide health threat, killing almost 2 million people per year. Alternative antimycobacterial drugs are urgently needed; studies have shown that medicinal plants traditionally used to treat respiratory diseases potential source of compounds tuberculosis. This paper studied the activity 28 extracts from four different plant species been in traditional Mexican medicine Bark and leaf crude Juglans regia L., mollis Engelm., Carya illinoensis (Wangenh)...

Background:Currently, one of the most used strategies for treatment newly diagnosed patients with breast cancer is neoadjuvant chemotherapy based on application taxanes and anthracyclines. However, despite high number who develop a complete pathological clinical response, resistance relapse following this therapy continue to be challenge. As component innate immune system, cytotoxic function Natural Killer (NK) cells plays an important role in elimination tumor cells. NK systemic remains...

Hexane, acetone and methanol extracts obtained from Psidium guajava leaves were studied for their antifungal properties against Trichophyton rubrum, tonsurans, Sporotrix schenckii, Microsporum canis, Cryptococcus neoformans, Candida parapsilosis,and albicans by using the agar disk diffusion technique. Compared to control, hexane extract showed best activity, being active all tested dermatophytes. Methanol also relevant activity. The phytochemical analysis of revealed presence flavonoids,...

Several techniques have been used to quantify the cytotoxicity produced by Mycobacterium tuberculosis bacilli on cell monolayers; however, they are semi-quantitative or time consuming. Herein, a method based crystal violet (CV) uptake THP-1 monolayers is described. This colorimetric quantifies cytotoxic effect as function of number remaining cells after infection with M. . Since this micro-organism not stained dye, it does produce background that affects absorbance readings. As determined CV...

The micronucleus (MN) assay evaluates the effects of low doses genotoxic carcinogens and can detect structural lesions that survive mitotic cycles.The objective this study was to determine both genotoxicity nickel (Ni) in buccal epithelial cells urinary excretion Ni children with metal crowns.This a prospective longitudinal based on 37 patients selected at Facultad de Odontología la Universidad Autónoma Coahuila.MN assays were performed using from patients, levels determined urine samples...

Hedeoma drummondii (poleo) has been used by Mexicans to prepare a tea for its pleasant taste. The methanolic extract, polar fractions, and major antioxidant compounds of the Mexican herbal obtained from aerial parts H. ("poleo") showed antiproliferative effects against cancer cell lines MCF-7 (human breast) HeLa cervix). Phenolic acids isolated poleo have significant with caffeic acid most potent. This evidence suggests that may chemopreventive properties.

Abstract Differentiated cells telomere length is an indicator of senescence or lifespan; however, in peripheral blood leukocytes the relative shortening has been considered as a biological marker aging, and lengthening associated risk to cancer. Individual’s age, type tissue, lifestyle, environmental factors make variable. The presence carcinogens such arsenic (As) influence causal agents these alterations, main modes action for As described are oxidative stress, reduction DNA repair...

Macroalgae represent an important source of bioactive compounds with a wide range biotechnological applications. Overall, the discovery effective cytotoxic pharmaceutical potential is significant challenge, mostly because they are scarce in nature or their total synthesis not efficient, while bioprospecting models currently used do predict clinical responses. Given this context, we three-dimensional (3D) cultures human breast cancer explants to evaluate antitumoral effect laurinterol, major...

Marine environments represent a great opportunity for the discovery of compounds with wide spectrum bioactive properties. Due to their large variety and functions derived from natural selection, marine products may allow identification novel drugs based not only on newly discovered metabolites but also already known yet thoroughly investigated. Since drug resistance has caused an increase in infections by Mycobacterium tuberculosis nontuberculous mycobacteria, re-evaluation been suggested as...

Abstract Background Multidrug-resistant infections due to Mycobacterium abscessus often require complex and prolonged regimens for treatment. Here, we report the evaluation of a new ex vivo antimicrobial susceptibility testing model using organotypic cultures murine precision-cut lung slices, an experimental in which metabolic activity, all usual cell types organ are found while tissue architecture interactions between different cells maintained. Methods Precision cut slices (PCLS) were...

Many hepatoprotective herbal preparations have been recommended in alternative systems of medicine for the treatment hepatic disorders. No systematic study has done on protective efficacy Leucophyllum frutescens to treat diseases. Protective action L. methanol extract (obtained by maceration) was evaluated an animal model hepatotoxicity induced carbon tetrachloride (CCl(4)). Wistar albino rats were divided into five groups. Group I normal control group; Groups II-V received CCl(4). After...

Abstract Objectives This study was undertaken to investigate the amoebicidal potential of curcumin on Entamoeba histolytica, as well its synergistic effect with metronidazole. Methods histolytica trophozoites were exposed 100, 200 and 300 μm curcumin, for 6, 12 24 h. Consequently, viability cells determined by trypan blue exclusion test. All specimens further analysed scanning electron microscopy. For drug combination experiment, Chou-Talalay method used. Key findings Curcumin affected...

Linnaeus (Boraginacea) and

Los cambios cardiovasculares del embarazo conllevan mayor riesgo en cardiópatas. El objetivo fue analizar los efectos adversos cardiovasculares, obstétricos y perinatales asociados a cardiopatía congénita adquirida durante el puerperio. Estudio transversal retrospectivo, que incluyó registro de 2017-2023 pacientes embarazadas o puérperas hospitalizadas con diagnóstico adquirida. Se compararon eventos (insuficiencia cardiaca, evento vascular cerebral, edema agudo pulmonar, muerte materna,...